The Relationship Between miR-196a2 Polymorphism and Colorectal Cancer Risk

Year 2024, Volume: 7 Issue: 3, 348 - 352, 27.10.2024

Bahar Canbay Torun , Şakir Ümit Zeybek , Türker Bulut , Yılmaz Büyükuncu , Emel Canbay

https://doi.org/10.53446/actamednicomedia.1562372

Abstract

ABSTRACT
Objective
MicroRNAs are small endogenous, non-coding, single-stranded posttranscriptional RNA molecules. The discovery of microRNAs has made new contributions to cancer diagnosis and treatment. These microRNAs reported as a responsible for colorectal cancer development with several epigenetic changes. In this study, it was aimed to evaluate the relationship between the polymorphism of miR-196a-2 polymorphism rs11614913 and colorectal cancer in Turkish population.
Methods
Two hundred colorectal cancer patient (124 colon cancer and 76 rectal cancer) and 240 health control individuals were included in our study, which was planned as a hospital based retrospective cohort study. MiR-196a2 polymorphism in peripheral blood samples has been determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. Significance of the results has been evaluated by using SPSS (20.0 SPSS Inc., Chicago, IL, USA.) statistical program.
Results
miR-196a2 C / C + C / T genotypes was found to be associated with the risk of colorectal cancer development (p: 0.001; OR: 2.04, 95% CI: 1.293-3.236). The subgroup analysis, showed that the C / C + C / T genotype increased the risk of colon cancer development 2.11 times (p: 0.016; 95% CI: 1.136-3.918) and rectal cancer 2.86 times (p: 0.011; 95% CI:1.242-6.592). The relationship between any clinicopathological features of colorectal cancer and the frequency of the C / C + C / T genotype of miR196a2 was not statistically significant (p> 0.05).
Conclusion
This study supports that miR-196a2's C / C + C / T genotypes is related with increased colorectal cancer development risk.

Keywords

Colorectal cancer, MicroRNA, miRNA-196a2 polymorphism

MiR-196a2 Polimorfizmi ile Kolorektal Kanser Riski Arasındaki İlişki

Abstract

ÖZ
Amaç
MicroRNA’lar küçük, endojen, kodlanmayan, tek sarmallı posttranskripsiyonel RNA molekülleridir. MikroRNA’ların keşfi, kanser teşhis ve tedavisine yeni katkılar sağlamıştır. Bu miRNA’ların çeşitli epigenetik değişiklerle kolorektal kanser gelişiminden sorumlu olduğu saptanmıştır. Bu çalışmada Türk toplumunda miR-196a2’nin rs11614913 polimorfizmi ile kolorektal kanser arasındaki ilişkinin değerlendirilmesi amaçlanmıştır.
Yöntem
Hastane bazlı retrospektif kohort çalşması olarak planlanan çalışmamıza 200 kolorektal kanser hastası (124 kolon kanseri ve 76 rektum kanseri) ve 240 sağlıklı kontrol bireyler dahil edilmiştir. Periferik kan örneklerinde miR-196a2 polimorfizmi, polimeraz zincir reaksiyonu ve restriksiyon fragman uzunluk polimorfizmi yöntemi ile belirlenmiştir. Sonuçların anlamlılığı SPSS (20.0 SPSS Inc., Chicago, IL, USA.) istatistik programı kullanılarak değerlendirilmiştir.
Bulgular
MiR-196a2 C/C + C/T genotiplerinin kolorektal kanser gelişim riski ile ilişkili olduğu saptanmıştır (p: 0.001; OR: 2.04, %95 CI: 1.293-3.236). Altgrup analizleri ise ise C/C+C/T genotipinin kolon kanseri gelişim riskini 2.11. kat (p: 0.016; %95 CI: 1.136-3.918) ve rektum kanserini ise 2.86 kat (p: 0.011; %95 CI: 1.242-6.592) arttırdığını göstermiştir. Kolorektal kanserin herhangi bir klinikopatolojik özelliği ile miR-196a2’nin C/C+ C/T genotipinin sıklığı arasında istatistiksel olarak anlamlı ilişki saptanmamıştır (p>0.05).
Sonuç
Bu çalışma miR-196a2'nin C / C + C / T genotiplerinin artmış kolorektal kanser gelişim riski ile ilişkili olduğunu desteklemektedir.

Keywords

Kolorektal kanser, MikroRNA, miRNA-196a2 polimorfizmi

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