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İNCE BAĞIRSAK KARBONİK ANHİDRAZ AKTİVİTESİ ÜZERİNE ORNİDAZOL VE METRONİDAZOL İLAÇLARININİN VİTROETKİSİ

Year 2017, Volume: 1 Issue: 1, 1 - 13, 01.01.2017

Fatma Ergün , Dilek Dülger Nazan Demir

Abstract

Bu araştırmada sığır ince bağırsağından total olaraksaflaştırılan karbonik anhidraz EC 4.2.1.1. enzimi aktivitesiüzerine bazı ilaçlarınBağırsakafinitekromatografisi ile saflaştırıldı. Saf olarak elde edilmişolan karbonik anhidraz enziminin aktivitesi üzerine ornidazolve metronidazol etken maddesine sahip ilaçların etkileriaraştırıldı. Aktivite ölçümlerinde karbonik anhidrazın CO2hidrataz aktivitesinden yararlanıldı. 3 farklı konsantrasyondave 6 farklı hacimde yapılan ölçümlerin sonuçları Aktivite-[İlaçhacmi] şeklinde grafik halinde verildi. Elde edilen bulgulardan,ornidazol ve metronidazol ilaçlarının bağırsak CA enzimaktivitesini aktive ettiği belirlendi

Keywords

İnce bağırsak Karbonik anhidraz Ornidazol Metronidazol

References

  • Altıntop, M.D.,Ozdemir, A., Kucukoglu, K., Turan-Zitouni, G., Nadaroglu, H., Kaplancıklı, Z.A.(2015). Synthesisand Evaluation of New ThiadiazoleDerivatives as PotentialInhibitors of Human CarbonicAnhydraseIsozymes (hCA-I andhCA-II), Journal of EnzymeInhibitionandMedicinalChemistry, 30(1), 32-37. http://dx.doi.org/10.3109/14756366.2013.873038
  • Altıntop, M.D., Sever, B., Çiftçi, G.A., Kucukoglu, K., Ozdemir, A., Soleimani, S.S., Nadaroglu, H., Kaplancıklı, Z.A.(2017a).SynthesisandEvaluation of New Benzodioxole-BasedDithiocarbamateDerivatives as PotentialAnticancerAgentsandhCA-I andhCA-II İnhibitors. EuropeanJournal of MedicinalChemistry, 125, 190-196.https://doi.org/10.1016/j.ejmech.2016.09.035
  • Altıntop, M.D., Sever, B., Özdemir, A., Kucukoglu, K., Onem, H.,Nadaroglu, H.,Kaplancıklı, Z.A.(2017b).Potentialinhibitors of humancarbonicanhydraseisozymes I and II: Design, synthesisanddockingstudies of new1,3,4-thiadiazole derivatives, Bioorganic&MedicinalChemistry, 25(13), 3547-3554. doi:http://dx.doi.org/10.1016/j.bmc.2017.05.005.
  • Anderson, A.K. (2005). AffectiveInfluences on theAttentional Dynamics SupportingAwareness. Journal of ExperimentalPsychology: General, 154, 258–281. doi:10.1037/0096- 3445.134.2.258
  • Arslan, O., Nalbantoğlu, B., Demir, N., Özdemir, H., Küfrevioğlu, Ö.İ. (1996) A New MethodforthePurification at CarbonicAnhydraseIsozymesbyAffinityChromatography. Turk J MedSci 26: 163-166
  • Bradford, M.M. (1976). A rapidandsensitivemethodforthequantitation of microgramquantities of protein utilizingtheprinciple of protein-dyebinding. Anal Biochem 72: 248-254. doi.org/10.1016/0003-2697(76)90527-3
  • Britta, K.,Ralf, R. (1997). β-lactamantibioticsinhibitchloroplastdivision in a moss (Physcomitrellapatens) but not in tomato (Lycopersiconesculentum): Journal of PlantPhysiology, 150, 137- 140.https://doi.org/10.1016/S0176-1617(97)80193-9
  • Demir, Y.,Nadaroğlu, H., Demir, N. 2004. Effects of omeprazole, famotidine, andranitidine on theenzymeactivities of carbonicanhydrasefrombovinestomach in vitroandraterythrocytes in vivo, BiologicalandPharmaceuticalBulletin, 27, 1730-1734. http://doi.org/10.1248/bpb.27.1730
  • Demir, Y.,Nadaroğlu, H., Demir, N.(2006) Effect of Glimepiride on Paraoxonase Activity: PharmaceuticalBiology, 44 (5), 396–399. http://dx.doi.org/10.1080/13880200600751717
  • Ekinci, D.,Beydemir, S., Alim, Z. 2007. Somedrugsinhibit in vitrohydrataseandesteraseactivities of humancarbonicanhydrase-I and II, PharmacologicalReports, 59, 580-587.
  • Fleming, R.E.,Parkkila, S., Parkkila, A.K., Rajaniemi, H., Waheed, A., Sly, W.S. (1995) Carbonicanhydrase IV expression in ratandhumangastrointestinaltrackregional, cellularandsubcellularlocalization. J. Chin. İnvest. 96, 2907-2913.doi: 10.1172/JCI118362
  • Hansson, H.P.J. (1967) HistokimyasalDemonstration of CarbonicAnhydrase Activity. Histochemie. 11, 112-128.
  • Hewett-Emmett, D. (2000) Inthecarbonicanhtdrase- newhorizons. BirkhauserVerlag, Basel, 29-78.
  • Kohn, J.,Wilchek, M. (1978) Colorimetricmethodformonitoringactivation of sepharosebycynogenbromide. Biochem. Biophys. Res. Commun. 7, 7-14. https://doi.org/10.1016/0006-291X(78)90255-3
  • Löfmark, S.,Edlund, C. andNord, C.E. Metronidazole Is StilltheDrug of ChoiceforTreatment of AnaerobicInfectionsClinicalInfectiousDiseases 2010; 50:S16–23 doi:https://doi.org/10.1086/647939
  • Maren TH (1967) Carbonic Anhydrase, Chemistry, Physiology and Inhibition. Physiological McIntosh, J.E.A. (1969) Carbonikanhydraseisoenzymes in theerythrocytesanddorsdateralprostate of rat. Biochem. J. 114, 463-467.
  • Onat, T.,Emerk, K. (1997) Temel Biyokimya, Saray Medikal Yayıncılık, (2. Baskı) İzmir.
  • Pocker, Y.,Sarkanen, S. (1978) Carbonicanhydrase: Structure, catalyticversalityandinhibition. Adv. Enzym. 49, 149-274.
  • Rickli, E.E., Ghazanfar, S.A.S., Gibbons, B.H. and Edsall, J.T (1964) Carbonic Anhydrases from Human Erythrocytes. Journal of Biological Chemistry 239: 1065-1078
  • Sly, S.W., Peigi, Y.H. (1995) Human carbonic anhydrase and carbonic andrase deficiencies. Annu. Rev. Biochem., 64, 375-401. https://doi.org/10.1146/annurev.bi.64.070195.002111
  • Whitney, P.L.,Briggle, T.V (1982) Membrane-associatedcarbonicanhydrasepurifiedfrombovinelun g. J. Biol. Chem. 257, 12056-12059.
  • Zhu, X.L.,Sly, W.S. (1990) Carbonicanhydrase IV fromhumanlung. Purification, characterizationandcomrarisonwithmembranecarbonicandydra sefromhumankidney. J. Biol. Chem. 265, 8795-8801.

Year 2017, Volume: 1 Issue: 1, 1 - 13, 01.01.2017

Fatma Ergün , Dilek Dülger Nazan Demir

Abstract

In this study, the effects of certain drugs on the activity ofcarbonic anhydrase EC 4.2.1.1. , Which is totally purifiedfrom bovine small intestine, were investigated in vitro. Theintestinal tissue was purified by affinity chromatography ofthe carbonic anhydrase enzyme. On the activity of the purelyobtained carbonic anhydrase enzyme, the effects of drugswith ornidazole and metronidazole were investigated.Carbonic anhydrase CO2-hydratase activity was utilized inthe activity measurements. The results of the measurementsmade at 3 different concentrations and 6 different volumeswere graphically displayed in the form of activity- [drugvolume]. From the results obtained, it was determined thatornidazole and metronidazole drugs activated the intestinalenzyme activity

Keywords

Small intestine Carbonic anhydrase Ornidazole

References

  • Altıntop, M.D.,Ozdemir, A., Kucukoglu, K., Turan-Zitouni, G., Nadaroglu, H., Kaplancıklı, Z.A.(2015). Synthesisand Evaluation of New ThiadiazoleDerivatives as PotentialInhibitors of Human CarbonicAnhydraseIsozymes (hCA-I andhCA-II), Journal of EnzymeInhibitionandMedicinalChemistry, 30(1), 32-37. http://dx.doi.org/10.3109/14756366.2013.873038
  • Altıntop, M.D., Sever, B., Çiftçi, G.A., Kucukoglu, K., Ozdemir, A., Soleimani, S.S., Nadaroglu, H., Kaplancıklı, Z.A.(2017a).SynthesisandEvaluation of New Benzodioxole-BasedDithiocarbamateDerivatives as PotentialAnticancerAgentsandhCA-I andhCA-II İnhibitors. EuropeanJournal of MedicinalChemistry, 125, 190-196.https://doi.org/10.1016/j.ejmech.2016.09.035
  • Altıntop, M.D., Sever, B., Özdemir, A., Kucukoglu, K., Onem, H.,Nadaroglu, H.,Kaplancıklı, Z.A.(2017b).Potentialinhibitors of humancarbonicanhydraseisozymes I and II: Design, synthesisanddockingstudies of new1,3,4-thiadiazole derivatives, Bioorganic&MedicinalChemistry, 25(13), 3547-3554. doi:http://dx.doi.org/10.1016/j.bmc.2017.05.005.
  • Anderson, A.K. (2005). AffectiveInfluences on theAttentional Dynamics SupportingAwareness. Journal of ExperimentalPsychology: General, 154, 258–281. doi:10.1037/0096- 3445.134.2.258
  • Arslan, O., Nalbantoğlu, B., Demir, N., Özdemir, H., Küfrevioğlu, Ö.İ. (1996) A New MethodforthePurification at CarbonicAnhydraseIsozymesbyAffinityChromatography. Turk J MedSci 26: 163-166
  • Bradford, M.M. (1976). A rapidandsensitivemethodforthequantitation of microgramquantities of protein utilizingtheprinciple of protein-dyebinding. Anal Biochem 72: 248-254. doi.org/10.1016/0003-2697(76)90527-3
  • Britta, K.,Ralf, R. (1997). β-lactamantibioticsinhibitchloroplastdivision in a moss (Physcomitrellapatens) but not in tomato (Lycopersiconesculentum): Journal of PlantPhysiology, 150, 137- 140.https://doi.org/10.1016/S0176-1617(97)80193-9
  • Demir, Y.,Nadaroğlu, H., Demir, N. 2004. Effects of omeprazole, famotidine, andranitidine on theenzymeactivities of carbonicanhydrasefrombovinestomach in vitroandraterythrocytes in vivo, BiologicalandPharmaceuticalBulletin, 27, 1730-1734. http://doi.org/10.1248/bpb.27.1730
  • Demir, Y.,Nadaroğlu, H., Demir, N.(2006) Effect of Glimepiride on Paraoxonase Activity: PharmaceuticalBiology, 44 (5), 396–399. http://dx.doi.org/10.1080/13880200600751717
  • Ekinci, D.,Beydemir, S., Alim, Z. 2007. Somedrugsinhibit in vitrohydrataseandesteraseactivities of humancarbonicanhydrase-I and II, PharmacologicalReports, 59, 580-587.
  • Fleming, R.E.,Parkkila, S., Parkkila, A.K., Rajaniemi, H., Waheed, A., Sly, W.S. (1995) Carbonicanhydrase IV expression in ratandhumangastrointestinaltrackregional, cellularandsubcellularlocalization. J. Chin. İnvest. 96, 2907-2913.doi: 10.1172/JCI118362
  • Hansson, H.P.J. (1967) HistokimyasalDemonstration of CarbonicAnhydrase Activity. Histochemie. 11, 112-128.
  • Hewett-Emmett, D. (2000) Inthecarbonicanhtdrase- newhorizons. BirkhauserVerlag, Basel, 29-78.
  • Kohn, J.,Wilchek, M. (1978) Colorimetricmethodformonitoringactivation of sepharosebycynogenbromide. Biochem. Biophys. Res. Commun. 7, 7-14. https://doi.org/10.1016/0006-291X(78)90255-3
  • Löfmark, S.,Edlund, C. andNord, C.E. Metronidazole Is StilltheDrug of ChoiceforTreatment of AnaerobicInfectionsClinicalInfectiousDiseases 2010; 50:S16–23 doi:https://doi.org/10.1086/647939
  • Maren TH (1967) Carbonic Anhydrase, Chemistry, Physiology and Inhibition. Physiological McIntosh, J.E.A. (1969) Carbonikanhydraseisoenzymes in theerythrocytesanddorsdateralprostate of rat. Biochem. J. 114, 463-467.
  • Onat, T.,Emerk, K. (1997) Temel Biyokimya, Saray Medikal Yayıncılık, (2. Baskı) İzmir.
  • Pocker, Y.,Sarkanen, S. (1978) Carbonicanhydrase: Structure, catalyticversalityandinhibition. Adv. Enzym. 49, 149-274.
  • Rickli, E.E., Ghazanfar, S.A.S., Gibbons, B.H. and Edsall, J.T (1964) Carbonic Anhydrases from Human Erythrocytes. Journal of Biological Chemistry 239: 1065-1078
  • Sly, S.W., Peigi, Y.H. (1995) Human carbonic anhydrase and carbonic andrase deficiencies. Annu. Rev. Biochem., 64, 375-401. https://doi.org/10.1146/annurev.bi.64.070195.002111
  • Whitney, P.L.,Briggle, T.V (1982) Membrane-associatedcarbonicanhydrasepurifiedfrombovinelun g. J. Biol. Chem. 257, 12056-12059.
  • Zhu, X.L.,Sly, W.S. (1990) Carbonicanhydrase IV fromhumanlung. Purification, characterizationandcomrarisonwithmembranecarbonicandydra sefromhumankidney. J. Biol. Chem. 265, 8795-8801.
There are 22 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Fatma Ergün

Dilek Dülger This is me

Nazan Demir This is me

Publication Date January 1, 2017
Published in Issue Year 2017 Volume: 1 Issue: 1

Cite

APA Ergün, F., Dülger, D., & Demir, N. (2017). İNCE BAĞIRSAK KARBONİK ANHİDRAZ AKTİVİTESİ ÜZERİNE ORNİDAZOL VE METRONİDAZOL İLAÇLARININİN VİTROETKİSİ. Kırşehir Ahi Evran Üniversitesi Sağlık Bilimleri Dergisi, 1(1), 1-13.

The journal is indexed in Sobiad Citation Index, Turkish Education Index and Turkey Citation Index.