Comparative Phytochemical Contents and Antioxidant Capacities of Three Lepidium Species of the Brassicaceae Family

Year 2024, Volume: 24 Issue: 2, 248 - 254, 29.04.2024

Saliha Aydın , Hakan Terzi , Mustafa Yıldız , Mustafa Kargıoğlu , Ahmet Serteser , Emre Pehlivan

https://doi.org/10.35414/akufemubid.1357106

Abstract

Brassicaceae) species have been studied, information about the chemical compositions and antioxidant activities of some Lepidium species is still insufficient. In the current study, it was aimed to comparatively evaluate the total phenolic and total flavonoid contents and antioxidant capacities of methanol extracts obtained from different tissues of L. draba (LD) and L. perfoliatum (LP) and L. cartilagineum (LC), whose phytochemical contents have not been studied. The highest total phenolic content was determined in flower extracts (153.9 g GAE/mg extract in LC, 143.1 g GAE/mg extract in LD and 118.7 g GAE/mg extract in LP). Total flavonoid contents were highest in leaf extracts (39.3 g QE/mg extract in LP, 34.6 g QE/mg extract in LD and 33.1 g QE/mg extract in LC). The antioxidant capacities of the extracts were analyzed with different antioxidant tests. Similar to the total phenolic content, total antioxidant capacity and free radical scavenging activity were found to be highest in flower tissues. Leaf and flower extracts of LC and LP species showed the highest copper and iron reducing effects. The results indicate that the LC species, as well as the LD and LP species, can be considered a potential medicinal plant with pharmacological properties.

Keywords

Antioxidant, Lepidium cartilagineum, L. draba, L. perfoliatum, Phenolics

Brassicaceae Familyasına ait Üç Lepidium Türünün Karşılaştırmalı Fitokimyasal İçerikleri ve Antioksidan Kapasiteleri

Abstract

Bazı Lepidium (Brassicaceae) türlerinin biyolojik aktiviteleri çalışılmış olmasına rağmen, bazı Lepidium türlerinin kimyasal bileşimleri ve antioksidan aktiviteleri hakkındaki bilgiler halen yetersizdir. Mevcut çalışmada L. draba (LD) ve L. perfoliatum (LP) ile fitokimyasal içeriği çalışılmamış olan L. cartilagineum (LC)’un farklı dokularından elde edilen metanol ekstraktlarında toplam fenolik ve toplam flavonoid içerikleri ile antioksidan kapasitelerinin karşılaştırılmalı olarak değerlendirilmesi amaçlanmıştır. En yüksek toplam fenolik içeriği çiçek ekstraktlarında (LC'de 153.9 g GAE/mg ekstrakt, LD'de 143.1 g GAE/mg ekstrakt ve LP'de 118.7 g GAE/mg ekstrakt) belirlenmiştir. Toplam flavonoid içerikleri ise en yüksek yaprak ekstraktlarında (LP'de 39.3 g QE/mg ekstrakt, LD'de 34.6 g QE/mg ekstrakt ve LC'de 33.1 g QE/mg ekstrakt) saptanmıştır. Ekstraktların antioksidan kapasiteleri farklı antioksidan testleriyle analiz edilmiştir. Çiçek dokularında toplam fenolik içeriğe benzer olarak toplam antioksidan kapasite ve serbest radikal süpürücü aktivite en yüksek bulunmuştur. LC ve LP türlerinin yaprak ve çiçek ekstraktları en yüksek bakır ve demir indirgeyici etki göstermiştir. Sonuçlar, LD ve LP türlerinin yanı sıra LC türünün de farmakolojik özelliklere sahip potansiyel tıbbi bir bitki olarak kabul edilebileceğini göstermektedir.

Keywords

Antioksidan, Lepidium cartilagineum, L. draba, L. perfoliatum, Fenolikler

References

• Ali, Q., Khalil, R., Nadeem, M., Azhar, M., Hafeez, M. and Malik, A., 2020. Antibacterial, antioxidant activities and association among plant growth related traits of Lepidium draba. Biological and Clinical Sciences Research Journal, 11, 1−6.
• Almeida, M.L.B., Freitas, W.E.S., Morais, P.L.D., Sarmento, J.D.A. and Alves, R.E., 2016. Bioactive compounds and antioxidant potential fruit of Ximenia americana L. Food Chemistry, 192, 1078−1082.
• Apak, R., Güçlü, K., Demirata, B., Özyürek, M., Çelik, S. E., Bektaşoğlu, B. and Özyurt, D., 2007. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules, 12, 1496–1547.
• Badarinath, A., Rao, K.M., Chetty, C.M.S., Ramkanth, S., Rajan, T. and Gnanaprakash, K., 2010. A review on in-vitro antioxidant methods: comparisons, correlations and considerations. International Journal of PharmTech Research, 2, 1276–1285.
• Bona, M., 2012. Lepidium taxa in Turkey. Bocconea, 24, 221–225.
• Deng, H. and van Verkel, G.J., 1998. Electrospray mass spectrometry and UV/visible spectrophotometry studies of aluminum (III)-flavonoid complex. Journal Mass Spectrometry, 33, 1080–1087.
• Dias, M.C., Pinto, D.C.G.A. and Silva, A.M.S., 2021. Plant flavonoids: Chemical characteristics and biological activity. Molecules, 26, 5377. Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P. and Vidal, N., 2006. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97, 654–660.
• Eruygur, N., Ayaz, F., Bağcı, Y., Güler, E. and Çağıl, E.M., 2022. Phenolic composition, in-vitro antioxidant and enzyme inhibition activities of Cardaria draba different parts. European Journal of Science and Technology, 35, 424−431.
• Espín, J.C., Soler-Rivas, C. and Wichers, H.J., 2000. Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical. Journal of Agricultural and Food Chemistry, 48(3), 648–656.
• Halkier, B.A. and Gershenzon, J., 2006. Biology and biochemistry of glucosinolates. Annual Reviews in Plant Biology, 57, 303–333.
• Hou, W.C., Lin, R.D., Cheng, K.T., Hung, Y.T., Cho, C.H., Chen, C.H., Hwang, S.Y. and Lee, M.H, 2003. Free radical-scavenging activity of taiwanese native plants. Phytomedicine, 10, 170−175.
• Huang, D., Ou, B. and Prior, R., 2005. The chemistry behind antioxidant capacity assays. Journal of Agriculture and Food Chemistry, 53, 1841–1856.
• Llorent-Martínez, E., Ortega-Barrales, P., Zengin, G., Mocan, A., Simirgiotis, M., Ceylan, R., Uysal, S. and Aktumsek, A., 2017. Evaluation of antioxidant potential, enzyme inhibition activity and phenolic profile of Lathyrus cicera and Lathyrus digitatus: potential sources of bioactive compounds for the food industry. Food and Chemical Toxicology, 107, 609–619.
• Mahomoodally, M.F., Zengin, G., Aumeeruddy, Z.M., Sezgin, M. and Aktümsek, A., 2018. Phytochemical profile and antioxidant properties of two Brassicaceae species: Cardaria draba subsp. draba and Descurainia sophia. Biocatalysis and Agricultural Biotechnology, 16, 453–458.
• Marques, S.S., Magalhães, L.M., Tóth, I.V. and Segundo, M.A., 2014. Insights on antioxidant assays for biological samples based on the reduction of copper complexes—The importance of analytical conditions. International Journal of Molecular Science, 15, 11387–11402.
• Mocan, A., Babotă, M., Pop, A., Fizeșan, I., Diuzheva, A., Locatelli, M., Carradori, S., Campestre, C., Menghini, L., Sisea, C.R., Sokovic, M., Zengin, G., Păltinean, R., Bădărău, S., Vodnar, D.C. and Crișan, G., 2020. Chemical constituents and biologic activities of sage species: A comparison between Salvia officinalis L., S. glutinosa L. and S. transsylvanica (Schur ex Griseb. & Schenk) Schur. Antioxidants, 9(6), 480.
• Moharram, H. and Youssef, M., 2014. Methods for determining the antioxidant activity: a review. Alexandria Journal of Food Science and Technology, 11, 31–42.
• Prieto, P., Pineda, M. and Aguilar, M., 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry, 269, 337–341.
• RiceEvans, C.A. and Miller, N.J., 1996. Antioxidant activities of flavonoids as bioactive components of food. Biochemical Society Transactions, 24, 790–795.
• Schmotzer, A., 2021. New locality of Lepidium cartilagineum in the Hortobágy Region (East Hungary). Acta Biologica Plantarum Agriensis, 9(1), 16–31.
• Sharifi-Rad, J., Hoseini-Alfatemi, S.M., Sharifi-Rad, M., da Silva, J.A.T., Rokni, M. and Sharifi-Rad, M., 2015. Evaluation of biological activity and phenolic compounds of Cardaria draba (L.) extracts. Journal of Biology and Today's World, 4, 180−189.
• Singh, R. and Kumari, N., 2015. Comparative determination of phytochemicals and antioxidant activity from leaf and fruit of Sapindus mukorrossi Gaertn–A valuable medicinal tree. Industrial Crops and Products, 73, 1−8. Singleton, V.L. and Rossi, J.A., 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144–158.
• Sousa, E.O., Miranda, C.M., Nobre, C.B., Boligon, A.A., Athayde, M.L. and Costa J.G., 2015. Phytochemical analysis and antioxidant activities of Lantana camara and Lantana montevidensis extracts. Industrial Crops and Products, 70, 7−15.
• Tuberoso, C.I.G., Rosa, A., Bifulco, E., Melis, M.P., Atzeri, A., Pirisi, F.M. and Dessi, M.A., 2010. Chemical composition and antioxidant activities of Myrtus communis L. berries extracts. Food Chemistry, 123, 1242–1251.
• Wang, Y., Bai, L., Li, H., Yang, W. and Li, M., 2021. Protective effects of Lepidium draba L. leaves extract on testis histopathology, oxidative stress ındicators, serum reproductive hormones and ınflammatory signalling in oxymetholone-treated rat. Andrologia, 53(11), e14239.
• Yousefi, A., Elmarhoum, S., Khodabakhshaghdam, S., Ako, K. and Hosseinzadeh, G., 2022. Study on the impact of temperature, salts, sugars and pH on dilute solution properties of Lepidium perfoliatum seed gum. Food Science and Nutrition, 10, 3955–3968.