Lavandula × intermedia’da Terpen Biyosentezi ile İlgili Genlerin Tanımlanması ve Moleküler Karakterizasyonu

Year 2023, Volume: 54 Issue: 3 - Research in Agricultural Sciences, 103 - 112, 19.10.2023

Damla Önder , Sercan Önder , Yaşar Karakurt

https://doi.org/10.5152/AUAF.2023.23150

Abstract

Lavandin (Lavandula × intermedia), içerdiği koku bileşenleri nedeniyle kozmetik ve ilaç endüstrileri için ekonomik değeri yüksek olan bir uçucu yağ bitkisidir. Bu uçucu yağlar esas olarak geraniol, linalool ve linalil asetat dahil olmak üzere monoterpenler tarafından düzenlenir. Lavandinin uçucu yağ miktarı L. angustifolia’dan beş kat daha fazladır. Buna rağmen en kaliteli ve en çok talep edilen lavanta yağı nispeten yüksek linalool ve linalil asetat içeriği nedeniyle L. angustifolia’dan elde edilmektedir. Bu çalışmada, lavandin çiçeklerinin glandüler trikomlarından geranil difosfat sentaz (GPPS), linalool sentaz (LIS), linalil asetil transferaz (LAT) genlerini kodlayan genler izole edilmiştir. Sonuçlar LiGPP (L. × intermedia geranil difosfat sentaz), LiLIS (L. × intermedia linalool sentaz) ve LiLAT (L. × intermedia linalil asetil transferaz) genlerinin tomurcuklanmadan çiçek gelişiminin tam açma aşamasına kadar ifade edildiğini ve LiLIS geninin ifadesinin LiGPP ve LiLAT ifadelerine kıyasla daha düşük olduğunu göstermiştir. Filogenetik analizler, LiLIS geninin L. angustifolia’daki LIS geni ile benzer seviyelerde olduğunu, LiGPP ve LiLAT genlerinin ise diğer lavander türlerindeki GPP ve LAT genlerinden farklı seviyede bulunduğunu göstermiştir. Lavandinde terpen sentaz genlerinin tanımlanması, metabolik mühendislik yoluyla lavandindeki uçucu yağ bileşiminin kalitesinin
iyileştirilmesi için temel oluşturma potansiyeline sahiptir.

Keywords

Uçucu yağ, gen klonlama ve ifadesi, geranil difosfat sentaz, lavandin, linalool sentaz, linalil asetil transferaz

Identification and Molecular Characterization of Genes Related to Terpene Biosynthesis From Lavandula × intermedia

Abstract

Lavandin (Lavandula × intermedia Emeric ex Loisel.) is an essential oil-bearing plant with high economic value for the cosmetics and pharmaceutical industries due to its fragrance components. These essential oils primarily contain monoterpenes, including geraniol, linalool, and linalyl acetate. The essential oil content of Lavandula × intermedia is five times higher than that of Lavandula angustifolia oil. However, due to its relatively high content of linalool and linalyl acetate, the highest quality and sought-after lavender oil is produced from Lavandula angustifolia. In this study, the genes encoding geranyl diphosphate synthase, linalool synthase, and linalyl acetyl transferase were isolated from glandular trichomes of lavandin flowers. The results showed that Lavandula × intermediageranyl diphosphate synthase, Lavandula × intermedia linalool synthase, and Lavandula × intermedia linalyl acetyl transferase demonstrated expressions from the budding to the full blooming stage of flower development, and the expression levels of Lavandula × intermedia linalool synthase were lower in comparison with the expressions of Lavandula × intermedia geranyl diphosphate synthase and Lavandula × intermedia linalyl acetyl transferase. Phylogenetic analyses showed that Lavandula × intermedia linalool synthase and Lavandula angustifolia linalool synthase were at similar levels, while Lavandula × intermedia geranyl diphosphate synthase, Lavandula × intermedia linalyl acetyl transferase, and other lavender species geranyl diphosphate and linalyl acetyl transferase were different. The identification of these terpene synthases from lavandin forms the basis for improving the quality of essential oil composition in lavandin through metabolic engineering.

Keywords

Essential oil, gene cloning and expression, geranyl diphosphate synthase, lavandin, linalool synthase, linalyl acetyl transferase

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