Promiscuous Arylsulfatase Activity in Chlamydomonas reinhardtii

Year 2022, Volume: 19 Issue: 2, 213 - 218, 30.12.2022

Münevver Aksoy

https://doi.org/10.25308/aduziraat.1103450

Cited By: 1

Abstract

Sulfate is an essential macroelement for all living organisms. However, sulfate can be limited in agricultural settings. Microorganisms change their gene expression to acclimate to sulfate deficient conditions. Green microalga Chlamydomonas reinhardtii expresses and secretes extracellular arylsulfatase (ARS) under sulfate deficient conditions. Our results show that C. reinhardtii ARS can hydrolyze both sulfate monoester (5-bromo-4-chloro-3 indolyl sulfate; X-SO42-) and phosphate monoester (5-bromo-4-chloro-3 indolyl phosphate; X-PO43-) providing evidence that ARS enzyme has promiscuous activity. C. reinhardtii is found in soil and fresh water habitats in nature. This promiscuous activity can be beneficial in making both sulfate and phosphate bioavailable for uptake by soil organisms and plant roots.

Keywords

Chlamydomonas reinhardtii, Arylsulfatase, Sulfate

Supporting Institution

Akdeniz University BAP Commission

Project Number

FBA-2018-4156

Chlamydomonas reinhardtii'de Seçici Olmayan Arilsülfataz Aktivitesi

Abstract

Sülfat canlılar için zorunlu bir makroelementtir. Buna rağmen, sülfat tarımsal alanlarda eksik olabilir. Mikroorganizmalar gen ekspresyonlarını değistirerek sülfat eksikliğine aklimasyon gösterirler. Yeşil mikroalg Chlamydomonas reinhardtii sülfat eksikliği altında arilsülfataz (ARS) sentezler ve hücre dışına salgılar. Bu çalışmadaki sonuçlarımız, C. reinhardtii ARS’ın hem sülfat monoesterlerini (5-bromo-4-chloro-3 indolyl sulfate; X-SO42-) hem de fosfat monoesterlerini (5-bromo-4-chloro-3 indolyl phosphate; X-PO43-) hidrolize ettiğini göstermiştir. Bu da ARS’ın seçici olmayan aktiviteye sahip olduğuna kanıt sunmaktadır. C. reinhardtii toprak ve taze su ortamlarında yaşamaktadır. Sahip olduğu seçici olmayan ARS aktivitesi hem sülfatın hem de fosfatın bitki tarafından kullanılabilir forma getirilmesine katkı sağlayabilir.

Keywords

Chlaydomonas reinhardtii, Arilsülfataz, Sülfat, Seçici olmayan enzim

Project Number

FBA-2018-4156

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