Antiviral RNA Interference and Its Comparison with CRISPR/Cas Mechanism, A Novel Gene Editing Method

Year 2015, Volume: 4 Issue: 2, 429 - 434, 01.06.2015

B. Taylan Koç , T. Çiğdem Oğuzoğlu

Abstract

Background: RNA Interference RNAi mechanism is assumed among the most important biotechnological methods, that allows to edit the genome in the last two decades. The experiments have been performed through by this method in many sub-fields of biotechnology, and quite a lot of data were obtained. A mechanism, similar of RNAi, was discovered just then obtained data was going to evaluate as therapeutics. This mechanism that provided immunity to viruses and phages was named as CRISPR. To possess an advantage of the new method, compared to RNAi in some circumstances, is nowadays come into prominence significantly. Conclusion: RNAi, being an important tool in struggling with viruses, which forms the basis of this review and newer mechanism CRISPR and its differences are subjected in. At the end of the review, it was concluded that both methods, showing the interaction between themselves, would be impact on biotechnology branches in the near future.

Keywords

RNAi, CRISPR, Virus, Genome Editing.

Antiviral RNA İnterferenz RNAi ve Yeni Bir Gen Düzenleme Metodu CRISPR/Cas Mekanizması ile Karşılaştırılması

Abstract

Özbilgi: RNA İnterferenz RNAi mekanizması son 20 yılda genom üzerinde değişiklik yapmayı sağlayan en önemli biyoteknolojik yöntemdir. Bu metot ile biyoteknolojinin birçok alt dalında denemeler yapılmış ve oldukça fazla veri elde edilmiştir. Tam da bu verilerin terapötik olarak değerleneceği sırada yine RNAi’e benzer bir mekanizma keşfedilmiştir. Virus ve fajlara karşı bakterilerde savunma sağlayan bu mekanizmaya CRISPR adı verilmiştir. Bu yeni teknolojinin RNAi’e göre bazı durumlarda avantajı olması günümüzde onu oldukça öne çıkarmıştır. Sonuç: Özellikle viruslara karşı mücadelede önemli silah olan RNAi bu derlemenin temelini oluştururken, daha yeni olan CRISPR mekanizması ve farklılıkları da bu derlemede konu edilmiştir. Derleme sonunda karşılıklı etkileşim gösteren bu iki yöntemin yakın gelecekte biyoteknoloji alanına damga vurucağı sonucuna varılmıştır.

Keywords

RNAi, CRISPR, Virus, Genom Düzenleme.

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