The Effects of Rosemary (Rosmarinus Officinalis L.) Extract on the Proliferation and Apoptosis of A549 and H1299 Human Lung Cancer Cells

Year 2024, Volume: 7 Issue: 3, 293 - 300, 27.10.2024

Ebru Alimoğulları , Bahar Kartal , Tuba Özdemir Sancı , Sinem Aslan Erdem , Aslı Ceylan

https://doi.org/10.53446/actamednicomedia.1474338

Abstract

ABSTRACT
Objective: Apoptosis resistance and increased proliferation rates are characteristics of cancer cells. The anticancer properties of rosemary (Rosmarinus officinalis L.) extract (RE) have been demonstrated in a small number of in vivo and in vitro animal studies; however, no research has investigated the role of RE in human non-small cell lung cancer (NSCLC) A549 and H1299 cells, and its underlying mechanism of action remains unknown. In the current study, we examined the effects of RE on human non-small cell lung cancer cell proliferation, survival, and apoptosis.
Methods: Human non-small cell lung cancer (NSCLC) A549 and H1299 were incubated with (2.5 µg/ml, 5 µg/ml, 7.5 µg/ml, 10 µg/ml, and 12.5 µg/ml) doses of RE for 12, 24, and 48 hours. MTT, Annexin V-PI, and caspase 3/7 assay kit were performed to detect the cell viability and apoptosis.
Results: According to MTT analysis, the viability of A549 and H1299 human lung cancer cells was reduced by approximately 49.74% and 47.76%, respectively, for 24 hours by treatment with a dose of 5 µg/ml RE. The results of Annexin V-PI staining and Caspase 3/7 activation showed that RE had a greater effect on inducing cell death.
Conclusion: In conclusion, we can say that rosemary extract has both apoptotic and antiproliferative properties on human lung cancer cells. We might propose that additional investigation is necessary to ascertain the therapeutic impacts of rosemary extract.

Keywords

Annexin V-PI, A549, Caspase 3/7, H1299, Rosemary extract.

Biberiye (Rosmarinus Officinalis L.) Ekstresinin A549 ve H1299 İnsan Akciğer Kanseri Hücrelerinin Çoğalması ve Apoptozu Üzerindeki Etkileri

Abstract

ÖZ
Amaç: Apoptoz direnci ve artan proliferasyon oranları kanser hücrelerinin karakteristik özelliğidir. Biberiye (Rosmarinus officinalis L.) ekstresinin (RE) antikanser özellikleri, az sayıda in vivo ve in vitro hayvan çalışmasında gösterilmiştir; ancak RE'nin insandaki Küçük Hücreli Olmayan Akciğer Kanseri (NSCLC) A549 ve H1299 hücrelerindeki rolünü araştıran araştırma yoktur ve bunun altında yatan etki mekanizması belirsizliğini korumaktadır. Bu çalışmada RE'nin insandaki Küçük Hücreli Olmayan Akciğer Kanseri hücre hatlarının çoğalması, canlılığı ve apoptoz üzerindeki etkilerini araştırdık.
Yöntem: İnsandaki Küçük Hücreli Olmayan Akciğer Kanseri (NSCLC) A549 ve H1299 hücre hatları 12, 24 ve 48 saat süre boyunca (2.5 µg/ml, 5 µg/ml, 7.5 µg/ml, 10 µg/ml ve 12.5 µg/ml) RE dozlarıyla inkübe edildi. Hücre canlılığı ve apoptoz belirlemek için MTT, Annexin V-PI ve kaspaz 3/7 kiti kullanıldı.
Bulgular: MTT analizine göre, A549 ve H1299 insan akciğer kanseri hücrelerinin canlılığı, 5 µg/ml RE doz tedavisiyle 24 saat boyunca yaklaşık sırasıyla % 49,74 ve % 47,76 oranında azaldı. Annexin V-PI ve Kaspaz 3/7 aktivasyonunun sonuçları, RE'nin hücre ölümünü indüklemede büyük bir etkiye sahip olduğunu gösterdi.
Sonuç: Sonuç olarak biberiye ekstresinin insan akciğer kanseri hücreleri üzerinde hem apoptotik hem de antiproliferatif özelliklere sahip olduğunu söyleyebiliriz. Biberiye ekstresinin terapötik etkilerini belirlemek için daha fazla araştırmaya ihtiyaç vardır.

Keywords

Annexin V-PI, A549, Biberiye ekstresi, Caspase 3/7, H1299

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