Sıçanlarda Siklofosfamid'in Neden Olduğu Miyelosupresyon ve Oksidatif Stres: Selenyumun Koruyucu Rolü

Year 2019, Volume: 9 Issue: 2, 252 - 265, 30.12.2019

Adnan Ayhancı , Nilhan Heybeli İlknur Kulcanay Sahin Mustafa Cengiz

https://doi.org/10.37094/adyujsci.617016

Cited By: 1

Abstract

Bu çalışmanın amacı, selenyumun (Se), CP kaynaklı hematoksisite modelinde kemik iliği ve kan toksisitesine karşı koruyucu rolünü tespit etmekti. Her grupta sıçanları 7 hayvandan oluşan 6 gruba ayırdık (kontrol; 150 mg/kg CP; 0,5 mg/kg Se; 1 mg/kg Se; 150+0,5 mg/kg CP+Se; 150+1 mg/kg). CP+Se). Se enjeksiyonları, CP enjeksiyonlarından 5 gün önce başlamış ve Se ile birlikte CP enjekte edilen gruplar için deneyin sonuna (6. gün) kadar devam etmiştir. CP anestezi öncesi tek doz olarak uygulandı. Bu nedenle 7. günde kardiyak delinme ile kan alındı ve femur yıkanarak kemik iliği alındı. Periferik kan hücreleri ve kemik iliği çekirdekli hücreler, bir hücre sayacında sayıldı. İntraperitoneal CP enjeksiyonunun lökosit sayısını %317, trombositi %36 ve kemik iliği çekirdekli hücreleri %481 azalttığı bulundu. CP' nin 0,5 ve 1 mg/kg Se'den sonra verildiği gruplarda, sadece CP verilen gruba kıyasla lökosit, trombosit ve kemik iliği çekirdekli hücrelerin sayısı önemli ölçüde artmıştır (p<0.001). Sonuçlar, 1 mg/kg Se'nin, CP ile ilişkili hematoksisite ve miyelosupresyona karşı 0,5 mg / kg'dan daha iyi bir korumaya sahip olduğunu göstermektedir. Sonuçlarımız ayrıca, Se dozlarının daha güçlü bir koruyucu etki elde etmek için CP dozundaki artışa göre ayarlanabileceği anlamına gelir. CP'nin neden olduğu hematoksisiteye karşı farklı Se dozlarının kullanılacağı konusunda daha fazla çalışmaya ihtiyaç olduğuna inanıyoruz. Se, CP kaynaklı miyelosupresyon ve lipit peroksidasyonuna karşı koruma sağlayabilir.

Keywords

Siklofosfamid, Hematoksisite, Selenyum, Miyelosupresyon, Lipit peroksidasyonu

Supporting Institution

Eskişehir Osmangazi üniversitesi BAP

Project Number

201130D05

Thanks

Eskişehir Osmangazi Üniversitesi BAP Birimine desteklerinden dolayı teşekkür ederiz.

Myelosuppression and Oxidative Stress Induced by Cyclophosphamide in Rats: The Protective Role of Selenium

Abstract

Aim of this study is to detect the protective role of selenium (Se) against bone marrow and blood toxicity in CP-induced hematoxicity model. We categorized the rats into 6 groups of 7 animals in each group (control; 150 mg/kg CP; 0.5 mg/kg Se; 1 mg/kg Se; 150+0.5 mg/kg CP+Se; 150+1 mg/kg CP+Se). Se injections started 5 days before the CP injections and carried on until the end of the experiment (6^th day) for the groups to which CP was injected together with Se. CP was applied as a single dose before anesthesia. For that reason, on the 7^th day, blood was taken with cardiac puncture and bone marrow was taken by flushing the femur. Peripheral blood cells and bone marrow nucleated cells were counted on a cell counter. Intraperitoneal CP injection was found to reduce the number of leukocytes by 317%, thrombocyte by 36% and bone marrow nucleated cells by 481% compared to the control group. In the groups where CP was given after 0.5 and 1 mg/kg Se, numbers of leukocyte, thrombocyte and bone marrow nucleated cells were considerably improved compared to the group to which CP was given only (p<0.001). Results show that 1 mg/kg Se has a better protection than 0.5 mg/kg against CP associated hematoxicity and myelosuppression. Our results also imply that the doses of Se could be adjusted according to enhance in CP dose so as to gain a stronger protective effect. We believe there is a need of further studies in which different doses of Se will be used against CP induced hematoxicity. Se can provide protection against CP-induced myelosupression and lipid peroxidation.

Keywords

Cyclophosphamide, hematoxicity, selenium, myelosupression, lipid peroxidation

Project Number

201130D05

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