Simultaneous Optimization of Protease and Biopesticide Productions: A Case Study with Industrial Perspective

Year 2017, Volume: 15 Issue: 4, 327 - 336, 25.12.2017

Ahmet Katı Özlem Aytekin , Ali Özhan Aytekin , Fikrettin Şahin

https://doi.org/10.24323/akademik-gida.370086

Abstract

Bacillus sphaericus is a biopesticide
that is highly effective for the control of mosquitos under harsh conditions
such as polluted areas and UV light. In addition to its bioactivity, it
produces proteases. In this present study, the composition of media comprising
corn steep liquor (CSL) and molasses was optimised using response surface
methodology (RSM) with a central composite design (CCD). Four different
scenarios were arranged involving sole biopesticide and protease optimization
(Scenarios 1 and 2), biopesticide optimization with protease as a by-product
(Scenario 3) and protease optimization with biopesticide as a by-product
(Scenario 4). The optimization of the simultaneous production of biopesticide
and protease was not satisfactory to obtain large amounts of both products;
however, when the production aim was a sole production with a by-product,
optimal working conditions could be achieved. Also, according to industrial
view, Scenario 1 is the only possible process for large scale systems.

Keywords

Bacillus sphaericus, Biopesticide, Protease, Response surface methodology (RSM), Molasses, Corn steep liquor (CSL)

Proteaz ve Biyopestisit Üretimlerinin Eşzamanlı Optimizasyonu: Endüstriyel Bakış Açısında Bir Durum Çalışması

Abstract

Bacillus sphaericus biyopestisit olup, kirli
alanlar ve UV ışık altı gibi zorlu koşullarda bile böcekler üzerinde yüksek
etkiye sahiptir. Bu biyoaktivitesiyle beraber, proteaz enzimi de üretir. Bu
çalışmada, mısır ıslatma şurubu (MIS) ve melastan oluşan ortam bileşenlerinin
yüzey yanıt yönteminden (YYY) merkezi birleşik dizayn ile optimize edilmesi
çalışılmıştır. Optimizasyon, dört farklı senaryo üzerinden yapılmıştır. Bunlar,
sadece biyopesitisitin ve proteazın optimizasyonu (Senaryo 1 ve 2), proteazın
yan ürün olduğu biyopesitist optimizasyonu (Senaryo 3) ve biyopesitisitin yan
ürün olduğu proteaz optimizasyonu (Senaryo 4). Eşzamanlı optimizasyonlar,
yüksek miktarlarda ürünlerin elde edilmesinde başarılı sonuçlar vermemiştir.
Ancak, üretim hedefi yan ürünlü üretim şeklinde olursa, en uygun üretim
koşullarına ulaşılabilir. Aynı zamanda, endüstriyel bakış açısıyla incelenecek
olursa, Senaryo 1, büyük ölçekli üretim sistemi açısından uygulanabilir tek
üretim yoludur.

Keywords

Bacillus sphaericus, Biyopesitist, Proteaz, Yanıt yüzey yöntemi (YYY), Melas, Mısır ıslatma şurubu (MIS)

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