Crocus cancellatus subsp. mazziaricus (Herbert) Mathew Bitki Ekstraktının Avrupa Deniz Levrek Balığı (Dicentrarchus labrax, L.1758) Doğal Bağışıklık Sistemi Üzerine Etkisi

Year 2020, Volume: 16 Issue: 1, 148 - 157, 01.03.2020

Canan Öntaş , Gülşen Uluköy , Esin Baba , Ramazan Mammadov

https://doi.org/10.22392/actaquatr.622606

Cited By: 1

Abstract

Bu çalışmada Crocus cancellatus subsp. mazziaricus (Herbert) Mathew geofit bitki ekstraktı iki farklı konsantrasyonda (0,5 ve 2 mg/balık) intraperitonal enjeksiyon yöntemiyle Avrupa deniz levreğine (Dicentrarchus labrax, L. 1758) uygulanmış ve bağışıklık sistemine etkisi araştırılmıştır. Enjeksiyon sonrası 7., 14., 21., 28. ve 35. günlerde balıklardan kan örnekleri alınmıştır. Doğal bağışıklık ile ilgili parametrelerden olan toplam lökosit sayısı, hematokrit seviye, NBT(+) hücre sayısı, lizozim aktivitesi ve serum total protein miktarındaki değişimler tespit edilmiştir. Crocus cancellatus subsp. mazziaricus uygulanan deneme gruplarında kontrol grubuna kıyasla doğal bağışıklık sisteminin aktifleşerek, NBT(+) hücre sayısının 21. günde, toplam lökosit sayısının 14.günde, serum total protein miktarının 14. ve 21. günlerde ve serum lizozim aktivitesinin 21. günde en yüksek seviyeye ulaştığı tespit edilmiştir. Sonuç olarak, 2 mg/balık dozunda uygulanan C. cancellatus subsp. mazziaricus ekstraktının 0,5 mg/balık dozuna kıyasla daha etkin olduğu bulunmuştur.

Keywords

İmmunostimulant, bitki ekstraktı, bağışıklık sistemi, Crocus cancellatus subsp. mazziaricus, Dicentrarchus labrax

Supporting Institution

tübitak

Project Number

104V126

Thanks

Bu çalışma, TUBİTAK tarafından desteklenen 104V126 no’lu kariyer projesi içerisinde gerçekleştirilmiş olup “Crocus cancellatus (Herbert) Mathew bitkisinin levrek balığının (Dicentrarchus labrax, Linnaeus 1758) spesifik olmayan immün sistem üzerine etkisinin araştırılması” isimli yüksek lisans tezi olarak savunulmuştur.

Effects of Crocus cancellatus subsp. mazziaricus (Herbert) Mathew Plant Extract on the Innate Immune System of European Sea bass (Dicentrarchus labrax, L.1758)

Abstract

In this study, Crocus cancellatus subsp. mazziaricus (Herbert) Mathew geophyte plant extract was applied to European sea bass (Dicentrarchus labrax, L. 1758) in two different concentrations (0.5 and 2 mg/fish) by intraperitoneal injection method and its effect on the immune system was investigated. After injection on the 7th, 14th, 21st, 28th and 35th days, blood samples were collected from fish. Some of the innate immune related parameters in blood and serum samples such as total leukocyte count, hematocrit level, NBT (+) cell count, lysozyme activity and serum total protein content were determined. C. cancellatus subsp. mazziaricus plant extract treated groups exhibited significant immunostimulatory effect, increased NBT (+) cell count at 21st day, total leucocyte count in 14th day, serum total protein level at 14th and 21st day and serum lysozyme activity at 21st day compared with the control group. As a result of plant extract application, the 2 mg/fish group was found to be more effective than the 0.5 mg/fish group.

Keywords

Immunostimulant, plant extract, immune system, Crocus cancellatus subsp. mazziaricus, Dicentrarchus labrax.

Project Number

104V126

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