Kolorektal Kanserde Tiyol–Disülfit Düzeyleri

Year 2019, Volume: 24 Issue: 2, 137 - 141, 13.06.2019

Ayşe Özdemir , Utku Dönem Dilli Dalyan Özdemir Salim Neşelioğlu , Özcan Erel

https://doi.org/10.21673/anadoluklin.469201

Abstract

Amaç: Bu çalışmada kolorektal kanserli hastalarda natif tiyol, disülfit
ve total tiyol konsantrasyonları ile CEA ve CA-19-9 serum biyobelirteçlerini değerlendirmek
ve sonuçları sağlıklı bireylerle karşılaştırmak amaçlanmıştır.

Gereç ve Yöntemler: Çalışmaya 23 kolorektal kanserli hasta ve 23 sağlıklı
birey olmak üzere toplam 46 kişi dahil edildi. Tiyol/disülfit homeostaz
testleri (natif tiyol [-SH], disülfit [-S-S] ve total tiyol [TT]) yeni bir
otomatize metotla gerçekleştirildi. İskemi modifiye albümin (IMA), albümin,
karsinoembriyojenik antijen (CEA), karbonhidrat antijen 19-9 (CA-19-9), TT, -SH
ve -S-S seviyeleri ile disülfit/natif tiyol ve disülfit/total tiyol oranları
gruplar arasında karşılaştırıldı.

Bulgular: Kolorektal kanserli grupta IMA, CEA ve CA-19-9’da kontrol
grubuna kıyasla istatistiksel ola­rak anlamlı farklılık bulundu (p<0,05).
Kontrol grubunda tiyol–disülfit parametreleri ve tümör biyobelir­teçleri arasında
bir ilişki saptanmadı (p>0,05). Kolorektal kanserli hastalarda tiyol–disülfit
parametre­leri ile IMA, albümin, CEA ve CA-19-9 düzeyleri arasında bir ilişki
saptanmadı (p>0,05).

Tartışma ve Sonuç: Çalışmamız noninvaziv bir biyobelirteç olarak natif
tiyol (-SH), disülfit (-S-S) ve total tiyol (TT) konsantrasyonları ile
kolorektal kanser arasında bir ilişki olmadığını göstermektedir.

Keywords

biyokimyasal belirteç, tiyol disülfit, kolorektal neoplazmlar

The Level of Thiol-Disulfide on Colorectal Cancer

Abstract

Aim: This study aimed to evaluate total thiol (TT), disulfide (-S-S),
and native thiol (-SH) concentra­tions as serum biomarkers in patients with
colorectal cancer (CRC).

Materials and Methods: A total of 46 participants (23 patients with
colorectal cancer and 23 healthy individuals) were included. Thiol/disulfide
homeostasis tests (total thiol [TT], native thiol [-SH], and di­sulfide [-S-S])
were performed by a novel automated method. Ischemia modified albumin (IMA),
albu­min, carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA-19-9),
TT, -SH, and -S-S levels as well as disulfide/native thiol and disulfide/total
thiol ratios were compared between the groups.

Results: In the colorectal cancer group, statistically significant difference
was found in IMA, CEA, and CA-19-9, compared to the control group (p<0.05).
There was no relationship between the thiol–disul­fide parameters and tumor
markers in the control group (p>0.05). There was no relationship between the
thiol–disulfide parameters and IMA, albumin, CEA, and CA-19-9 levels in the
colorectal cancer group (p>0.05).

Discussion and Conclusion: Our study shows that the serum concentrations
of native -SH, -S-S, and TT do not link to colorectal cancer as a noninvasive
biomarker.

Keywords

biochemical marker, colorectal neoplasms, disulfide; thiol

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