Screening of Lipid Production Capacities of Bacillus sp. Strains Isolated from Soil and Lipid Staining with Different Staining Techniques

Year 2023, Volume: 23 Issue: 5, 1094 - 1102, 30.10.2023

Elif Demirkan , İrem Yıldırım

https://doi.org/10.35414/akufemubid.1265060

Abstract

Microbial lipids have been attracting more and more attention in recent years as promising raw
materials for the production of biodiesel and value-added compounds. In the current economic and
environmental situation, finding new renewable sources of lipids will be crucial. Recent research has
necessitated the search for new efficient microbial producers with lipid production efficiency. For this
purpose,50 Bacillus sp. strains previously isolated from the soil were screened for their lipid production
capacity. As a result of the production using a single-cell oil production medium, only two Bacillus spp.
strains showed growth. These bacteria were named as Bacillus sp. E40 and Bacillus sp. E226. Bacteria
were then characterized in terms of their biomass, lipid yields, and lipid contents. The biomass of
Bacillus sp. E40 and E226 bacterial isolates, were 0,28 and 0,22 g.L-1, respectively. The highest lipid
content was reached by E226 with 54.5%. Bacillus sp. E40 lipid content was determined as 46.4%. In
addition, both intracellular lipid staining and colony staining demonstrated the presence of lipids. With
this study, it was reported for the first time that local Bacillus sp. strains isolated from soil have lipid
producing capacity.

Keywords

Bacillus, Lipid, Staining, Sudan Black B

Topraktan İzole Edilen Bacillus sp. Suşlarının Lipid Üretim Kapasitelerinin Taranması ve Farklı Boyama Teknikleri ile Lipid Boyama

Abstract

Mikrobiyal lipitler, biyodizel ve katma değerli bileşiklerin üretimi için umut verici hammaddeler olarak
son yıllarda giderek daha fazla dikkat çekmektedir. Mevcut ekonomik ve çevresel durumda, yeni
yenilenebilir lipit kaynakları bulmak çok önemli olacaktır. Son araştırmalar, lipit üretim verimliliğine
sahip yeni verimli mikrobiyal üreticilerin araştırılmasını gerekli kılmıştır. Bu amaçla daha önce topraktan
izole edilen 50 Bacillus sp. suşu lipid üretme kapasiteleri açısından taranmıştır. Tek hücreli yağ üretimi
ortamında yapılan üretim sonucunda bu bakterilerden sadece iki Bacillus sp. suşları üreme göstermiştir.
Bu bakterilerden sadece 2 Bacillus sp. suşu tek hücreli yağ üretimi ortamında üremiştir. Bu bakteriler
Bacillus sp.E40 ve Bacillus sp. E226 olarak adlandırıldı. Bakteriler daha sonra biyokütleleri, lipid verimleri
ve lipid içerikleri açısından karakterize edildi. Bacillus sp. E40 ve E226 bakteri izolatlarının biyokütlesi
sırasıyla, 0,28 ve 0,22 g.L-1 idi. En yüksek lipid içeriğine %54.5 ile E226'da ulaşılmıştır. Bacillus sp. E40
lipid içeriği %46.4 olarak belirlendi. Ek olarak, lipid varlığı hem hücre içi lipid boyama hem de koloni
boyama ile gösterilmiştir Bu çalışma ile topraktan izole edilen yerel Bacillus sp. suşlarının lipit üretme
kapasitesine sahip olduğu ilk kez rapor edilmiştir.

Keywords

Bacillus, Lipid, Boyama, ,Sudan Siyahı B

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