Aeromonas Septicemia Infection and Histopathologic Findings in Cultured Narrow-Clawed Crayfish (Pontastacus leptodactylus, Eschscholtz, 1823)

Year 2024, Volume: 20 Issue: 2, 89 - 96, 01.06.2024

Şeydanur Kan , Pınar Yıldırım , Özlem Özmen , Seval Bahadır Koca , Ayşegül Kubilay

https://doi.org/10.22392/actaquatr.1298890

Abstract

In this study, bacterial, parasitic, and fungal analyses were carried out on 15 individuals showing disease symptoms in crayfish (Pontastacus leptodactylus). Samples were collected from internal organs for pathological examinations. As a result of the pathological examinations, no parasitic and fungal pathogens agents were found. Samplings were gathered from the hepatopancreas and streaked onto tryptic soy agar (TSA) 96 and consequently, a pure bacterial colony was obtained. As a result of the bacterial tests, the bacteria were determined to be Aeromonas sp. genus. Narrow-clawed crayfish showed 100% mortality within 21 days of disease outbreak. Histopathologically, significant changes were observed in hepatopancreas and intestinal tissues. In hepatopancreatic tissue, prominent pathological findings characterized by prominent vacuolization in the epithelium, irregular tubules, proliferation of epithelial cells, nuclear pycnosis, and inflammatory cell infiltrations were detected. The findings of this study demonstrated that Aeromonas sp. caused important pathological findings in narrow-clawed crayfish.

Keywords

Aeromonas sp., Narrow-Clawed Crayfish, İnfection, Histopathology.

Kültür Ortamında Yetiştiriciliği Yapılan Tatlısu Istakozu (Pontastacus leptodactylus, Eschscholtz, 1823)'nda Hareketli Aeromonas Septisemi ve Histopatolojik Bulgular

Abstract

Bu çalışmada yetiştiriciliği yapılan kerevitlerde (Pontastacus leptodactylus) hastalık belirtileri gösteren 15 adet bireyde bakteriyel, paraziter ve fungal incelemeler yapılmış; patolojik incelemeler için iç organlardan örnek alınmıştır. İncelemeler neticesinde paraziter ve fungal patojene rastlanılmamıştır. Kerevitlerin hepatopankreasından Triptik Soy Agar (TSA) besiyerine yapılan bakteriyolojik ekimden saf bakteri kolonisi izole edilmiştir. Yapılan testler sonucunda bakterinin Aeromonas sp. cinsine ait olduğu belirlenmiştir. Kerevitlerde hastalık çıktıktan sonraki 21 gün içinde %100 ölüm görülmüştür. Histopatolojik olarak; hepatopankreas ve bağırsak dokularında belirgin değişiklikler olduğu gözlemlenmiştir. Hepatopankreatik dokuda; epitellerde belirgin vakuolizasyon, tübüllerde düzensizlik, epitel hücrelerinde proliferasyon, nükleer piknoz ve inflamatuar hücre infiltrasyonları ile karakterize edilen belirgin patolojik bulgular tespit edilmiştir. Bu çalışmanın bulguları Aeromonas sp.’nin kerevitlerde önemli patolojik bulgular oluşturduğunu göstermiştir.

Keywords

Aeromonas sp., Kerevit, Enfeksiyon, Histopatoloji.

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