KRİYOJELLERİN AFİNİTE UYGULAMASI

Year 2019, Volume: 2 Issue: 2, 9 - 15, 31.12.2019

Gözde Baydemir Peşint , Burcu Eren Okan Zenger

Abstract

Cryogels
are macroporous cross-linked polymeric structures. Affinity chromatography is a
type of chromatography based on some adsorption purposes, which is either
naturally occurring or artificially formed in biological systems. The wide
pores and superior choice properties of cryogels have enabled these materials
to be used in different affinity applications. The types of affinity
chromatography were quite common when some of the biological biomacromolecules
were separated. Advantages of cryogels have been utilized in applications for
separation, purification and molecular recognition. In this review, the
production of cryogels and affinity chromatography types have been summerized.

Keywords

Affinity chromatography

References

• [1] Reichelt, S. (2015). Affinity chromatography: Methods and protocols. New York: Humana Press.[2] Kahveci, M.U., Beyazkilic, Z., Yagci, Y. (2010). Polyacrylamide cryogels by photoinitiated free radical polymerization. J. Polym. Sci: Part A: Polym. Chem., 48, 4989–4994.[3] Hajizadeh, S., Kirsebom, H., Leistner, A., Mattiasson, B. (2012). Composite cryogel with immobilized concanavalin A for affinity chromatography of glycoproteins. J. Sep. Sci., 35, 2978–2985.[4] Kirsebom, H., Topgaard, D., Galaev, I.Y., Mattiasson, B. (2010). Modulating the porosity of cryogels by influencing the nonfrozen liquid phase through the addition of inert solutes. Langmuir, 26, 16129–16133.[5] Garcia, F.A.P., Pires, K.M.V. (1993). in: J.F. Kennedy, J.M.S. Cabral (Eds.), Chromatography, Wiley, London, p. 415.[6] Akgöl, S., Denizli, A. (2004). Novel metal-chelate affinity sorbents for reversible use in catalase adsorption. J. Mol. Catal., 28, 7–14.[7] Karatas, M., Akgöl, S., Yavuz, H., Say, R., Denizli, A. (2007). Immunoglobulin G depletion from human serum with metal chelated beads under magnetic field. Int. J. Biol. Macromol., 40, 254–260.[8] Kumar, A., Bansal, V., Andersson, J., Roychoudhury, P.K., Mattiasson, B. (2006). Supermacroporous cryogel matrix for integrated protein isolation- Immobilized metal affinity chromatographic purification of urokinase from cell culture broth of a human kidney cell line. Journal of Chromatography A, 1103(1), 35-42.[9] Cimen, D., Denizli, A. (2012). Immobilized metal affinity monolithic cryogels for cytochrome c purification. Colloids Surf. B: Biointerfaces, 93, 29–35.[10] Andac¸ C.A., Andac, M., Denizli, A. (2007). Predicting the binding properties of cibacron blue F3GA in affinity separation systems. Int. J. Biol. Macromol., 41, 430–438.[11] Dogan, A., Özkara, S., Sari, M.M., Uzun, L., Denizli, A. (2012). Evaluation of human interferon adsorption performance of Cibacron Blue F3GA attached cryogels and interferon purification by using FPLC system. J. Chromatogr. B, 893(894), 69–76.[12] Deraz, S., Plieva, F.M., Galaev, I.Yu., Karlsson, E.N., Mattiasson, B. (2007). Capture of bacteriocins directly from non-clarified fermentation broth using macroporous monolithic cryogels with phenyl ligands. Enzyme Microbial. Technol., 40, 786–793.[13] Yilmaz, F., Bereli, N., Yavuz, H., Denizli, A. (2009). Supermacroporous hydrophobic affinity cryogels for protein chromatography. Biochem. Eng. J., 43, 272–279.[14] Li, H., Liu, Z. (2012). Recent advances in monolithic column-based boronate-affinity hromatography. Trends Anal. Chem., 37, 148–161.[15] Saylan, Y., Bereli, N., Uzun, L., Denizli, A. (2014). Monolithic boronate affinity columns for IgG. Sep. Sci. Technol., 49, 1555–1565.[16] Bereli, N., Ertürk, G., Tümer, M.A., Say, R., Denizli, A. (2013). Oriented immobilized anti-hIgG via Fc fragment-imprinted PHEMA cryogel for IgG purification. Biomed. Chromatogr., 27, 599–607.[17] Andac, M., Galaev, I.Y., Denizli, A. (2013). Molecularly imprinted poly(hydroxyethyl methacrylate) based cryogel for albumin depletion from human serum. Colloids Surf. B: Biointerfaces, 109, 259–265.[18] Fatoni, A., Numnuam, A., Kanatharana, P., Limbut, W., Thavarungkul, P. (2014). A novel molecularly imprinted chitosan–acrylamide, graphene, ferrocene composite cryogel biosensor used to detect microalbumin. Analyst, 139, 6160–6167.[19] Rabieizadeh, M., Kashefimofrad, S.M., Naeimpoor, F. (2014). Monolithic molecularly imprinted cryogel for lysozyme recognition. J. Sep. Sci., 37, 2983–2990.[20] Ertürk, G., Bereli, N., Ramteke, P.W., Denizli, A. (2014). Molecularly imprinted supermacroporous cryogels for myoglobin recognition. Appl. Biochem. Biotechnol., 173, 1250–1262.