The Effect of Acidic Stress on Hematological, Immunological and Biochemical Parameters in Triploid and Diploid Rainbow Trout (Oncorhynchus mykiss)

Year 2017, Volume: 32 Issue: 2, 17 - 24, 29.12.2017

SEVDAN Yılmaz , Ekrem Şanver Çelik , Osman Nezih Kenanoğlu Sebahattin Ergün

https://doi.org/10.28955/alinterizbd.320262

Cited By: 1

Abstract

Early maturity in rainbow trout (Oncorhynchus mykiss) is an economic
loss for fish production industry since flesh quality and appearance get
deformed as metabolic energy is directed from somatic growth into reproduction.
Triploid fish production dramatically reduces this situation, even if it does
not fully wipe it out. However, the resistance of triploid fish against
different stress factors resulted from aquaculture conditions is contentious
compared to diploids. Acidic stress results not only from acid rains, acidic
polluters and acidic waste water, but it might also result from increasing
stock density under aquaculture circumstances. It is already known that acidic
water has negative effects on fish. The present study investigated the effect
of acidic stress on haematological, immunological and serum biochemical
variables of the diploid and triploid rainbow trout, Oncorhynchus mykiss.
Acidic stress was achieved by exposing the sampled fish to acidic water (pH
5.2) for 4 days. Results showed that the red blood cell count, hematocrit,
hemoglobin concentration, mean corpuscular volume, mean corpuscular Hb, and
mean corpuscular Hb concentration, glucose, total protein, albumin, globulin,
triglyceride and cholesterol were not significantly affected by acidic stress
in diploid and triploid fish. However, an acidic stress caused an increase in
the NBT, lysozyme and myeloperoxidase activity of diploid fish. This study
results indicate that the some non-specific immune functions of triploid rainbow
trout did not affect by acidic water.

Keywords

Triploid, Rainbow Trout, Non-Specific Immune Functions, Hematology, Biochemistry

Asidik Stresin Triploid ve Diploid Gökkuşağı Alabalığı(Oncorhynchus mykiss)'nın Hematolojik, İmmunolojik ve Biyokimyasal Kan Parametreleri Üzerine Etkileri

Abstract

Gökkuşağı alabalıklarında erken olgunluğa ulaşma
metabolik enerjinin somatik büyümeden üremeye yönlendirilmesi sonucu et
kalitesinin ve görünümünün bozulması nedeniyle balık üretim endüstrisi için
ekonomik bir kayıptır. Triploid balık üretimi bu durumu tamamen ortadan
kaldırmasa da büyük ölçüde azaltmaktadır. Ancak triploid balıkların diploidlere
oranla yetiştiricilik şartlarından kaynaklı çeşitli stres faktörlerine karşı
dayanıklılıkları tartışma konusudur. Asidik strese; asit yağmurları, asidik
kirleticiler ve asidik pis suların yanı sıra yetiştiricilik şartlarında artan
stok yoğunluğu da neden olabilmektedir. Asidik suların balıklar üzerinde
olumsuz etkileri olduğu bilinmektedir. Bu çalışmada asidik strese maruz
bırakılan diploid ve triploid gökkuşağı alabalıklarının, Oncorhynchus mykiss
hematolojik, immunolojik ve serum biyokimyasal parametrelerindeki değişimler
incelenmiştir. Asidik stres için balıklar asidik suya (pH 5,2) 4 gün maruz
bırakılmıştır. Elde edilen sonuçlar değerlendirildiğinde kırmızı kan hücre
sayısı, hematokrit, hemoglobin, ortalama eritrosit hacmi, ortalama eritrosit
hemoglobini, ortalama eritrosit hemoglobin konsantrasyonu, glikoz, total
protein, albumin, globulin, trigliserit ve kolesterol  diploid ve triploid balıklarda asidik stresten
etkilenmemiştir. Fakat, asidik stres diploid balıklarda NBT, lizozim ve
myeloperoksidaz aktivitelerinde artışa neden olmuştur. Proje sonucunda bazı
spesifik olmayan immun parametrelerinin triploid balıklarda asidik sudan
etkilenmediği tespit edilmiştir.

Keywords

Triploid, diploid, Rainbow trout, spesifik olmayan immun parametreleri, hematoloji, biyokimya

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