Aydın Yöresinde Yabani Hardal (Sinapis arvensis L.), Laden (Cistus criticus) Ve Karabaş (Lavandula stoechas) Alanlarında Üretilen Arı Polenlerinin Tek Bitki Poleni Olarak Değerlendirilmesi ve Bazı Kimyasal Özelliklerinin Belirlenmesi

Year 2024, Volume: 21 Issue: 1, 69 - 76, 30.06.2024

Mehmet Yağcıoğlu Aytül Uçak Koç

https://doi.org/10.25308/aduziraat.1435984

Abstract

Bu çalışmada, yörede yaygın olarak bulunan yabani hardal, laden ve karabaş otu alanlarından belirli dönemlerde hasat edilen polenlerin tek bitki poleni olarak üretilebilme olanakları değerlendirilmiş ve bu bitkilerden toplanan arı polenlerinde bazı kimyasal özellikler saptanmıştır. Araştırmada, Aydın-Efeler’de yabani hardal ve Aydın ilinin Koçarlı ilçesine bağlı Gaffarlar köyünde laden ve karabaş otu alanlarına 4’er koloni taşınmıştır. Çalışmada, renklerine göre ayrılmış her gruptan alınan polen peletlerinin bitki orjinleri saptanmıştır. Bitki orjini belirlenen yabani hardal, laden ve karabaş otu polen peletlerinden alınan örneklerde pelet ağırlıkları, nem içerikleri, ham protein ve toplam fenolik madde miktarı belirlenmiştir. Yabani hardal, laden ve karabaş otu pelet ağırlıkları sırasıyla; 10,42±0,440 mg, 11,69±0,393 mg ve 10,40±0,34 mg olarak belirlenmiştir. Nem içeriği yabani hardal (%22,10±0,934), laden (%19,82±0,613) ve karabaş otu (%20,55±0,667), ham protein içeriği yabani hardal (%20,5±0,47), laden (%13,8±2,51) ve karabaş otu (%16,5±0,95) ve toplam fenolik madde değerleri yabani hardal, laden ve karabaş otunda sırasıyla; 21,53±0,514 mg GAE/g, 16,12±0,539 mg GAE/g ve 20,31±0,677 mg GAE/g olarak saptanmıştır. Bu çalışma ile Aydın yöresinde yabani hardal ve laden alanlarında üretilecek arı polenlerinin monofloral arı poleni olarak değerlendirilebileceği saptanmıştır. Laden arı poleninin ham protein içeriği ve toplam fenolik madde içeriğinin diğer iki bitkiye göre daha düşük bulunmuştur. Farklı yörelerden toplanacak daha çok sayıdaki yabani hardal ve laden arı polen örneklerinde yapılacak daha kapsamlı kimyasal analizler sonucu bu arı polenlerine ait standart oluşturmak mümkün olacaktır.

Keywords

Bal arısı, tek bitki poleni, toplam fenolik madde, polen peleti

Supporting Institution

ADÜ BAP

Evaluation of Bee Pollen Produced in Wild Mustard (Sinapis arvensis L.), Rock Rose (Cistus criticus) and Wild Lavender (Lavandula Stoechas) Fields in Aydın Vicinity as Monofloral Bee Pollen and Determining Some Chemical Properties

Abstract

In this study, the possibility of producing pollens harvested in certain periods from wild mustard (WM), rock rose (RR) and wild lavender (WL) fields, which are common in the region, as single plant pollen was evaluated and some chemical properties were determined in bee pollen collected from these plants. In the research, 4 colonies each were moved to WM fields in Aydın-Efeler, and to the RR and WL fields in Gaffarlar village of Koçarlı district of Aydın province. In these colonies, the plant origins of the pollen pellets taken from each group, separated according to their colors, were determined. Pellet weights, moisture contents, crude protein and total phenolic substance amounts were determined in the samples taken from WM, RR and WL pollen pellets of defined plant origin. WM, RR and WL pellet weights were 10,42±0,440 mg, 11,69±0,393 mg and 10,40±0,34 mg, respectively. In the same order as the moisture content were determined as 22,10±0,934%, 19,82±0,613%, 20,55±0,667%. In this study, the crude protein contents of WM, RR and WL bee pollen are; 20,5±0,47%; 13,8±2,51%; 16,5±0,95% also in the same order; total phenolic substance values were determined as 21,53±0,514 mg GAE/g, 16,12±0,539 mg GAE/g and 20,31±0,677 mg GAE/g. With this study, it was relize that the bee pollen produced in WM and RR fields in the Aydın precinct can be considered as monofloral bee pollen. Crude protein content and total phenolic substance content of RR bee pollen were found to be lower than the other two plants. It will be possible to establish standards with more comprehensive chemical analyzes on more WM and RR bee pollen samples collected from different vicinity.

Keywords

Honey bee, Monofloral Pollen, Total Phenolic Content, Pollen Pellet

Supporting Institution

ADÜ BAP

Thanks

We would like to thank Aydın Adnan Menderes University Scientific Research Projects Coordination Office for providing financial support to our project. (Project ID No. ZRF-22023).

References

• Alimoglu G, Guzelmeric E, Yuksel PI, Celik C, Deniz I, Yesilada E (2021). Monofloral and polyfloral bee pollens: Comparative evaluation of their phenolics and bioactivity profiles. Lwt,142,110973.
• Almeida-Muradiana LB, Pamplonaa LC, Coimbraa S, Barth OM (2005). Chemical composition and botanical evaluation of dried bee pollen pellets. Journal of Food Composition and Analysis, 18:105–111.
• Baloğlu GH, Gurel F (2015). The effects of pollen protein content on colony development of the bumblebee, Bombus terrestris L. Journal of Apicultural Science, 59(1), 83–88.
• Barth O, Freitas A, Oliveira E, Silva R, Maester F, Andrella RS, Cardozo GBQ (2010). Evaluation of thebotanical origin of commercial dry bee pollen load batches using pollen analysis: a proposal for technical standardization. Anaisda Academia Brasileirade Ciencias, 82(4):893–902.
• Baydar H, Gürel F (1998). Antalya doğal florasında bal arısı (Apis mellifera)’ nın polen toplama aktivitesi, polen tercihi ve farklı polen tiplerinin morfolojik ve kalite özellikleri. Journal of Agriculture and Forestry, 22:475–482.
• Bleha R, Shevtsova T, Kruzik V, Brindza J, Sinica A (2019). Morphology, physicochemical properties and antioxidant capacity of bee pollens. Czech Journal of Food Sciences, 37(1), 1-8.
• Bogdanov S (2016). Pollen: Collection, Harvest, Composition, Quality, The Pollen Book, Bee Product Science, www.bee-hexagon.net
• Campos MG, Bogdanov S, de Almeida-Muradian LB, Szczesna T, Mancebo Y, Frigerio C, Ferreira F (2008). Pollen composition and standardisation of analytical methods. Journal of Apicultural Research, 47, 154–161.
• Crane E (1990). Bees and Beekeeping: Science, Practice and World Resources. Heinemann Newnes Oxford, UK. Pp.xvii+614pp
• De Melo AAM, Estevinho LM, Moreira MM, Delerue-Matos C, de Freitas ADS, Barth OM, de Almeida-Muradian LB (2018). A multivariate approach based on physicochemical parameters and biological potential for the botanical and geographical discrimination of Brazilian bee pollen. Food Bioscience, 25, 91–110.
• Deveci M, Cınbırtoğlu Ş, Demirkol G (2016). Arı yetiştiriciliğinde ballıbaba (Lamium purpureum L.) bitkisi ve poleni. Küresel Mühendislik Çalışmaları Dergisi, 3(2): 16–20.
• Dominguez-Valhondo D, Gonzalez-Gomez D, Hernandez-Mendez T, Bohoyo-Gil D (2013). Influence of the industrial processing and the floral origin into the volatile constituents of honeybee collected pollen. Food Science and Technology International, 19 (2): 167–176.
• Freitas ASD, Arruda VASD, Muradian LB, Barth OM (2013). The botanical profiles of dried bee pollen loads collected by Apis mellifera (Linnaeus) in Brazil.
• Fuenmayor BC, Zuluaga DC, Diaz MC, Quicazan de CM, Cosio M, Mannino S (2014). Evaluation of the physicochemical and functional properties of Colombian bee pollen. Revista MVZ Cordoba, 19(1), 4003–4014.
• Gösterit A, Gürel F (2014). Bombus arısı (Bombus terrestris L.)’ nın ticari yetiştiriciliği için temel gereklilikler. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 9 (2):102–111.
• Karaca A, Kösoğlu M, Boz Ö (2006). Aydın ili Çine-Karpuzlu yöresinde balarılarının nektar ve poleninden faydalanabileceği bitkiler. ADÜ Ziraat Fakültesi Dergisi; 3(1): 21–26.
• Kalaycıoğlu Z, Kaygusuz H, Döker S, Kolaylı S, Erim FB (2017). Characterization of Turkish honeybee pollens by principal component analysis based on their individual organic acids, sugars, minerals, and antioxidant activities. LWT, 84, 402–408.
• Keskin M, Özkök A (2020). Effects of drying techniques on chemical composition and volatile constituents of bee pollen. Czech Journal of Food Sciences, 38(4), 203–208.
• Kroyer G, Hegedus N (2001). Evaluation of bioactive properties of pollen extracts as functional dietary food supplement. Innovative Food Science & Emerging Technologies, 2(3), 171–174.
• LeBlanc BW, Davis OK, Boue S, DeLucca A, Deeby T (2009). Antioxidant activity of Sonoran Desert bee pollen, Food Chemistry 115:1299–1305.
• Leja M, Mareczek A, Wyżgolik G, Klepacz-Baniak J., and Czekonska K (2007). Antioxidative properties of bee pollen in selected plant species. Food Chemistry, 100(1), 237–240.
• Lihong C (2009). Advances in propolis research and propolis industry in China. J. Royal Inst Thailand 1: 136–151.
• Lieux MH (1972). A melissopalynological study of 54 Louisianan (USA) honeys. Review Palaebotany and Palynology, (13) pp. 95-124, Amsterdam.
• Liolios V, Tananaki C, Dimou M, Kanelis D, Goras G, Karazafiris E, Thrasyvoulou A (2015). Ranking pollen from bee plants according to their protein contribution to honey bees. Journal of Apicultural Research, 54(5), 582–592.
• Magalhaes PJ, Vieira JS, Gonçalves LM, Pacheco JG, Guido LF, Barros AA (2010). Isolation of phenolic compounds from hop extracts using polyvinylpolypyrrolidone: Characterization by high-performance liquid chromatography–diode array detection–electrospray tandem mass spectrometry. Journal of Chromatography A, 1217(19), 3258-3268.
• Mahfouz HM (2016). Studies on seasonal variation of pollen collected by honeybee in North Sinai Governorate. Journal of Plant Protection and Pathology, 7(9), 565–571.
• Morais M, Moreira L, Feas X, Estevinho LM (2011). Honeybee-collected pollen from five Portuguese Natural Parks: Palynological origin, phenolic content, antioxidant properties and antimicrobial activity. Food Chemical. Toxicology, 39, 1096–1101.
• Mosic M, Trifkovic J, Vovk I, Gasic U, Tesic Z, Sikoparija B, Milojkovic-Opsenica D (2019). Phenolic composition influences the health-promoting potential of bee-pollen. Biomolecules, 9(12), 783.
• Rabie AL, Wells JD, Dent LK (1983). The nitrogen content of pollen protein. Journal of Apicultural Research, 22(2), 119–123.
• Rzepecka-Stojko A, Stec M, Kurzeja E, Gawronska E, Pawlowska-Goral K (2012). The effect of storage of bee pollen extracts on polyphenol content. Polish Journal of Environmental Studies, 21 (4): 1007–1011.
• Singleton VL, Rossi JA (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144–158.
• Sorkun K, İnceoğlu Ö (1984). İç Anadolu Bölgesi ballarında polen analizi. Doğa Bilim Dergisi, (8)2:222–228. Spulber R, Doğaroğlu M, Babeanu N, Popa O (2018). Physicochemical characteristics of fresh bee pollen from different botanical origins. Romanian Biotechnol. Lett, 23, 13357–13365.
• Tasei JN, Aupinel P (2008). Nutritive value of 15 single pollens and pollen mixes tested on larvae produced by bumblebee workers (Bombus terrestris, Hymenoptera: Apidae). Apidologie, 39(4), 397–409.
• Thakur M, Nanda V (2020). Exploring the physical, functional, thermal, and textural properties of bee pollen from different botanical origins of India. Journal of Food Process Engineering, 43(1), 1-14.
• Ulusoy E, Kolayli S (2014). Phenolic composition and antioxidant properties of Anzer bee pollen. Journal of Food Biochemistry, 38(1), 73–82.
• Villanueva MTO, Marquina AD, Serrano RB, Abellan GB (2002). The importance of bee-collected pollen in the diet: a study of its composition. International Journal of Food Sciences and Nutrition, 53: 217–224.