Determination of Biochemical Content and Antioxidant Activity of Calliergonella cuspidata (Hedw.) Loeske

Year 2024, Volume: 10 Issue: 1, 25 - 33, 01.06.2024

Dilay Turu , Selime Deniz Bozkurt , Cenker Yaman , Gizem Gül , Atakan Benek , Kerem Canlı

https://doi.org/10.26672/anatolianbryology.1434173

Abstract

Since ancient times, humans have been utilizing various plants for medicinal purposes, a practice that has persisted from early civilizations to the present day. Plants serve as rich sources of biomolecules, although many of their contents remain unidentified. Bryophytes are considered important reservoirs for new natural products, with mosses being less explored compared to liverworts despite their broader species diversity. This study contains the content analysis of ethanol, methanol, and n-hexane extracts of Calliergonella cuspidata to predict and compare their biochemical compound profiles. The ethanol extract revealed the presence of 3-Formyl-N-methyl-9-[phenylethynyl]dibenzo2,3-a:5,6-a'-thiazine and Eicosane, while Beta-Elemene and Neophytadiene were identified in the methanol extract, and predominantly alkanes were found in the n-hexane extract. Subsequently, antioxidant activity was determined using the DPPH method with the ethanol extract, yielding an EC50 value of 1.0237 mg/ml.

Keywords

Bryophytes, Moss, Calliergonella cuspidata, GC/MS, Antioxidant, Türkiye

Calliergonella cuspidata (Hedw.) Loeske'nin Biyokimyasal İçeriğinin ve Antioksidan Aktivitesinin Belirlenmesi

Abstract

İnsanların çok eski çağlardan beri çeşitli amaçlarla yararlandığı bitkilerin tıbbi amaçlı kullanımı ilk uygarlıklardan günümüze kadar devam etmiştir. Bitkiler oldukça zengin biyomolekül kaynakları oluştururlar. Ancak birçoğunun içerik tanımları henüz yapılmamıştır. Briyofitlerin yeni doğal ürünler için önemli bir rezervuar olduğu düşünülmektedir. Karayosunları ise daha geniş bir tür çeşitliliğine sahip olmasına rağmen, ciğerotlarına göre daha az araştırılmıştır. Bu çalışma, biyokimyasal bileşik profillerini tahmin etmek ve karşılaştırmak için Calliergonella cuspidata'nın etanol, metanol ve n-heksan ekstraktlarının içerik analizini içermektedir. Etanol ekstraktında 3-Formil-N-metil-9-[feniletinil]dibenzo[2,3-a:5,6-a'](1,4)-tiazin ve Eikosan, metanol ekstraktında Beta-Elemen ve Neofitadien, N-hekzan ekstraktında ise ağırlıklı olarak alkanlara rastlanmıştır. Daha sonra etanol ekstraktı ile DPPH yöntemi kullanılarak antioksidan aktivite belirlenmiştir. EC50 değeri 1,0237 mg/ml olarak tespit edilmiştir.

Keywords

Briyofitler, Karayosunları, Calliergonella cuspidata, GC/MS, Antioksidan, Türkiye

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