Timokinon, sağlıklı sıçanlarda hipokampal miR-26b, miR-124 ve miR-29a mikroRNA'larının ekspresyonlarını etkiler

Year 2024, , 353 - 358, 27.10.2024

Ayça Doğan Mollaoğlu , Merve Beker , Tuğçe Dallı Birsen Elibol

https://doi.org/10.53446/actamednicomedia.1562729

Abstract

Amaç: Nigella sativa'nın ana biyoaktif bileşeni olan Thymoquinone (TQ), kan beyin bariyerini geçerek nöroprotektif ve nöromodülatör aktiviteler gösterir. Bu çalışmanın amacı, TQ uygulamasının sağlıklı sıçanların hipokampüsünde 26b, 124, 29a ve 29c mikroRNA'larının (miR) ekspresyonları üzerindeki etkisini araştırmaktır.
Yöntem: TQ (20 mg kg-1 d-1) yetişkin sıçanlara 15 gün boyunca intragastrik olarak uygulanmıştır. İlgili genlerin mikroRNA seviyeleri gerçek zamanlı polimeraz zincir reaksiyonu kullanılarak analiz edilmiştir.
Bulgular: TQ uygulaması miR-26b ve miR-124'ün ifade profillerini önemli ölçüde aşağı düzenlerken miR-29a'nın ifadesini yukarı düzenlemiştir. miR-29c'nin ifade düzeyinde anlamlı bir etki gözlenmemiştir.
Sonuç: TQ, beyinde yüksek oranda ifade edilen miR-26b, miR-124 ve miR-29a'nın ifadelerini değiştirerek sağlıklı beyin ve/veya merkezi sinir sistemi fonksiyonlarında faydalı bir role sahip olabilir.

Keywords

Timokinon, kara tohum, kara kimyon, mikroRNA, hipokampüs

Ethical Statement

The experimental protocol of this study was approved by the Committee for Animal Research Ethics in Bezmialem Vakif University (2015/229).

Supporting Institution

Bezmialem Vakif University

Project Number

BVU-BAP(12.2014/2)

Thanks

This study was supported by the grant from Bezmialem Vakif University Scientific Research Found (BVU-BAP) (12.2014/2).

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