Atık Sudan İzole Edilen Bakteri Suşlarının Biyofilm Oluşumu ve Genişletilmiş Spektrum Beta Laktamaz (ESBL) Üretimi

Year 2020, Volume: 16 Issue: 2, 170 - 178, 01.06.2020

Hatice Aysun Mercimek Takcı , Pemra Bakırhan , Sema Genç Melike Kaptanoğlu

https://doi.org/10.22392/actaquatr.622902

Abstract

Bu
çalışmada Kilis Devlet Hastanesi kanalizasyon suyundan izole edilen biyofilm
oluşturan suşların genişlemiş spektrumlu beta laktamaz ve metallo-beta laktamaz
üretimi incelenmiştir. Biyofilm üretiminin belirlenmesi için kongo kırmızısı
agar, tüp adherens, doku kültürü plak testleri ve SEM analizi uygulanmıştır.
Biyofilm üreten suşların genişlemiş spektrumlu beta laktamaz ve metallo-beta
laktamaz ekspresyonu CLSI
yönetmeliğine göre Kirby-Bauer disk difüzyon metodu kullanılarak test
edilmiştir. 5 klinik izolatın biyofilm üreticisi olduğu belirlenmiştir. Bu
izolatlardan Enterococcus spp. (C2)
ve E. coli
suşları güçlü biyofilm üretmiştir. Enterococcus
izolatlarından C1’in SEM mikrografında cam yüzeye zayıf tutunma gözlenmiştir. 5
izolattan yalnızca biri, Enterococcus spp.’nin
(C2) GSBL enzimi sentezlediği ortaya konmuştur. Suşlardan hiçbirinin metallo-beta laktamaz üretmediği
belirlenmiştir. GSBL ve biyofilm üretimi Enterococcus spp. (C2)
suşuna antibiyotik tedavilerine karşı koruyarak önemli bir patojenik karakter sağlamaktadır.

Keywords

Biyofilm, GSBL, metallo-beta laktamaz, SEM

Thanks

SEM mikrografları için Çukurova Üniversitesi Merkezi Araştırma Laboratuvarı (ÇÜMERLAB)'na teşekkürler.

Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water

Abstract

The
production extended spectrum beta lactamase (ESBL) and metallo-beta lactamase of strains grown biofilm that were isolated from
Kilis State Hospital sewage water were investigated in this study. Congo red
agar, tube adherence, tissue culture plate tests and SEM analysis were
performed for the detection of biofilm production. The extended spectrum beta lactamase and metallo-beta lactamase
expression of biofilm producing bacteria was tested by using Kirby-Bauer disc diffusion method according to CLSI
guidelines. 5 clinical isolates were revealed to be biofilm producer. From of
these isolates, Enterococcus spp. (C2)
and E. coli strains
showed strong biofilm producing. One of Enterococcus
isolates, SEM micrograph of C1 was viewed the weak adherence on glass surface. Only one from 5 isolates, Enterococcus spp. (C2)
strain was determined to synthesis ESBL enzyme. None of strains was observed to
not produce metallo-beta
lactamase. ESBL and biofilm production provide an important pathogenic
character allowing protection against antibiotic treatments to Enterococcus spp. (C2).

Keywords

Biofilm, ESBL, metallo-beta lactamase, SEM

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