E VİTAMİNİ VE ÇÖREK OTU YAĞI İÇEREN POLİ-3-HİDROKSİBUTİRAT-KO-3- HİDROKSİHEKSAONAT-JELATİN MİKROFİBER TEKSTİL LİFLERİ: ANTİBAKTERİYEL ETKİNLİK VE YARA İYİLEŞMESİNE KATKI

Year 2024, Volume: 38 Issue: 2, 37 - 52, 30.08.2024

Emine Kutlu , Fatih Mehmet Emen , Naciye Erkan , Cansu Olguner , Ece Kutlu , Kumar Sudesh

https://doi.org/10.54962/ankemderg.1475030

Abstract

Mikrofiber tekstil lifleri ile yara bakımında avantaj sağlayabilen ürünler geliştirilebilmektedir. Bu çalışmada poli-3-hidroksibutirat-ko-3-hidroksiheksaonat-jelatin içeren mikrofiber tekstil lifleri (PJ) ile E vitamini ve çörek otu yağı içeren ve biyolojik olarak parçalanabilen poli-3-hidroksibutirat-ko-3-hidroksiheksaonat-jelatin mikrofiber tekstil lifleri (PJ-ÇE) elektrospinning yöntemi kullanılarak hazırlandı. Liflerin yapısal karakterizasyonları Fourier Dönüşümlü Kızılötesi Spektroskopisi (FT-IR) ile gerçekleştirildi. Mikrofiber tekstil liflerinin termal davranışları Termogravimetri (TG)/Diferansiyel Termal Analiz (DTA)/Diferansiyel Termogravimetri (DTG) kombine sistemiyle, yüzey morfolojileri ise Taramalı Elektron Mikroskop (SEM) tekniği ile incelendi. PJ ve PJ-ÇE liflerinin antibakteriyel etkinlikleri, tekstillerin antibakteriyel aktivite test yöntemiyle (JIS L 1902: 2002) Escherichia coli ATCC 35150 ve Staphylococcus aureus ATCC 25923 suşlarına karşı araştırıldı. PJ ve PJ-ÇE liflerinin yara iyileşmesine in vitro etkisi L929 fibroblast hücreleri üzerinde incelendi. Elektrospinning tekniğiyle dayanıklı PJ ve PJ-ÇE lifleri elde edilebildiği yapısal testlerle gösterilmiştir. Antibakteriyel çalışma sonuçlarına göre PJ liflerinin E. coli üzerinde yüksek, S. aureus üzerinde ise düşük antibakteriyel aktiviteye sahip olduğu belirlenmiştir. PJ-ÇE liflerinin antibakteriyel etkinliği ise, PJ liflerine göre E. coli üzerinde daha düşük, S. aureus üzerinde daha yüksek bulunmuştur. PJ-ÇE liflerine maruz kalan L929 fibroblast hücrelerinde hasarın onarımında artış gözlenmiştir. PJ-ÇE liflerinin yeni kompozit yara örtülerinin geliştirilmesinde kullanım açısından umut vaat ettiği düşünülmüştür.

Keywords

elektrospinnig, mikrofiber örtüler, poli(3-hidroksibutirat-ko-3-hidroksiheksanoat, jelatin, antibakteriyel aktivite, yara iyileşmesi.

Poly 3 Hydroxybutyrate Co 3 Hydroxyhexaonate Gelatin Microfiber Textile Fibers Containing Vitamin E and Black Cumin Oil: Antibacterial Activity and Contribution to Wound Healing

Abstract

Products that can provide advantages in wound care can be developed with microfiber textile fibers. In this study, microfiber textile fibers containing poly-3-hydroxybutyrate-co-3-hydroxyhexaonate-gelatin (PJ) and biodegradable poly-3-hydroxybutyrate-co-3-hydroxyhexaonate-gelatin microfiber textile fibers containing vitamin E and black cumin oil (PJ-ÇE) were prepared using electrospinning method. Structural characterization of the fibers was carried out by Fourier Transform Infrared Spectroscopy (FT-IR). The thermal behavior of the microfiber textile fibers was investigated by Thermogravimetry (TG)/Differential Thermal Analysis (DTA)/Differential Thermogravimetry (DTG) combined system and their surface morphology was investigated by Scanning Electron Microscopy (SEM) technique. The antibacterial activities of PJ and PJ-ÇE fibers were investigated against Escherichia coli ATCC 35150 and Staphylococcus aureus ATCC 25923 strains by the textile antibacterial activity test method (JIS L 1902: 2002). The in vitro effect of PJ and PJ-ÇE fibers on wound healing was investigated on L929 fibroblast cells. It was shown by structural tests that durable PJ and PJ-ÇE fibers can be obtained by electrospinning technique. According to the results of the antibacterial study, PJ fibers were found to have high antibacterial activity on E. coli and low antibacterial activity on S. aureus. The antibacterial activity of PJ-ÇE fibers was found to be lower on E. coli and higher on S. aureus compared to PJ fibers. An increase in damage repair was observed in L929 fibroblast cells exposed to PJ-ÇE fibers. PJ-ÇE fibers were thought to be promising for use in the development of new composite wound dressings.

Keywords

antibacterial activity, Electrospinning, gelatin, microfiber dressings, poly(3 hydroxybutyrate co 3 hydroxyhexanoate, wound healing

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