Soya Bitkisindeki Glutatyon Redüktaz Aktivitesi ve mRNA Seviyesinin Kuraklık Stresinde Salisilik Asit ile Değişimleri

Year 2017, Volume: 5 Issue: 2, 48 - 52, 31.05.2017

ESEN Taşğın , HAYRUNNİSA Nadaroğlu , AHMET Adıgüzel , M.ÖZKAN Baltacı , ZEYNEP Sönmez

https://doi.org/10.21541/apjes.290973

Cited By: 1

Abstract

Bu çalışma, kuraklık stresi ve
salisilik asitin (SA) soya bitkisindeki etkilerini değerlendirmek için
gerçekleştirilmiştir. Bu amaçla, su stresine maruz bırakılan soya bitkisindeki
SA, reaktif oksijen türleri (ROS) ve glutatyon redüktaz (GR)
enzimi arasındaki ilişki incelenmiştir. Soya fasulyesi (Glycine max L. cv.) bitkileri serada kum tepsilere ekilerek
büyütülmüştür. İkinci yaprak tamamen çıktığında bitkilerin yarısı bir hafta süreyle
kuraklığa maruz bırakılmıştır. Bir haftanın sonunda hem kontrol hemde kuraklık
stres grupları SA (200 μmol/L) ile muamele edilmiş ve iki gün sonra tüm
gruplardan kesimler alınmıştır. Kontrol, kontrol+SA, kuraklık, kuraklık+SA
muameleli yapraklarda glutatyon redüktaz aktivitesi ve mRNA seviyeleri ölçülmüştür.
Bu çalışmada, 200 μmol/L SA muamelesi kontrol
şartlarında GR aktivitesini önemli ölçüde artırmıştır. Kuraklık muameleli
yapraklarda, kontroller ile karşılaştırıldığında, GR enzim aktivitesinin
arttığı gözlenmiştir. Ancak su stresi altında SA muamelesi ile GR enzim
aktivitesi önemli ölçüde azalmıştır. GR enzimini kodlayan genlerin
transkripsiyon seviyeleri real-time PCR (Polimeraz zincirleme tepkimesi)
kullanılarak ölçülmüştür. SA muamelesi kuraklığa maruz kalan soya yapraklarının
GR-RNA seviyelerini hızlı bir şekilde azaltmıştır.

Keywords

Glutatyon redüktaz, kuraklık, salisilik asit, soya

Glutathione Reductase Activity in Soybean Plants and Changes in mRNA Levels with Salicylic Acid in Drought Stress

Abstract

This study was carried to evaluate
the effect of drought stress and salicylic acid (SA) treatments in soybean
plants. Soybean (Glycinemax L. cv.) plants were grown to sown in trays of sand
in greenhouse. When the second leaf was fully expanded, half of the plants were
exposed to drought stress for one week. At the end of one week, half of the
plants in the both control and drought stress groups were treated with SA (200
μmol/L) and two days later was taken cuttings from the whole. The activities of
glutathione reductase (GR) and levels of mRNA have been measured in control, control-SA treatment, drought
treament and drought-SA treatment leaves. In this study, 200 μmol/L SA
treatment significantly has been increased GR activity in control conditions.
Drought treated leaves have been observed elevated in the activities of the GR
enzyme, compared to controls. But, under medium water deficit, GR activity
significantly reducing with SA treatment. The transcript levels of the genes
encoding GR enzyme have been measured using quantitative real-time PCR
(Polymerase Chain Reaction). SA treatment has decreased rapidly GR -RNA levels
of soybean leaves exposed to drought stress.

Keywords

Glutathione reductase, drought, salicylic acid, soybean

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