Acı Kavun (Ecballıum Elaterıum L. 1758)’un Sazan (Cyprınus Carpıo L. 1758) Balıkları Kan Hücre Morfolojilerine Etkileri
Abstract
Yüzyıllardır birçok hastalığın tedavisinde modern tıp tarafından uygulanan, kanıta ve bilimsel çalışmalara dayalı tedavi seçeneklerinin yanı sıra bitkiler ve bitkisel ilaçların da kullanıldığı bilinmektedir. Tedavi amaçlı kullanılan bitkilerden bir tanesi acı kavun (Ecballium elaterium) bitkisidir. Acı kavun bitkinin tedavi edici özelliğinin yan ısıra, insanlar üzerinde ciddi biyolojik hasarlar da bıraktığı yapılan çalışmalarda görülmektedir. Bu çalışmada, acı kavun bitkisine ait olgun meyvelerden elde edilen özsuyun sazan balıkları (Cyprinus carpio)’nın hücre morfolojilerine etkisinin olup olmadığının belirlenmesi amaçlanmıştır. Uygulama 3 deney ve 1 kontrol grubu olmak üzere 4 grup üzerinde yapılmıştır. Üç farklı deney grubuna ait akvaryumlara 3 farklı ppm (100, 200 ve 300) değerinde acı kavun özsuyu 72 saat uygulanmıştır. Balıklardan kan yayma preparatları hazırlanmıştır ve hazırlanan preparatlar mikroskop altında taranarak kan hücrelerinin morfolojilerinde meydana gelen değişiklikler tespit edilmiştir. Sonuç olarak acı kavun özsuyunun C. carpio kan hücrelerinde bölünme esnasında mikronükleus oluşumuna neden olduğu ve kan hücrelerinin morfolojilerinde Loblu nükleus (LB), Tomurcuklu nükleus (BL), Çentikli nükleus (NT) ve Binükleat (BN) gibi anormalliklere neden olduğu tespit edilmiştir.
Supporting Institution
KIRŞEHİR AHİ EVRAN ÜNİVERSİTESİ BAP
Project Number
FEF.A4.18.010
References
- Aktümsek, A., & Konuk, M. (2002). Canlılar Bilimi, Nobel Yayın, 2. Baskı, Ankara.
- Baytop, T. (1982). Türkiye’de Bitkiler ile Tedavi, Nobel Tıp Kitapevleri, İstanbul.
- Carrasco, K. R., Tilbury, K.L., & Myers, M.S. (1990). Assessment of the piscine micronucleus test as an in situ biological indicator of chemical contaminant effects, Canadian Journal of Fisheries and Aquatic Sciences, 4 (7): 2123-2136.
- Claver, J. A., & Quaglia, A. I. E. (2009). Comparative Morphology, Development and Function of Blood Cells in Nonmammalian Vertebrates, Journal of Exotic Pet Medicine,18, 87–97.
- Çelik, T. A., Aslantürk, Ö. S. (2009). Investigation of Cytotoxic and Genotoxic Effects of Ecballium elaterium Juice Based on Allium Test, Methods Find Exp Clin Pharmacol, 31(9): 591-596
- Fenech, M.F., Dunaiski, V, Osborne, Y., & Morley, A.A. (1991). The Cytokinesis-Block Micronucleus Assay as a Biological Dosimeter in Spleen and Peripheral Blood Lymphocytes of the Mouse Following Acute Whole-Body Irradiation, Mutation Research, 263: 119-126.
- Fenech, M. (2000). The In Vitro Micronucleus Technique, Mutation Research 455, s.81–95.
- Fenech, M. (2007). Cytokinesis-Block Micronucleus Cytome Assay, Nature Protocols Vol.2 No.5, 1084-1105.
- Gill, T.S., & Pant, J.C. (1985). Mercury-induced blood anomalies in the freshwater teleost, Water Air Soil Pollut, 24, 165–171
- Kuru, M. (2013). Kemikli Balıklar, Omurgalı Hayvanlar, 6. Bölüm, Palme Yayıncılık, Ankara, ISBN:978-975-747-52-5, 207-208.
- Mazokopakis, E., Karefilakis, C. M., & Starakis, I. K. (2009). The Safety and Efficacy of the Fruit Juice of Ecballium elaterium in the Treatment of Acute Rhinosinusitis, The Journal of Alternative and Complementary Medicine, 2, 1273-1274.
- Mumford, S., Heidel, J., Smith, C., Morrison, J., MacConnell, B., & Blazer, B. (2007). Fish Histology and Histopathology, U.S. Fish and Wildlife Service, National Conservation Training Center.
- Örün, İ. (2000). Karakaya Baraj Gölünde Yaşayan ve Ekonomik Öneme Sahip Bazı Balık Türlerinin [Acanthobrama marmid (Heckel 1843), Leuciscus cephalus orientalis (Nordman 1840), Chondrostoma regium (Heckel 1843) ve Capotea capotea umbla (Heckel 1843)] Hematolojik Yönden İncelenmesi, İnönü Üniversitesi Fen Bilimleri Enstitüsü, Malatya, 182.
- Shabbar, M.S., & Maslat, A.O. (2007). Genotoxicity of Ecballium elaterium (L). A Rich Cucurbitaceae Fruit Juice Using Micronucleus Assay and DNA Single Strand Break Techniques, The Internet Journal of Health, Vol. 6 Nr. 2.
- Şekeroğlu, V., & Şekeroğlu, Z., A. (2011). Genotoksik Hasarın Belirlenmesinde Mikronükleus Testi, Türk Hijyen ve Deneysel Biyoloji Dergisi, 64(4), s.241-252.
- Verhovsek, M., & McFarlane, A. (2004). Abnormalities in Red Blood Cells, McKean, S.C., Ross, J.J, Dressler, D.D., Scheurer, D.B., Principles and Practice of Hospital Medicine, American College of Physicians Second Edition, 2017;1400-1419
The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758)
Abstract
It is well-known that for centuries, humans have used plants and herbal remedies to treat many diseases, in addition to the treatment options brought and applied by modern medicine, which relies on scientific studies and actual evidence. One of these plants is the squirting cucumber (Ecballium elaterium), or exploding cucumber. Despite its therapeutic properties, the squirting cucumber may also cause serious biological damage to humans, as demonstrated by a number of studies. This study was conducted with the aim of determining whether the juice obtained from ripe fruits of squirting cucumber has any effect on the cell morphology of common carp (Cyprinus carpio). Juice application was carried out on 4 groups, composed of 1 control and 3 experimental groups. Squirting cucumber juice was added to three aquariums containing different experimental groups, in three different ppm levels, for 72 hours. Afterwards, blood smear samples of the fish were prepared, and the preparations were scanned under a microscope in order to determine the changes in blood cell morphology. The results demonstrated that squirting cucumber juice caused micronucleus formation during cell division in C. carpio blood cells, and a number of abnormalities in blood cell morphology including Lobed nucleus (LB), Bud nucleus (BL), Notched nucleus (NT) and Binucleated erythrocytes (BN).
Project Number
FEF.A4.18.010
References
- Aktümsek, A., & Konuk, M. (2002). Canlılar Bilimi, Nobel Yayın, 2. Baskı, Ankara.
- Baytop, T. (1982). Türkiye’de Bitkiler ile Tedavi, Nobel Tıp Kitapevleri, İstanbul.
- Carrasco, K. R., Tilbury, K.L., & Myers, M.S. (1990). Assessment of the piscine micronucleus test as an in situ biological indicator of chemical contaminant effects, Canadian Journal of Fisheries and Aquatic Sciences, 4 (7): 2123-2136.
- Claver, J. A., & Quaglia, A. I. E. (2009). Comparative Morphology, Development and Function of Blood Cells in Nonmammalian Vertebrates, Journal of Exotic Pet Medicine,18, 87–97.
- Çelik, T. A., Aslantürk, Ö. S. (2009). Investigation of Cytotoxic and Genotoxic Effects of Ecballium elaterium Juice Based on Allium Test, Methods Find Exp Clin Pharmacol, 31(9): 591-596
- Fenech, M.F., Dunaiski, V, Osborne, Y., & Morley, A.A. (1991). The Cytokinesis-Block Micronucleus Assay as a Biological Dosimeter in Spleen and Peripheral Blood Lymphocytes of the Mouse Following Acute Whole-Body Irradiation, Mutation Research, 263: 119-126.
- Fenech, M. (2000). The In Vitro Micronucleus Technique, Mutation Research 455, s.81–95.
- Fenech, M. (2007). Cytokinesis-Block Micronucleus Cytome Assay, Nature Protocols Vol.2 No.5, 1084-1105.
- Gill, T.S., & Pant, J.C. (1985). Mercury-induced blood anomalies in the freshwater teleost, Water Air Soil Pollut, 24, 165–171
- Kuru, M. (2013). Kemikli Balıklar, Omurgalı Hayvanlar, 6. Bölüm, Palme Yayıncılık, Ankara, ISBN:978-975-747-52-5, 207-208.
- Mazokopakis, E., Karefilakis, C. M., & Starakis, I. K. (2009). The Safety and Efficacy of the Fruit Juice of Ecballium elaterium in the Treatment of Acute Rhinosinusitis, The Journal of Alternative and Complementary Medicine, 2, 1273-1274.
- Mumford, S., Heidel, J., Smith, C., Morrison, J., MacConnell, B., & Blazer, B. (2007). Fish Histology and Histopathology, U.S. Fish and Wildlife Service, National Conservation Training Center.
- Örün, İ. (2000). Karakaya Baraj Gölünde Yaşayan ve Ekonomik Öneme Sahip Bazı Balık Türlerinin [Acanthobrama marmid (Heckel 1843), Leuciscus cephalus orientalis (Nordman 1840), Chondrostoma regium (Heckel 1843) ve Capotea capotea umbla (Heckel 1843)] Hematolojik Yönden İncelenmesi, İnönü Üniversitesi Fen Bilimleri Enstitüsü, Malatya, 182.
- Shabbar, M.S., & Maslat, A.O. (2007). Genotoxicity of Ecballium elaterium (L). A Rich Cucurbitaceae Fruit Juice Using Micronucleus Assay and DNA Single Strand Break Techniques, The Internet Journal of Health, Vol. 6 Nr. 2.
- Şekeroğlu, V., & Şekeroğlu, Z., A. (2011). Genotoksik Hasarın Belirlenmesinde Mikronükleus Testi, Türk Hijyen ve Deneysel Biyoloji Dergisi, 64(4), s.241-252.
- Verhovsek, M., & McFarlane, A. (2004). Abnormalities in Red Blood Cells, McKean, S.C., Ross, J.J, Dressler, D.D., Scheurer, D.B., Principles and Practice of Hospital Medicine, American College of Physicians Second Edition, 2017;1400-1419
Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology |
| Journal Section | Research Articles |
| Authors | |
| Project Number | FEF.A4.18.010 |
| Publication Date | September 1, 2019 |
| Published in Issue | Year 2020 Volume: 16 Issue: 3 |
