Oxyresveratrolün, Deneysel Yaşa Bağli Makula Dejenerasyonunda Oksidatif Strese Karşi Koruyucu Etkisi

Year 2022, , 320 - 327, 30.12.2022

Cansu Kara Öztabağ , Akif Hakan Kurt , Lokman Ayaz , Mehmet Ali Sungur

https://doi.org/10.47493/abantmedj.1213603

Abstract

Amaç: Yaşa bağlı makula dejenerasyonu (YBMD), retina pigment epitel kompleksinin nörodejenerasyonunun neden olduğu görme kaybı ile karakterize kronik bir hastalıktır. Fazla oluşan reaktif oksijen türleri (ROS), makula dejenerasyonu başta olmak üzere retina hastalıklarının gelişmesinde önemli rol oynar. Başlıca ROS’lar ise; peroksinitritler, süperoksit radikaller ve hidrojen peroksitlerdir. Çalışmamızda hücre kültürü ortamında hidrojen peroksit (H2O2) ile oluşturulan oksidatif hasar öncesi oksiresveratrolün koruyucu etkisini araştırmayı amaçladık.
Gereç ve Yöntemler: İnsan retina pigment epitel (ARPE-19) hücrelerinde H2O2 ile oksidatif stres oluşturuldu. Oksidatif hasar öncesi oksiresveratrol 7 farklı konsantrasyonda uygulandı. Koruyucu etkiler, XTT hücre proliferasyonu testi ile hücre canlılığındaki değişiklik izlenerek araştırıldı. Oksiresveratrolün koruyucu etkisini moleküler düzeyde araştırmak için kaspaz-3 ve hücre ölüm tespit kiti kullanıldı.
Bulgular: Çalışmamızda ARPE-19 hücre hattında H2O2 ile oluşturulan oksidatif hasar öncesi oksiresveratrol uygulaması hücre canlılığını arttırarak hücrede oksidatif hasara karşı koruyucu etkinlik göstermiştir. Çalışmamız sonucunda elde ettiğimiz bulgularda; oksiresveratrol ARPE-19 hücrelerinde H2O2 ile oluşturulan oksidatif hasar oluşum öncesi uygulandığında 100 µM konsantrasyonda hücre hasarını yaklaşık %15 oranında azaltmıştır, buna ek olarak, hücre ölüm tespiti ve kaspaz-3 sonuçlarına göre, oksiresveratrolün oksidatif hasara karşı apoptotik hücre ölümünü azaltarak koruyucu etkinlik göstermektedir.
Sonuç: Bu in vitro çalışma oksiresveratrolün koruyucu etkisinin geliştirilmesi için ön çalışma niteliğindedir. Oksiresveratrol, deney hayvanları ve klinik çalışmalar sonrasında, başta YBMD olmak üzere retina hastalıklarının önlenmesinde etkin bir terapötik ajan olarak geliştirilebilir.

Keywords

ARPE-19, Oksidatif Stres, Oksiresveratrol, Yaşa Bağlı Makula Dejenerasyonu

Supporting Institution

Bolu Abant İzzet Baysal Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

Proje numarası: 2020.08.36.1477

Protectıve Effect Of Oxyresveratrol Agaınst Oxıdatıve Stress In Experımental Age-Related Macula Degeneratıon

Abstract

Objective: Age-related-macular-degeneration (AMD) is a chronic disease characterized by vision loss caused by neurodegeneration of the retinal pigment epithelial complex. Excess reactive oxygen species(ROS) play an important role in the development of retinal diseases, especially macular degeneration. The main ROS are; peroxynitrides, superoxide radicals and hydrogen peroxides. In our study, we aimed to investigate the protective effect of before oxidative damage induced by hydrogen peroxide(H2O2) in cell culture.
Materials and Methods: Oxidative stress was induced with H2O2 in human retinal pigment epithelial(ARPE-19) cells. Oxyresveratrol was applied at 7 different concentrations before oxidative damage. Protective effects were investigated by monitoring the change in cell viability with the XTT cell proliferation assay. Caspase-3 and cell-death-detection kit were used to investigate the protective effect of oxyresveratrol and its molecular mechanism.
Results: In our study, oxyresveratrol application before oxidative damage induced by H2O2 in ARPE-19 cell line increased cell viability and showed protective activity against oxidative damage in the cell. In the findings we obtained as a result of our study; When oxyresveratrol was applied to ARPE-19 cells before the oxidative damage induced by H2O2, it reduced cell damage by about 15% at 100 µM concentration. In addition, according to cell death detection and caspase-3 results, oxyresveratrol showed protective activity against oxidative damage by reducing apoptotic cell death.
Conclusion: This in vitro study is a preliminary study for the development of the protective effect of oxyresveratrol. Oxyresveratrol can be developed as an effective therapeutic agent in the prevention of retinal diseases, especially AMD, after experimental animals and clinical studies.

Keywords

ARPE-19, Oxyresveratrol, Oxidative Stress, Age-Related Macular Degeneration

Project Number

Proje numarası: 2020.08.36.1477

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