KOLOREKTAL KANSERDE sFRP-4, TFF-3, NF-κB ve ROMO1 DÜZEYLERİNİN ROLÜNÜN ARAŞTIRILMASI: HASTALIĞIN PATOFİZYOLOJİSİ VE PROGRESYONUNA ETKİLERİ

Year 2024, , 120 - 126, 29.02.2024

Sinem Durmuş , Berrin Papila Kundaktepe , Remise Gelişgen , Çiğdem Papila , Hafize Uzun

https://doi.org/10.53446/actamednicomedia.1391225

Abstract

Amaç: Kolorektal kanser (KRK), yüksek morbidite ve mortalite oranları ile önemli bir küresel sağlık sorunudur. Erken teşhis ve doğru tanı araçları klinik seyrin yönetimi için kritik öneme sahiptir. Bu araştırma, geleneksel tanı yaklaşımlarının ötesinde bilgiler sağlamayı amaçlayarak KRK'nin moleküler manzarasını araştırmaktadır. Bu çalışmanın temel amacı, salgılanan frizzled ilişkili protein-4 (sFRP-4), trefoil faktör-3 (TFF-3), nükleer faktör-kappa-B (NF-κB) ve reaktif oksijen türleri modülatörü-1 (Romo1) gibi spesifik biyobelirteçlerin, KRK’nin patofizyolojisini anlamaya ve progresyonunu belirlemeye potansiyel katkılarını araştırmaktır.
Yöntem: Bu çalışmada, KRK'li hastalar (n=50) ile yaş ve cinsiyet açısından eşleştirilmiş kontrol grubunda (n=40) sFRP-4, TFF-3, NF-κB ve Romo1'in plazma düzeyleri ELISA yöntemi ile analiz edilmiştir. Bu biyobelirteçlerin tanısal performansı ROC analizi ile değerlendirilmiştir.
Bulgular: Araştırmamız, KRK tanısı alan hastalarda NF-κB, TFF-3 ve Romo1 seviyelerinde önemli bir artış olduğunu ortaya koymuştur. Ayrıca, bu parametrelerin 4 cm'den büyük tümörü olan hastalarda daha küçük tümörü olanlara kıyasla daha yüksek seviyelerde olduğu tespit edilmiştir. Metastazı olan hastalarda da metastazı olmayan hastalara kıyasla üç parametrenin seviyeleri de yüksek bulunmuştur. ROC analizi, NF-κB'nin hastaları kontrol bireylerinden ayırt etmek için potansiyeli en iyi biyobelirteç olabileceğini, TFF-3'ün ise tümör boyutunu ve metastaz varlığını belirlemede en fazla potansiyel gösterdiğini ortaya koymuştur.
Sonuç: Bu araştırma, KRK'nın patofizyolojisinin ve progresyonunun anlaşılmasına katkı sağlamaktadır. Çalışmamızda ortaya konduğu üzere NF-κB, TFF-3 ve Romo1'in biyobelirteç olarak potansiyel rolleri, KRK'nin erken teşhisi ve yönetimi için umut verici bir yaklaşım sunmaktadır. Bu biyobelirteçlerin KRK'nin patofizyolojik mekanizmasındaki rollerini netleştirmek ve klinik faydalarını belirlemek için daha fazla doğrulama ve ileriye dönük çalışmalar gereklidir.

Keywords

Kolorektal kanser, NF-κB, Romo1, sFRP-4, TFF-3

EXPLORING THE ROLE OF sFRP-4, TFF-3, NF-кB AND ROMO1 LEVELS IN COLORECTAL CANCER: IMPLICATIONS FOR PATHOPHYSIOLOGY AND DISEASE PROGRESSION

Abstract

Objective: Colorectal cancer (CRC) is a significant global health concern with high morbidity and mortality rates. Early detection and accurate diagnostic tools are critical for managing the clinical course. This research explores the molecular landscape of CRC, aiming to provide valuable insights beyond traditional diagnostic approaches. The main aim of this study was to investigate the potential contribution of specific biomarkers, such as secreted frizzled associated protein-4 (sFRP-4), trefoil factor-3 (TFF-3), nuclear factor-kappa-B (NF-κB) and reactive oxygen species modulator-1 (Romo1), to understanding the pathophysiology and determining the progression of CRC.
Methods: This study analyzed plasma levels of sFRP-4, TFF-3, NF-κB and Romo1 in a cohort of patients with CRC (n=50) and age- and gender-matched control group (n=40), utilizing ELISA. The diagnostic performance of these biomarkers was assessed through Receiver Operating Characteristic (ROC) analysis.
Results: Our research revealed a significant increase in the levels of NF-κB, TFF-3 and Romo1 in patients with a diagnosis of CRC. Furthermore, these parameters were found to maintain elevated levels in patients with tumors larger than 4 cm as opposed to those with smaller tumors. Patients with metastases also had elevated levels of the three parameters compared with patients without metastases. The ROC analysis revealed that NF-κB showed the most promise as a parameter for distinguishing patients from control subjects, whereas TFF-3 displayed the most potential in identifying tumor size and the presence of metastasis.
Conclusion: This research contributes valuable insights into understanding the pathophysiology and progression of CRC. The potential roles of NF-κB, TFF-3, and Romo1 as biomarkers, as revealed in our study, offer a promising avenue for early detection and improved management of CRC. Further validation and prospective studies are necessary to clarify the roles of these biomarkers in the pathophysiological mechanism of CRC and to establish their clinical utility.

Keywords

Colorectal cancer, NF-κB, Romo1, sFRP-4, TFF-3

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