Prediction of Bioactive Properties of Walnut Green Husks Using FT-IR Spectroscopy

Year 2018, Volume: 16 Issue: 1, 20 - 26, 23.04.2018

Pınar Kadiroğlu , Halil Ekici

https://doi.org/10.24323/akademik-gida.415643

Cited By: 4

Abstract

Walnut is a widely consumed, nutritive and healthy
crop in the world. China, the US, and Iran are the leading countries of walnut production,
and Turkey is among the main producers ranking fourth with 180.000 tons of
production rate. Walnut green husk is a valuable agricultural by-product that
is commonly used in traditional medicine for alleviating pain and treating skin
diseases related to its antioxidant properties and health benefits. This study
aimed to find the effect of solvent type on extraction of bioactive compounds and
to predict the extraction effects in rapid and reliable method such as Fourier
transform infrared (FT-IR) spectroscopy. Solvent efficiency was evaluated using
different polarity solvents such as water, methanol, ethanol, acetone and
aqueous solutions of methanol, ethanol and acetone for the extraction of walnut
green husks. Analysis of variance results revealed that there was a significant
difference among the total phenolic contents and antioxidant activities of
different extracts. Antimicrobial activity analysis demonstrated that water
extracts showed higher activity against Staphylococcus
aureus. According to the results of partial least squares (PLS) regression
analysis to predict the total phenol content and antioxidant capacity of
extracts, R² values of calibration and cross validation models were
higher than 97% with root mean square error of calibration (RMSEC) and root
mean square error of cross validation (RMSECV) values ranging from 3.03 to
5.35. It can be concluded that PLS models had high predictive capability.

Keywords

Green walnut husks, Extraction, Infrared spectroscopy, Chemometrics

Yeşil Ceviz Kabuklarının Biyoaktif Özelliklerinin FT-IR Spektroskopi Yöntemiyle Tahmin Edilmesi

Abstract

Ceviz (Juglans
regia L.) sağlık ve beslenme açısından çok değerli yağlı tohumlardan
biridir. Dünya ceviz üretiminde ilk sıralarda yer alan ülkelerden Çin, İran ve
ABD’den sonra 180.000 tonluk üretimiyle Türkiye gelmektedir. Ceviz zengin
besleyici öğelere sahip olmakla birlikte sağlık üzerine faydalı özellikleri
bakımından da önemi artarak anlaşılan bir üründür. Cevizin iç kısmının önemi
bilinmekle birlikte ceviz kabuğu genellikle atılmaktadır. Bu çalışmanın amacı, farklı
çözgenler kullanılarak kurutulmuş yeşil ceviz kabuklarından ekstraktlar elde
edilmesi ve bu ekstraktların biyoaktif, antimikrobiyal özelliklerinin ve antioksidan
kapasitesinin belirlenerek Fourier dönüşümlü kızılötesi (FT-IR)  spektroskopisi ile tahmin edilmesidir. Farklı
ekstraktların toplam fenolik madde ve antioksidan aktiviteleri arasında
istatistiksel açıdan önemli bir farklılık bulunmuştur (P<0.05). Antimikrobiyal
analiz sonuçlarına göre ceviz kabuklarının sulu ekstraktları Staphylococcus aureus bakterisi üzerine
etkinliği daha fazla bulunmuştur. FT-IR spektral verinin kısmi en küçük kareler
(PLS) regresyon analizi sonucunda elde edilen tahmini ve ölçülen toplam fenolik
madde ve antioksidan aktivite değerlerinin açıklama (belirtme) katsayısı
>%97, kalibrasyon ve çapraz geçerlilik hatalarının ortalama karekökü
değerleri 3.03 ile 5.35 aralığında bulunmuştur. Elde edilen bulgular PLS
analizi ile oluşturulan modellerin iyi tahmin etme kapasitesine sahip olduğunu
göstermektedir.

Keywords

Yeşil ceviz kabuğu, Ekstraksiyon, İnfrared spektroskopi, Kemometri

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