Effect of Vitamin C on Oxidant and Antioxidant Parameters in Cattle Immunized by Blackleg Vaccine

Year 2023, , 7 - 12, 26.12.2023

Ertan Doğan , Oğuz Merhan , Hale Ergin Eğritağ , Kadir Bozukluhan , Dinçer Erdağ

https://doi.org/10.53913/aduveterinary.1307606

Abstract

The goal of this study is to determine the effect of vitamin c on certain oxidant and antioxidant parameters in cattle immunized against blackleg. In the study, a total of 40 cattle aged 12 to 18 months, of different breeds and genders, were used in the Ardahan region. The cattle were divided into four equal groups. The Control group received 2 ml of saline, the Vitamin group received 5 mg/kg dose of vitamin c, the Vaccine-Vitamin group received 2 ml of blackleg vaccine and 5mg/kg vitamin c, and the Vaccine group received 2 ml of blackleg vaccine. Blood samples were taken from all animals before vaccination (day 0) and on the 2nd, 14th, and 28th days of administration. On the 2nd, 14th, and 28th days, malondialdehyde (MDA) and nitric oxide (NO) levels were substantially increased (P<0.05) in the Vaccine group compared to the Control and Vitamin groups, while superoxide dismutase (SOD) and catalase (CAT) levels were considerably decreased (P<0.05). It was noted that the amount of MDA and NO measured in the 2nd, 14th, and 28th days in the Vaccine-Vitamin group reduced compared to the Vaccine group, while the amount of SOD and CAT enhanced. The study concluded that the blackleg vaccine administered to cattle causes oxidative stress and lipid peroxidation, and that vitamin c acts as an antioxidant. Vitamin c administration with blackleg vaccine in cattle is believed to be beneficial for lowering oxidative stress.

Keywords

Blackleg vaccine, Cattle, Lipid Peroxidation, Oxidative stress, Vitamin C

Yanıkara Aşısı ile Aşılanan Sığırlarda C Vitamininin Oksidan ve Antioksidan Parametreler Üzerine Etkisi

Abstract

Bu araştırmanın amacı Yanıkara aşısı ile aşılanan sığırlarda c vitamininin bazı oksidan ve antioksidan parametreler üzerine etkisini belirlemektir. Araştırmada, Ardahan yöresinde ırk ve cinsiyetleri farklı, 12-18 aylık toplam 40 sığır kullanıldı. Sığırlar dört eşit gruba ayrıldı. Kontrol grubuna 2 ml serum fizyolojik, Vitamin grubuna 5 mg/kg dozda c vitamini, Aşı-Vitamin grubuna 2 ml yanıkara aşısı ve 5mg/kg c vitamini, Aşı grubuna ise 2 ml yanıkara aşısı enjekte edildi. Aşı ve ilaç uygulaması öncesinde (0.gün) ve uygulamanın 2. 14. ve 28. gününde tüm hayvanlardan kan örnekleri alındı. Uygulamanın 2.14. ve 28. gününde Aşı grubunda malondialdehit (MDA) ve nitrik oksit (NO) düzeyinin Kontrol ve Vitamin grubuna göre anlamlı oranda (P<0.05) arttığı, süperoksit dismutaz (SOD) ve katalaz (CAT) düzeylerinin ise anlamlı oranda (P<0.05) azaldığı görüldü. Aşı-Vitamin grubunda 2.14. ve 28. gün ölçülen MDA ve NO miktarının Aşı grubuna göre düştüğü, SOD ve CAT miktarının ise yükseldiği görüldü. Bu araştırmada, sığırlara uygulanan yanıkara aşısının oksidatif strese ve lipid peroksidasyonuna neden olduğu ve c vitamininin antioksidan etki gösterdiği sonucuna varıldı. Sığırlarda yanıkara aşısı ile birlikte c vitamini uygulamasının oksidatif stresi azaltma açısından faydalı olacağı düşünülmektedir.

Keywords

C vitamini, Lipid Peroksidasyonu, Oksidatif stres, Sığır, Yanıkara aşısı

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