Gıdalarda Doğal Olarak Oluşan Nitrat ve Nitrit: Kapiler Elektroforez ile Anadolu Arı Polenlerindeki Tayini

Year 2021, , 139 - 148, 30.06.2021

Zeynep Kalaycıoğlu , Hatice Sena Karadeniz , Bedia Berker

https://doi.org/10.53600/ajesa.930917

Cited By: 2

Abstract

Bu çalışmanın amacı, Anadolu'nun farklı bölgelerinden toplanan bir seri arı poleninde nitrat ve nitritin kapiler elektroforetik bir teknik kullanılarak bir arada tayin edilmesidir. Ayırma ortamı olarak 30 mM formik asit / format tamponu (pH: 4.0) kullanılmıştır. Ön çalışmalarda polen örneklerinde yalnızca nitrat anyonu tespit edilmiştir. Polen numunelerinde her iki anyonun deteksiyon hassasiyetini artırmak için bir örnek sıkıştırma yöntemi uygulanmıştır. Örnek sıkıştırma yönteminde, ayırma tamponunun iletkenliği numune bölgesinden daha yüksek olmalıdır. Bu çalışmada, ayırma tamponunun iletkenliği, 30 mM sodyum sülfat ilavesiyle artırılmıştır. Enjeksiyon koşulu, 50 mbar'da 160 saniyedir. Kalibrasyon eğrileri, nitrat ve nitrit iyonları için sırasıyla 0.989 ve 0.999 korelasyon katsayıları ile 1.5-30 μmol / L ve 1.5-10 μmol / L arasında doğrusallık göstermiştir. Tüm polen örneklerinin önemli miktarda nitrat içerdiği görülmüştür (2.880 ± 0.055 - 18.72 ± 0.457 mg/kg). Nitrit içeren örneklerdeki nitrit seviyeleri ise 0.048 ± 0.008 ile 0.301 ± 0.001 mg/kg arasındadır.

Keywords

Kapiler elektroforez, Polen, Nitrat, Nitrit, Örnek sıkıştırma

Naturally Occuring Nitrate and Nitrite in Nutrients: Determination in Anatolian Honey-bee Pollens by Capillary Electrophoresis

Abstract

The aim of this work is the simultaneous determination of nitrate and nitrite in a set of bee-pollens of Anatolia using a capillary electrophoretic technique. Separation and determination conditions were 30 mM formic acid/formate buffer at pH 4.0. In earlier steps with pollen samples, only nitrate anion was detected. Consequently, a sample stacking method was applied to increase the detection sensitivity of both anions for the pollen samples. In a stacking operation, the conductivity of separation buffer should be higher than the sample zone. The conductivity of the separation buffer was increased by the addition of 30 mM sodium sulfate. Injection condition was 160 s at 50 mbar. The calibration curves show linear dynamic ranges from 1.5 to 30 μmol/L and 1.5 to 10 μmol/L with correlation coefficients of 0.989 and 0.999 for nitrate and nitrite ions, respectively. All the pollen samples contained appreciable amounts of nitrate (from 2.880 ± 0.055 to 18.72 ± 0.457 mg/kg). Low levels of nitrite were found in all samples (from 0.048 ± 0.008 to 0.301 ± 0.001 mg/kg).

Keywords

Capillary electrophoresis, Pollen, Nitrate, Nitrite, Sample stacking

Supporting Institution

İstanbul Teknik Üniversitesi Bilimsel Araştırma Projeleri Birimi

Thanks

The authors would like to thank Prof. Dr. Sevgi Kolaylı for supplying the pollen samples.

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