Histomorphometric Investigation of Changes In Adult Rat Testis After Experimental Vasectomy At Different Periods

Year 2020, Volume: 25 Issue: 2, 130 - 137, 13.06.2020

Alev Cumbul , Varol Şahintürk , Ünal Uslu

https://doi.org/10.21673/anadoluklin.666778

Abstract

Aim: In previous studies, the causes of damage in seminiferous tubules were unclear; if germ cells and supporting cells were involved in the damage and what was the pattern of damage in terms of severity, extent and time course. The aim of this study is to qualitatively and quantitatively analyze the structural changes in seminiferous tubular epithelia thus explain the extent of structural damage of spermatogenic serial in rats.

Material and Methods: In this study, 8 weeks old, 48 male Wistar Albino rats were used. Vasectomy group underwent unilateral testes vasectomy, control group was subjected to abdominal incision. By using cardiac perfusion, left testes of all rats were removed after 1, 12 and 24 weeks (each group n=8). Specimens were immersed in Bouin's solution. Following routine histological procedures, 2 testes of each group stained with Masson trichrome and orcein for qualitative evaluation, 6 testes sectioned randomly (1/8 ratio) and stained with PAS+Hematoxylin. Morphometric data were obtained using stereological methods.

Results: Mean values of numerical density of spermatogonium, Sertoli and myoid cells in 1-12 week vasectomy groups were significantly higher than control. In contrast, amount of spermatids in vasectomy group was observed significantly lower. In vasectomy 24 week group, all cells in seminiferous tubules decreased significantly. In extracellular matrix, an increase was observed in the amount of collagen fibers, and was positively correlated with duration. In vasectomy 1 week group, giant cell formation was observed in lumen.

Conclusion: Two mechanisms may take part. Firstly, compensation mechanism can play role, due to paracrine and intercellular interactions in basal compartment. Secondly, seminal granulom of epididymis may increase the pressure of tubules, thus disrupts the spermatid-sertoli relationship. This may cause sloughing of spermatids in adluminal compartments.

Keywords

Histomorphometry, vasectomy, testis, rat

Deneysel vazektominin farklı süreler sonrasında erişkin sıçan testisinde oluşturduğu değişikliklerin histomorfometrik olarak incelenmesi.

Abstract

Amaç: Yapılan hayvan deneylerinde vazektomi sonrası spermatogenetik seride gözlenen ciddi hasarın oluşum mekanizması tam olarak aydınlatılamamıştır. Bu çalışmanın amacı erişkin sıçanların seminifer tübüldeki spermatogenetik serinin yapısal hasarını nitel ve nicel değişkenler yardımıyla mikroskopik olarak açıklamaya çalışmaktır.

Gereç ve Yöntem: Çalışmada 8 haftalık 48 adet erkek Wistar Albino sıçan kullanıldı. Deney gruplarına tek taraflı vazektomi uygulandı, kontrol grubundaki hayvanlara abdominal kesi yapılıp kapatıldı (her grup için n=8). Deney ve kontrol gruplarındaki sıçanların sol testisleri vazektomiden 1, 12 ve 24 hafta sonrasında perfüzyonla alınarak Bouin’s fiksatifine konuldu. Rutin histolojik takip işleminden sonra her gruptan ikişer adet testis trikrom masson ve orseinle boyanarak ışık mikroskopunda nicel olarak değerlendirildi. Altışar adet testis histomorfometrik inceleme için 1/8 oranında seri kesilerek PAS+Hematoksilenle boyandı. Stereolojik inceleme için Cavalieri ve optik parçalama+optik disektör sondaları kullanıldı.

Bulgular: 1 ve 12 haftalık vazektomi gruplarının spermatagonyum, Sertoli ve myoid hücrelerinin mm3 deki sayılarının kontrole göre arttığı (p<0,001), spermatid sayılarının ise azaldığı (p<0,001) saptandı. 24 haftalık vazektomi grubunda ise seminifer tübülü oluşturan hücrelerin azaldığı görüldü (p<0,001). Nicel değerlendirmede vazektomi sonrası süreye paralel olarak ara maddedeki kollajen lif miktarında artış ve 1 haftalık deney grubunda seminifer tübül lümeninde dev hücre oluşumu gözlemlenmiştir.

Sonuç: Bu bulgulara göre vazektomi sonrasında 12. haftaya kadar seminifer tübül adlüminal ve bazal kompartımanlarında iki farklı mekanizmanın çalıştığı görülmektedir. Bazal kompartımanda gerek parakrin gerekse hücreler arası bağlantılara bağlı bir telafi mekanizması çalışmaktadır. Epididimiste gelişen seminal granülomunun, seminifer tübül iç basıncını artırarak adlüminal kompartımanda spermatid sertoli ilişkisini bozduğu, bunun spermatidlerin olgunlaşamadan hızla dejenere olmasına neden olduğu düşünülmektedir.

Keywords

Vazektomi, Testis, Sıçan, Histomorfometri

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