DNA-binding and spectroscopic studies of cobalt (II) compound containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile

Year 2017, Volume: 5 Issue: 3, 1 - 9, 01.10.2017

Ali Arslantas , Mehmet Salih Ağırtaş

https://doi.org/10.21541/apjes.329065

Cited By: 1

Abstract

In this study, binding activities of the
previously synthesized cobalt
(II) compound (PcCo) with calf thymus-DNA containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1,
3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile were investigated to
determine binding activities by using UV-Vis absorption, fluorescence titration
methods. Absorption titration spectra of PcCo in DMF give peaks at about 670 nm
for Q-band absorption and about 340 nm for B-band absorption in the absence of
calf thymus-DNA. Emission intensities of PcCo
in presence of calf thymus-DNA, PcCo gives
emission at about 545 nm. Thermal
denaturation profile study was systematically studied by UV/Vis spectroscopic
technique and melting point temperaturewas found around 7.87 oC
for Co (II) phthalocyanine compound.  The
reduction oxidation behavior of PcCo with
calf thymus-DNA was investigated using cyclic voltammetry (CV). Cathodic peak potential and anodic peak
potential for PcCo were recorded to be 0.84, -0.36 V for EPc and 0.88 V, 0.15
V, and -0.69 V EPa. The cyclic
voltammetric results show one half- reversible reduction oxidation wave. The
shift in Epc and Epa potentials indicates that PcCo shows strong interaction with calf thymus-DNA. Further to
understand binding activities of PcCo
with calf thymus-DNA was studied by using electrophoresis technique. Electrophoresis
findings showed that there is decrease in the intensity of CT-DNA bands. All the findings show that PcCo interacts strongly with DNA molecule via an intercaltion binding.

Keywords

Phthalocyanines, DNA-binding, UV/Vis spectroscopy, gel electrophoresis, cyclic voltammetry.

2, 10, 16, 24-Tetrakis 4- (2-fenoksi-l, 3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren kobalt (II) bileşiğinin DNA bağlanmasının spektroskopik yöntemler ile araştırılması

Abstract

2,10, 16, 24-tetrakis 4-
(2-fenoksi-1,3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren önceden
sentezlenmiş kobalt (II) bileşiğinin (PcCo)
CT-DNA ile bağlanma özellikleri UV/Vis absorpsiyon, floresan titrasyonu
yöntemleri kullanılarak bağlanma özelliklerini belirlemek için araştırıldı.
Termal denatürasyon profili çalışması UV-Vis spektroskopik tekniği ile
sistematik olarak incelenmiş ve Co (II) ftalosiyanin bileşiği için erime
noktası sıcaklık değeri 7.87 oC civarında bulunmuştur. CT-DNA
yokluğunda ve varlığında PcCo'in
redüksiyon oksidasyon davranışı, dönüşümlü voltametri (CV) ile incelendi.
Dönüşümlü (cyclic) voltametri sonuçlar, bir yarı-tersinir redaksiyon oksidasyon
dalgasını göstermektedir. Epc ve Epa potansiyelindeki değişim, PcCo'in CT-DNA'yla güçlü etkileşim gösterdiğini
önermektedir. PcCo'in CT-DNA ile
olan etkileşimi ayrıca jel elektroforez yöntemi kullanılarak araştırılmıştır.
Jel elektroforezi sonuçları, CT-DNA bantlarının yoğunluğunda azalma olduğunu
gösterdi. Tüm bulgular, PcCo ile
CT-DNA molekülü arasındaki etkileşimin interkalatif bağlanma türü olduğunu
göstermektedir.

Keywords

DNA, DNA-bağlanma, Ftalosiyaninler, Jel Elektroforez, UV/Vis spektroskopi

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