Deneysel overyan iskemi/reperfüzyon modelinde iloprostun uzak organ olarak karaciğer dokusu üzerindeki koruyucu etkisi

Year 2022, Volume: 27 Issue: 1, 71 - 76, 29.01.2022

Mehmet Duran , Nezir Yılmaz , Ebru Annaç , Mevlüt Doğukan

https://doi.org/10.21673/anadoluklin.1030797

Abstract

Amaç: Bu deneysel çalışmada iloprostun karaciğer dokusu üzerindeki koruyucu etkisini bir iskemi/reperfüzyon (İ/R) sıçan modelinde araştırmak amaçlanmıştır.

Yöntem: Toplam 32 adet dişi Sprague–Dawley sıçan, 4 eşit gruba ayrıldı. Grup 1’deki sıçanlarda overyan dokulara herhangi bir cerrahi işlem uygulanmadı. Sırasıyla Grup 2, 3 ve 4’teki overyan dokulara ise 3 saatlik torsiyon, 3 saatlik torsiyon + 3 saatlik detorsiyon, ve 3 saatlik torsiyon + 3 saatlik detorsiyon + 60 dakikalık iloprost infüzyonu uygulandı. Dokulardan 7 μm kalınlıkta kesitler alındı, hematoksilen–eozin, Masson trikrom ve toluidin mavisi ile boyandı. Kesitler üzerinde ışık mikroskobik inceleme yapıldı.

Bulgular: Grup 2, 3 ve 4’te Grup 1’e kıyasla sinüzoidal dilatasyon ve vasküler konjesyonda istatistiksel olarak anlamlı artış tespit edildi (p<0,05). Grup 4’te diğer gruplara kıyasla daha fazla sinüzoidal dilatasyon ve vasküler konjesyon görüldü. Yine Grup 2, 3 ve 4’te Grup 1’e kıyasla daha fazla enflamasyon, fibrozis, hemoraji ve daha yüksek mast hücre yoğunluğu tespit edildi. Ancak fark istatistiksel olarak anlamlı değildi (p>0,05). Histopatolojik skorlamada Grup 2 ve 4’te benzer, Grup 3’te ise daha yüksek skorlar gözlendi.

Sonuç: İloprostun İ/R hasarına karşı karaciğer dokusu üzerinde koruyucu etkileri olduğu sonucuna varılmıştır.

Keywords

iloprost, iskemi/reperfüzyon, karaciğer hasarı

Supporting Institution

yok

Project Number

yok

Thanks

Doç.Dr. Öznur Uludağ

The protective effect of iloprost on liver tissue as a remote organ in an experimental model of ovarian ischemia/reperfusion

Abstract

Aim: In this experimental study, we aimed to investigate the protective effect of iloprost on liver tissue in a rat model of ischemia/reperfusion (I/R).

Methods: A total of 32 female Sprague–Dawley rats were divided into 4 equal groups. The ovarian tissues of the rats in Group 1 did not undergo any surgical procedure. The ovarian tissues of Group 2, 3, and 4 underwent 3-hour of torsion, 3-hour detorsion + 3-hour torsion, and 3-hour torsion + 3-hour detorsion + 60-minute iloprost infusion, respectively. Sections of 7 μm thickness were taken, stained with hematoxylin–eosin, Masson trichrome, and toluidine blue, and examined with light microscopy.

Results: Compared to Group 1, a statistically significant increase in sinusoidal dilatation and vascular congestion was found in Group 2, 3, and 4 (p<0.05). Compared to the other groups, more sinusoidal dilatation and vascular congestion were observed in Group 4. Similarly, Group 2, 3, and 4 showed more inflammation, fibrosis and hemorrhage and higher mast cell density compared to Group 1, although the difference was not statistically significant (p>0.05). In the histopathological scoring, Group 2 and 4 had similar scores while Group 3 scored higher.

Conclusion: It was concluded that iloprost had protective effects on liver tissue against I/R injury.

Keywords

iloprost, ischemia/reperfusion, liver injury

Project Number

yok

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