The Effect of Intermittent Fasting Diet and Light-Intensity Physical Activity on Serum Irisin Levels in Elderly Individuals

Year 2024, Volume: 8 Issue: 1, 77 - 84, 29.04.2024

Hüsniye Bilge Sertdemir , Safiye Beyza Dursun , Shaafah Namulodı , Mehmet Salih Kaya , Farhri Bayıroğlu

https://doi.org/10.46332/aemj.1331280

Abstract

Purpose: The aim of this study was to evaluate the effects of exercise and intermittent fasting diet on some anthropometric measurements and circulating irisin concentration and to investigate the relationship between irisin and body fat percentage in elderly subjects.

Materials and Methods: Forty-four elderly volunteers (21 men and 23 women) with a mean age of 69±9 years were randomly divided into 4 groups; Control (n=8), Bocce group (n=12), Intermittent fasting group (n=12), Bocce and Intermittent fasting group (n=12). Those in the control group continued with their normal activities. The Bocce group played Bocce for two hours five days a week. Those in the intermittent fasting group followed a 16-hour food restriction schedule on two non-consecutive days of the week. In addition to the Bocce Game, participants in the Intermittent Fasting and Bocce groups were given a food restriction regimen. The interventions were sustained for 12 weeks.

Results: There was no significant difference in serum irisin levels between the same groups before and after treatment (p>0.05). Body fat percentage decreased significantly in all groups except the increase in the control group (p<0.05). Body weight decreased significantly in Bo and IF groups (p<0.05). Although there was a significant decrease (p<0.05) in body fat percentage in the IF group compared to the control group, the similar significant difference observed in irisin levels was probably not the result of the experimental treatment, but was probably due to the inevitable heterogeneous distribution in terms of age between the groups. There was no correlation between body fat percentage and body weight change and circulating irisin levels (r=0.116, p=0.437; r=-0.145, p=0.649, respectively).

Conclusion: Intermittent fasting and Bocce intervention resulted in a decrease in body fat percentage and weight in elderly subjects, but not in serum irisin levels. This may be due to the slower response time to irisin in elderly individuals and/or the duration and intensity of exercise.

Keywords

aged, bocce game, irisin, intermittent fasting

Aralıklı Açlık ve Hafif Yoğunluklu Fiziksel Aktivitenin Yaşlı Bireylerde Serum İrisin Düzeylerine Etkisi

Abstract

Amaç: Bu çalışmanın amacı, yaşlı bireylerde egzersiz ve aralıklı açlık diyetinin bazı antropometrik ölçümler ve dolaşımdaki irisin konsantrasyonu üzerindeki etkilerini değerlendirmek ve irisin ile vücut yağ yüzdesi arasındaki ilişkiyi araştırmaktı.

Araçlar ve Yöntem: Yaş ortalaması 69±9 olan 44 yaşlı gönüllü (21 erkek ve 23 kadın) rastgele 4 gruba ayrıldı; Kontrol (n=8), Bocce grubu (n=12), Aralıklı açlık grubu (n=12), Bocce ve Aralıklı açlık grubu (n=12). Kontrol grubundakiler günlük rutinlerine devam etti. Bocce grubundakiler haftanın 5 günü 2 saat boyunca Bocce Oyunu oynadı. Aralıklı açlık grubundakilere ardışık olmayan haftanın iki günü 16 saatlik besin kısıtlaması programı uygulandı. Bocce ve Aralıklı açlık grubundakilere Bocce Oyununa ilaveten besin kısıtlaması programı uygulandı. Uygulamalara 12 hafta devam edildi.

Bulgular: Uygulama öncesi ve sonrası aynı gruplar karşılaştırıldığında serum irisin değerleri arasında anlamlı bir fark bulunmadı (p>0.05). Vücut yağ yüzdesi, kontrol grubundaki artış dışında diğer tüm gruplarda anlamlı olarak azaldı (p<0.05). Vücut ağırlığı Bo ve IF gruplarında önemli ölçüde azaldı (p<0.05). Uygulama sonrası gruplar kontrol grubu ile karşılaştırıldığında, IF grubunun vücut yağ yüzdesinde anlamlı azalma (p<0.05) görülmesine rağmen, irisin seviyelerinde gözlenen benzer anlamlı farklılık muhtemelen deneysel uygulamanın sonucu olmayıp, gruplar arasındaki yaş yönünden gelişen kaçınılmaz heterojen dağılımdan kaynaklanmaktaydı. Vücut yağ yüzdesi ve vücut ağırlığı değişimi ile dolaşımdaki irisin seviyeleri arasında korelasyon bulunmadı (sırasıyla r=0.116, p=0.437; r=-0.145, p=0.649).

Sonuç: Aralıklı açlık ve Bocce müdahalesi yaşlı bireylerde vücut yağ yüzdesi ve ağırlığında azalmaya sebep olurken, serum irisin değerlerinde anlamlı bir değişikliğe yol açmadı. Bu durum, yaşlı bireylerde irisine verilen cevap süresinin daha yavaş olmasından ve/veya egzersizin süre ve yoğunluğundan kaynaklanıyor olabilir.

Keywords

aralıklı açlık, bocce oyunu, irisin, yaşlı

References

• 1. M, Oxlund B, Jespersen A, et al. The challenges of human population aging. Age Ageing. 2015;44(2):185-187.
• 2. Bouchard C, Blair SN, Katzmarzyk PT. Less Sitting, More Physical Activity, or Higher Fitness? Mayo Clin Proc. 2015;90(11): 1533-1540.
• 3. Cartee GD, Hepple RT, Bamman MM, Zierath JR. Exercise Promotes Healthy Aging of Skeletal Muscle. Cell Metab. 2016;23(6):1034-1047.
• 4. Machado SA, Pasquarelli-do-Nascimento G, da Silva DS, et al. Browning of the white adipose tissue regulation: new insights into nutritional and metabolic relevance in health and diseases. Nutr Metab (Lond). 2022; 19(1):61-61.
• 5. Domaszewski P, Konieczny M, Pakosz P, Bączkowicz D, Sadowska-Krępa E. Effect of a Six-Week Intermittent Fasting Intervention Program on the Composition of the Human Body in Women over 60 Years of Age. Int J Environ Res Public Health. 2020;17(11):4138.
• 6. Hatori M, Vollmers C, Zarrinpar A, et al. Time-restricted feeding without reducing caloric intake prevents metabolic diseases in mice fed a high-fat diet. Cell Metab. 2012; 15(6):848-860.
• 7. Boström P, Wu J, Jedrychowski MP, et al. A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;481(7382):463-468.
• 8. Aydin S. Three new players in energy regulation: Preptin, adropin and irisin. Peptides. 2014;56:94-110.
• 9. Maak S, Norheim F, Drevon CA, Erickson HP. Progress and Challenges in the Biology of FNDC5 and Irisin. Endocr Rev. 2021;42(4):436-456.
• 10. Perakakis N, Triantafyllou GA, Fernández-Real JM, et al. Physiology and role of irisin in glucose homeostasis. Nat. Rev. Endocrinol. 2017;13(6):324-337.
• 11. Von Haehling S, Morley JE, Anker SD. An overview of sarcopenia: facts and numbers on prevalence and clinical impact. J Cachexia Sarcopenia Muscle. 2010;1(2): 129-133.
• 12. Kim HJ, Lee HJ, So B, Son JS, Yoon D, Song W. Effect of Aerobic Training and Resistance Training on Circulating Irisin Level and Their Association With Change of Body Composition in Overweight/Obese Adults: a Pilot Study. Physiol Res. 2016;65(2):271-279.
• 13. Morelli C, Avolio E, Galluccio A, et al. Impact of Vigorous-Intensity Physical Activity on Body Composition Parameters, Lipid Profile Markers, and Irisin Levels in Adolescents: A Cross-Sectional Study. Nutrients. 2020;12(3):742.
• 14. Tsuchiya Y, Ando D, Goto K, Kiuchi M, Yamakita M, Koyama K. High-Intensity Exercise Causes Greater Irisin Response Compared with Low-Intensity Exercise under Similar Energy Consumption. Tohoku J. Exp. Med. 2014;233(2):135-140.
• 15. Karras SN, Koufakis T, Adamidou L, et al. Effects of Christian Orthodox Fasting Versus Time-Restricted Eating on Plasma Irisin Concentrations Among Overweight Metabolically Healthy Individuals. Nutrients. 2021;13(4):1071.
• 16. Alzoughool F, Al Hourani H, Atoum M, Abdelgader R, Alanagreh L. Irisin, leptin and adiponectin levels are reduced significantly during fasting. Med J Nutrition Metab. 2019;12(4):389-396.
• 17. De Macêdo SM, Lelis DdF, Mendes KL, et al. Effects of Dietary Macronutrient Composition on FNDC5 and Irisin in Mice Skeletal Muscle. Metab Syndr Relat Disord. 2017;15(4):161-169.
• 18. Osella AR, Colaianni G, Correale M, et al. Irisin Serum Levels in Metabolic Syndrome Patients Treated with Three Different Diets: A Post-Hoc Analysis from a Randomized Controlled Clinical Trial. Nutrients. 2018;10(7):844.
• 19. Ko B-J, Park KH, Shin S, et al. Diet quality and diet patterns in relation to circulating cardiometabolic biomarkers. Clin Nutr. (Edinburgh, Scotland). 2016;35(2):484-490.
• 20. Qiu S, Cai X, Sun Z, Schumann U, Zügel M, Steinacker JM. Chronic Exercise Training and Circulating Irisin in Adults: A Meta-Analysis. Sports Med. 2015;45(11):1577-1588.
• 21. Kim H-j, So B, Choi M, Kang D, Song W. Resistance exercise training increases the expression of irisin concomitant with improvement of muscle function in aging mice and humans. Exp Gerontol. 2015;70:11-17.
• 22. Wu J, Boström P, Sparks LM, et al. Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell. 2012;150(2):366-376.
• 23. Zhao J, Su Z, Qu C, Dong Y. Effects of 12 Weeks Resistance Training on Serum Irisin in Older Male Adults. Front Physiol. 2017;8:171.
• 24. Cosio PL, Crespo-Posadas M, Velarde-Sotres Á, Pelaez M. Effect of Chronic Resistance Training on Circulating Irisin: Systematic Review and Meta-Analysis of Randomized Controlled Trials. Int J Environ Res Public Health. 2021;18(5):2476.
• 25. Fox J, Rioux BV, Goulet EDB, et al. Effect of an acute exercise bout on immediate post‐exercise irisin concentration in adults: A meta‐analysis. Scand J Med Sci Sports. 2017;28(1):16-28.
• 26. Huh JY, Panagiotou G, Mougios V, et al. FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. mRNA expression and circulating concentrations in response to weight loss and exercise. Metabolism. 2012;61(12):1725-1738.
• 27. Peterson JM, Mart R, Bond CE. Effect of obesity and exercise on the expression of the novel myokines, Myonectin and Fibronectin type III domain containing 5. PeerJ. 2014;2:e605.
• 28. Norheim F, Langleite TM, Hjorth M, et al. The effects of acute and chronic exercise on PGC-1α, irisin and browning of subcutaneous adipose tissue in humans. FEBS J. 2013;281(3):739-749.
• 29. Besse-Patin A, Montastier E, Vinel C, et al. Effect of endurance training on skeletal muscle myokine expression in obese men: identification of apelin as a novel myokine. Int J Obes. 2013;38(5):707-713.