INVESTIGATION OF SOME HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF RATS WERE GIVEN VACCINIUM MYRTILLUS (BLUEBERRY) EXTRACT

Year 2018, Volume: 2 Issue: 2, 65 - 72, 02.05.2018

Dide Kılıçalp Kılınç , Seda Nur Aksu Serdal Öğüt

Abstract

Aim: In this study, the differences in hematological and biochemical parameters
were investigated in rats given Vaccinium myrtillus (blueberry)
extract.

Method: Biochemical
parameters albumin (Alb), alkaline phosphatase (ALP), alanine aminotransferase
(ALT), amylase (AMY), blood urea nitrogen (BUN), calcium (Ca), creatinine
(CRE), potassium (K), sodium (Na), phosphorus (Phos), total bilirubin (TBIL),
total protein (TP), fasting glucose (FG), total cholesterol (TC), and
hematologic parameters such as WBC, LYM, MON, NEU, LY, MO, NE, RBC, HGB, HCT
MCV, MCH, MCHC, RDW, PLT, PCT, MPV, PDW levels were measured. In the study,
rats were used as animal material. The rats were separated into two groups,
each of which included 10 rats. Ad libitum fed rats were subjected to the
control group. In the group of blueberry extract for a period of 8 weeks of the
experiment the rate of 20 mg/kg were mixed with drinking water.

Results: Completion
of the process of the experiment, after obtaining blood samples from the above
parameters, creatinine: 0.48 from 0:41 to fell in value. MPV: 6.48 from 6.30 a,
PDW: 33.40 from the 32.48 level have fallen. But no significant changes were
observed outside of these parameters.

Conclusion: As a result, there is a negative impact on blood parameters of
blueberry on the contrary due to the significant decrease in the values of MPV
and PDW, which is a risk factor for hardening of the arteries as a result of
oxidative damage fragmented platelets sticking to the walls of arteries and
reduce the risk of atherosclerosis be considered.

Keywords

Biochemical markers, hematological tests, rat

VACCİNİUM MYRTİLLUS (BLUEBERRY) EKSTRAKTI VERİLEN RATLARDA BAZI HEMATOLOJİK VE BİYOKİMYASAL PARAMETRELERİN ARAŞTIRILMASI

Abstract

Amaç: Bu çalışmada, Vaccinium myritillus (yaban mersini) ekstraktı ile beslenen ratların hematolojik ve biyokimyasal parametrelerinde meydana gelen değişiklikler araştırıldı.

Yöntem: Araştırmada hayvan materyali olarak rat kullanıldı. Ratlar, her grupta 10 adet olacak şekilde 2 ayrı gruba ayrıldı. Kontrol grubundaki ratlar ad libitum beslenmeye tabi tutuldu. Deneme gurubuna ise 8 hafta süreyle yaban mersini ekstraktı 20 mg/kg oranında içme sularına karıştırılarak verildi. Biyokimyasal parametrelerden albumin (Alb), alkalen fosfataz (ALP), alanin aminotransferaz (ALT), amilaz (AMY), kan üre nitrojen (BUN), kalsiyum (Ca⁺⁺), kreatinin (CRE), potasyum (K⁺), sodyum (Na⁺), fosfor (Phos), total bilurubin (TBIL), total protein (TP), açlık kan şekeri (FG), total kolesterol (TK); hematolojik parametreler olan WBC,  LYM, MON, NEU, LY, MO, NE, RBC, HGB, HCT, MCV, MCH, MCHC, RDW PLT, PCT, MPV, PDW değerlerine bakıldı.

Bulgular: Deney süreci tamamlanıp, kan örnekleri alındıktan sonra yukarıdaki parametreler arasından; Kreatinin: 0.48’den 0.41’e düştü. MPV: 6.48’den 6.30’a, PDWc: 33.40’ dan 32.48 düzeyine düştü. Bu parametrelerin dışında kalan parametrelerde anlamlı bir değişiklik gözlenmedi.

Sonuç: Yaban mersininin kan parametreleri üzerinde olumsuz bir etkisinin olmadığı, aksine MPV ve PDW değerlerindeki anlamlı düşüş nedeniyle damar sertliği için bir risk faktörü olan oksidatif zarar sonucu parçalanmış trombositlerin atardamar çeperlerine yapışmasını ve arteroskloroz riskini de azaltabileceği  düşünülebilir.

Keywords

Biyokimyasal belirteçler, kan testleri, rat

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