Identification and virules of Sphaeropsis tip blight (Sphaeropsis sapinea) on Pinus spp. in Istanbul and Bursa parks

Year 2018, Volume: 31 Issue: 1, 17 - 21, 30.03.2018

Filiz Ünal , Ercan Koca Ayşe Aşkın İlker Kurbetli Kamil Sarpkaya

Abstract

Sphaeropsis sapinea tip blight is one of
the most destructive fungal diseases of pine trees in the world. It causes shoot blight, canker, collar rot, root disease
and blue stain of Pinus spp. As a result of surveys
performed in the parks, recreation areas and groves in Istanbul and Bursa
provinces in January, June and October 2015, it was observed that the agent
especially causes disease on Pinus
spp. The
disease was observed as dead, brown needles at the tips of branches, shoot
blight and cankers on pine stems and branches. Twenty eight diseased pine samples were collected from parks, recreation
areas and groves. Totally
15 Sphaeropsis sapinea isolates were
obtained. Cultural and
morphological characteristics of the isolates were determined using cultures
grown on potato dextrose agar (PDA).
Cultures on PDA were initially white
with profuse aerial mycelia that after one week turned grey to blackish. Identification
of the pathogen was based on morphological characteristics of the conidia and DNA sequence analysis. Genomic DNA was
extracted and the internal transcribed spacer (ITS) region of the rDNA was
amplified using primers ITS1 and ITS 4. Pathogenicity tests were made with twig
inoculation method. All isolates formed lesions on twigs.

Keywords

Sphaeropsis sapinea, Tip blight, Molecular, Pinus spp

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