Melatonin Extraction and Determination by UHPLC-FD from Different Plants

Year 2024, Volume: 3 Issue: 2, 220 - 228, 18.10.2024

Yusuf Güllüoğlu Enes Yararlı , Ahmet Murat Ahmet Turan Tay Yılmaz Uğur , Zeynep Maraş Selim Erdoğan

Abstract

Many plants have become a piece of the traditional food as human diet and evaluated in folk medicine to treat diseases. They are very rich in terms of nutraceuticals. Melatonin functions as a free radical scavenger and controls the regulation of the sleep–wake cycle in mammals. In this study, we analyzed melatonin content in the herba parts of Stachys lavandulifolia, Tribulus terrestris, and Helichrysum arenarium, and unripe fruits of Prunus armeniaca L., Prunus cerasifera, and Prunus amygdalus L. A solvent mixture of methanol:ultrapure water:HCl (70:29.9:01 v/v/v) was used for the extraction of melatonin from plant samples. The determination of melatonin in the extracts was carried out by high pressure liquid chromatography with fluorescence detector (UHPLC-FD). Melatonin concentration was 0.0231 mg/kg for herba of Helichrysum arenarium and 0.0018 mg/kg for Prunus armeniaca L. Melatonin was not detected in other plant samples.

Keywords

Helianthus annuus, Prunus armeniaca L., Melatonin

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