Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana

Year 2015, Volume: 25 Issue: 1, 17 - 30, 30.06.2015

Meltem Bayraktar , Bärbel Rock Okuyucu

Abstract

 

Hem
sağlık hem de gıda sektörü için önemli bir bitki olan Stevia rebaudiana Bertoni, kalorisi olmayan tatlandırıcı bileşikler
içermektedir ve stevia-kaynaklı sentetik olmayan bu tatlandırıcılar için
talepte sürekli bir artış söz konusudur. Mevcut çalışmanın amacı, S. rebaudiana’nın in vitro ticari üretimi için yüksek steviosid ve rebaudiosid A
içeriğine sahip klonların belirlenmesidir. Bu amaçla, Stevia tohumları in vitro
koşullarda kültüre alınmış ve yüksek çoğaltım oranı ve yüksek steviosid ve
rebaudiosid A içeriği gösteren klon hatları seçilmiştir. Elde edilen iki klon
hattı ticari bir üretici tarafından Stevia’nın
ticari üretimi için adapte edilmiştir. 

 

Keywords

Stevia rebaudiana, in vitro seed germination, in vitro commercial production, HPLC, stevioside, rebaudioside A

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