Liriomyza sativae Blanchard, 1938 (Diptera: Agromyzidae)’nin DNA Barkodlaması

Year 2023, Volume: 20 Issue: 1, 167 - 172, 30.06.2023

Cem Dayan , Eyüp Mennan Yıldırım

https://doi.org/10.25308/aduziraat.1296979

Abstract

Bu çalışmada İncirliova ve Koçarlı ilçelerinde (Aydın) sebze alanlarından toplanmış Liriomyza sativae’ nin DNA barkodlaması 2020-2022 yılları arasında yapılmıştır. Morfolojik olarak tür teşhisi yapıldıktan sonra; örneklerin COI gen bölgesinin çoğaltılması için HCO/ LCO evrensel primerler kullanılmış ve 612 bç COI bölgesi elde edilmiştir. Örneklerin sekans analizi Triogen Biyoteknoloji (İstanbul-Türkiye) tarafından yapılmıştır. Örnekler NCBI’da bulunan BLAST aracılığıyla kontrol edildiğinde türlerin L. sativae (% 99-100) ve L. trifolii (%97-99) olduğu görülmüştür. Örnekler Genbank’a kaydedilmiştir. Çalışma sonucunda genetik çeşitlilik düşük bulunmuştur.

Keywords

COI, DNA barkodlama, Liriomyza sativae, Tür tanımlama

Supporting Institution

Aydın Adnan Menderes Üniversitesi Bap Birimi

Project Number

ZRF-20004

Thanks

Dr. Öğr.Üyesi Ferhat KİREMİT ve Prof. Dr. Hasan Sungur CİVELEK'e teşekkür ederiz.

DNA Barcoding of Liriomyza Sativae Blanchard Agromyzidae, 1938 (Diptera: Agromyzidae

Abstract

In this study, DNA barcoding of Liriomyza sativae collected from vegetable areas in Incirliova and Koçarlı districts (Aydın) was performed between 2020-2022. After the morphological diagnosis of L. sativae, HCO/LCO universal primers were used to reproduce the COI gene region of the samples and 612 bp COI regions were obtained. The sequence analysis of the samples was performed by Triogen Biotechnology (Istanbul, Türkiye). When the samples were checked through the BLAST located at NCBI, it was found that the species were L. sativae (99-100%) and L. trifolii (97-99%). The samples were registered to Genbank. As a result of the study, genetic diversity was found to be low.

Keywords

COI, DNA barcoding, Liriomyza. sativae, identification

Project Number

ZRF-20004

References

• Amin S, Scheffer SJ, Lewis ML, Pasha MK, Bhuiya BA (2014) DNA barcoding of the vegetableleafminer Liriomyza sativae Blanchard (Diptera: Agromyzidae) in Bangladesh. DNA Barcodes, 2: 29-33.
• Andersen A, Nordhus E, Vu TTA, Ha QH, Hofsvang T (2002) Polyphagaous Liriomyza species (Diptera: Agromyzidae) in vegetables in Vietnam. Tropical Agriculture, 79: 241-246.
• Bhuiya BA, Amin S, Mazumdar S (2011) First report of vegetable Leafminer Liriomyza sativae Blanchard (Diptera: Agromyzidae) through DNA barcoding. From Bangladesh. Journal of Taxonomy & Biodiversity Research, 5: 15-17.
• Civelek HS, Önder F (1997) Bitki hastalık etmenlerinin taşınmasında galerisineklerinin (Diptera: Agromyzidae) rolü üzerinde bir inceleme. Türkiye Entomoloji Dergisi, 3: 233-241.
• Civelek HS, Ulusoy MR (2000) Türkiye galerisinekleri (Diptera: Agromyzidae) için yeni bir kayıt: Ophiomyia phaseoli (Tryon, 1895). Türkiye Entomoloji Dergisi, 24: 163-166.
• Civelek HS, Tonguç A, Özgül O, Dursun O (2007) Contributions to The Turkish (Diptera: Agromyzidae) Fauna from Anatolian Part of Turkey, with sixteen New Records Mitteilungen Des Internationalen Entomologischen Vereins, 32(3-4): 151- 160.
• Civelek HS (2002) New records for the Turkish (Diptera: Agromyzidae) from Mugla Province, Western Turkey. Insecta Mundi, 16: 49-55
• Civelek HS (2003) Checklist of Agromyzidae (Diptera) Family of Turkey, with a New Record. Phytoparasitica. 31:132-138.
• Civelek HS (2004) Two new records for the Turkish Agromyzidae (Diptera) fauna. Türkiye Entomoloji Dergisi, 28: 1-10.
• Costa AS, Silva DM, Duffus JE (1988) Plant virus transmission by leafminer fly.Virology, 5(1): 145- 149 Çıkman E, Uygun N (2003) The determination of leafminers (Diptera:Agromyzidae) from Turkey, with four new records. Phytoparasitica, 33(4): 391-396.
• Çıkman E, Civelek HS (2005) Contributions to the Leafminer Fauna (Diptera: Agromyzidae) from Turkey, with Four New Records. Phytoparasitica, 33(4): 391-396.
• Çıkman E, Civelek HS (2007) Does Liriomyza cicerina Affect the Yield of Chickpeas (Cicer arietinum), Phytoparasitica, 35(2): 116-118.
• Deeming JC, Civelek HS (1997) Türkiye Agromyzidae (Diptera) familyası için yeni kayıtlar. Türkiye 3. Entomoloji Kongresi Bildirileri, Türkiye, Ankara, 526-533.
• Felsenstein J (1985) Confidence limits on the phylogenies an approach using the bootstrap. Evolution, 39(4): 783-791.
• Ferreira ECB, Freitas MTDS, Sombra KDDS, Siqueira HÁAD, Araujo ELD, Balbino VDQ (2017) Molecular Identification of Liriomyza sp. in the Northeast and Southeast Regions of Brazil. Revista Caatinga, 30(4): 892-900.
• Giray H (1970) Liriomyza cicerina Rond. (Diptera: Agromyzidae)’ nın morfolojik karakterleri, kısa biyolojisi ve zarar şekli üzerinde araştırmalar. Ege Üniversitesi Ziraat Fakültesi Dergisi No:171, Bornova, 34s.
• Liang YX, Du SJ, Zhong YJ, Wang QJ, Zhou Q, Wan FH, Guo JY, Liu WX (2023) Molecular phylogeny and identification of Agromyzid leafminers in China, with a focus on the worldwide genus Liriomyza (Diptera: Agromyzidae). Journal of Integrative Agriculture. https://www.sciencedirect.com/science/article/pii/S2095311923001004 (Erişim tarihi: Mayıs 2023).
• Librado P, Rozas J (2009) DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 25: 1451-1452.
• Mart C, Tursun AÖ, Civelek HS (2005) Contributions to (Diptera: Agromyzidae) Fauna of Turkey. Journal of Turkish Zoology, 29(3): 57–359.
• Nakamura S, Masuda T, Mochizuki A, Konishi K, Tokumaru S, Ueno K, Yamaguch T (2013) Primer design for identifying economically important Liriomyza species (Diptera: Agromyzidae) by multiplex PCR. Molecular Ecology Resources, 13(1): 96-102.
• Polat F, Serkan DE, Bingöl G, Kekillioğlu A (2018) Kocaeli’de Yayılış Gösteren Bazı Böcek Türlerinin Mitokondrial Sitokrom Oksidaz Alt Ünite 1 Geni ile Filogenetik Analizi. Kocaeli Üniversitesi Fen Bilimleri Dergisi, 1(2): 62-66.
• Spencer KA (1972) Agromyzidae from Southern Spain (Insecta: Diptera). Zoological Museum University of Copenhagen, 2(6): 91–104.
• Spencer KA (1973) Diptera:Agromyzidae of economic importance. The Pitman Press, G.Britain, 418 s.
• Spencer KA (1976) The (Diptera: Agromyzidae) of Fennoscandia and Denmark. Fauna Entomologica Scandinavica, 5(1–2): 1–606.
• Spencer KA (1990) Host specialization in the world (Diptera: Agromyzidae). Kluver Academic Publishers, Netherland, 444 s.
• Scheffer SJ, Lewis ML, Joshi RC (2006) DNA Barcoding Applied to Invasive Leafminers (Diptera: Agromyzidae) in the Philippines, Annual Entomological Society of America. 99(2): 204- 210.
• Tang XT, Ji Y, Chang YW, Shen Y (2016) Population genetic structure and migration patterns of Liriomyza sativae in China: moderate subdivision and no Bridgehead effeckt revealed by microsatellites.
• Tajima F (1989) Statistical methods to test for nucleotide mutation hypothesis by DNA polymorphism. Genetics, 123: 585-595.
• Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10(3): 512-526.
• Uygun N, Polatöz Z, Başpınar H (1995) Doğu Akdeniz Bölgesi Agromyzidae (Diptera) familyası türleri üzerinde sistematik araştırmalar. Türkiye Entomoloji Dergisi, 19(2): 123-136.
• Yıldırım EM, Ünay A, Civelek HS (2010) The effect of Liriomyza trifolii (Burgess) (Diptera: Agromyzidae) on some leaf characteristics of bean (Phaseolus vulgaris L.). Journal of Food, Agriculture Environment, 8(3&4): 839-841.
• Wang J, Qiao GX (2009) DNA barcoding of genus Toxoptera Koch (Hemiptera: Aphididae)identification and molecular phylogeny inferred from mitochondrial COI sequences. Insect Science, 16(6): 475-584.