Research Article
BibTex RIS Cite

Investigation of Blood Natural Killer Cell Activity in Normozoospermic Fertile, Oligozoospermic, Severe-Oligozoospermic, Oligoastenozoospermic, Azoospermic and Idiopathic Individuals

Year 2023, Volume: 3 Issue: 2, 218 - 221, 11.08.2023
https://doi.org/10.58252/artukluder.1260015

Abstract

Introduction: Infertility is a health problem that negatively affects couples and society in many ways and is becoming increasingly common. It is estimated that 30-40% of these problems are caused by men. It was aimed to determine whether natural killer (Natural Killer: NK) cell activity changes in male individuals with different degrees of infertility problems and in individuals with normal sperm count and concentration (normozoospermia).
Methods: NK cell activities were measured from blood samples taken from individuals who were determined to be oligozoospermia, severe-oligozoospermia, oligoastenoozospermia, azoospermia and idiopathic as a result of semen analysis with from individuals diagnosed normozoospermia (n:120).
Results: NK cell activity in blood was observed as the lowest in normozoospermia (544.46 pg/ml), but the highest value measured in severe oligozoospermia group (1005.90 pg/ml). On the other hand, NK cell activity was measured 797.60 ± 428.55 pg/ml, 905.34 ± 430.60 pg/ml, 757.66 ± 541.16 pg/ml and 639.44 ± 385.50 pg/ml in oligozoospermia, oligoastenozoospermia, azoospermia and idiopathic groups respectively. While the difference in NK activity between the severe oligozospermia group and the other groups was significant (p<0.05), the differences between the other groups were not significant (p >0.05).
Conclusion: It was determined that NK cell activity was higher in individuals with severe oligozoospermia than normozoospermia, oligozoospermia, oligoastenozoospermia, azoospermia and idiopathic individuals.

References

  • 1Vayena E, Rowe P, Griffin P. Current practices and controversies in assisted reproduction: report of a meeting on medical, ethical and social aspects of assisted reproduction, held at WHO Headquarters in Geneva, Switzerland. World Health Organization, 2002.
  • Practice Committee of American Society for Reproductive Medicine. Definitions of infertility and recurrent pregnancy loss. Fertil Steril 2008;89(6):1603.
  • Garenne ML, Frish RE. Natural fertility. Infertility Reproductive Med. Clin. North America. 1994;5:159-282.
  • Gomel V, Urman B, Yarali H. Investigation of the infertile couple. Vaksel S, Beksac S, editors. Reproductive endocrinology and infertility medical network. Ankara:1993;143-55.
  • Shirshov VN. Current state of the male infertility problem: The review of European Association of Urology clinical guidelines. J.Clin. Pract. 2016;7:39–50. doi:10.17816/clinpract7139-50
  • Epanchintseva EA, Selyatitskaya VG, Mitrofanov IM, Pinkhasov BB, Sviridova MA. Antisperm antibodies under infertility in males, connection with abdominal obesity. Adv. Curr. Nat. Sci. 2015;4:24–27.
  • Dorofeev SD, Efremov EA, Simakov VV. Management of idiopathic pathospermia. Effective pharmacotherapy. Urol. Nefrol. 2015;2:24–30.
  • Xu F, Ye L, Hu Y, Cai C, Wang Z, Fan L, et al. A novel protein biochip screening serum anti-sperm antibody expression and natural pregnancy rate in a follow-up study in Chinese infertility. Biosci. Rep. 2020;40(2). doi:10.1042/BSR20191769
  • Silva CA, Cocuzza M, Carvalho JF, Bonfá E. Diagnosis and classification of autoimmune orchitis. Autoimmun. Rev. 2014;13:431–434. doi: 10.1016/j.autrev.2014.01.024
  • Wald M. Male infertility: Causes and cures. Sex. Reprod. Menopause. 2005;3:83–87. doi: 10.1016/j.sram.2005.09.006
  • Schmidt, AA, Zamyatnin SA, Gonchar IS, Korovin AE. Risk factors of the development of male infertility. Clin. Pathophysiol. 2019;25:56–60.
  • Avadieva NE. The use of DNA semen fragmentation in andrological practice. Vestn. Urol. 2019;7:7–11.
  • Likhonosov, NP, Ayub AK, Babenko A, Borovets S. The role of inhibin B in the regulation of spermatogenesis and its clinical significance in male infertility. Urol. Vedom. 2019;9:39–45. doi: 10.17816/uroved9139-45
  • Apolikhin OI, Moskaleva NG, Komarova VA. Contemporary demographic situation and problems of improving the reproductive health of Russian population. Exp. Clin. Urol. 2015;4:4–14.
  • Lutov YV, Selyatitskaya VG, Epanchintseva EA, Ryabinchenko TI. The relationship between the andrological status of adolescents with anthropometric and hormonal parameters. Hum. Physiol. 2014;40:124–131. doi: 10.1134/S0362119714040100
  • Jiang Y, Cui D, Du Y, Lu J, Yang L, Li J. et al. Association of anti-sperm antibodies with chronic prostatitis: A systematic review and meta-analysis. J. Reprod. Immunol. 2016;118:85–91. doi: 10.1016/j.jri.2016.09.004
  • Paoli D, Gilio B, Piroli E, Gallo M, Lombardo F, Dondero F. et al. Testicular tumors as a possible cause of antisperm autoimmune response. Fertil. Steril. 2009;91:414–419. doi: 10.1016/j.fertnstert.2007.11.084
  • Joki-Korpela P, Sahrakorpi N, Halttunen M, Surcel HM, Paavonen J, Tiitinen A. The role of Chlamydia trachomatisinfection in male infertility. Fertil Steril. 2009;91:1448– 1450. doi: 10.1016/j.fertnstert.2008.06.051
  • Hulusi BZ, Hakan Y. Antisperm antibodies: Fact or fiction? Immunol. Allergy Clin. N. Am. 2002;22:471–501. doi: 10.1016/S0889-8561(02)00010-3
  • Kurpisz M, Havryluk A, Nakonechnyj A, Chopyak V, Kamieniczna, M. Cryptorchidism and long-term consequences. Reprod. Biol. 2010;10:19–35. doi: 10.1016/S1642-431X(12)60035-7
  • Bozkırlı İ. Erkek infertilitesi. Bozkırlı, İ. (eds), Yeni Üroloji. Ankara: Türk Hava Kurumu Basımevi; 1999.
  • Yang C, Zhou ZH, Zheng DN, Xu XF, Haung J, Lian Y, Qiao J. Sperm origins and concentration do not impact the clinical outcomes in intracytoplasmic sperm injection cycles. Asian J of Androl 2018;20:1-5. doi: 10.4103/aja.aja_27_18
  • Usta A, Erdem E, Karacan M, Cebi Z, Uluğ M, Arvas A, et al. The effect of the sperm source on the outcome of intracytoplasmic sperm injection-embryo cycles in normal responder women. Acta Med. Anatol. 2016;4;2.
  • Mazzilli R, Cimadomo D, Vaiarelli A, Capalbo A, Dovere L, Alviggi E, et al. Effect of the male factor on the clinical outcome of intracytoplasmic sperm injection combined with preimplantation aneuploidy testing: observational longitudinal cohort study of 1,219 consecutive cycles. Fertil Steril. 2017;108(6):961-972. doi: 10.1016/j.fertnstert.2017.08.033
  • Lydyard P, Grossi C. Cells involved in the immune response, In: Roitt IM, Brossotoff J, Male DK. (eds) Immunology. Forth edition. Spain. Mosby Com, 1996;2(2);2-14.
  • Lotzova E. Natural killer cells: İmmonobiology and clinical prospects. Cancer İnvest. 1991;9:173-84. doi: 10.3109/07357909109044228
  • Tekeli A, Hatice Özenci H. Doğal öldürücü hücreler. Flora. 1997;2;138-145.
  • Öztürk ÖG. Doğal öldürücü hücre ımmunglobulin-benzeri reseptör genleri. Arşiv Kaynak Tarama Dergisi 2011;20(3):131-144.
  • Pennarrubia G, Koster F T. Antibacterial activity of human natural killer cells. J Exp Med 1989;169:99-113. doi: 10.1084/jem.169.1.99
  • Duan YG, Gong J, Yeung WS, Haidl G, Allam JP. Natural killer and NKT cells in the male reproductive tract. J. Reprod. Immunol. 2020;103178. doi: 10.1016/j.jri.2020.103178
  • Narayanan P, Ashalatha P. (2021). Prevalence of Abnormal Karyotypes among Males with Non-obstructive Azoospermia and Severe Oligozoospermia: A Retrospective Study. J. Clin. Diagn. Res. 2021;15(2):9-14. doi: 10.7860/JCDR/2021/47876.14568
  • Gong J, Zeng Q, Yu D, Duan YG. T lymphocytes and testicular immunity: a new insight into immune regulation in testes. Int. J. Mol. Sci. 2021;22(1);57. doi: 10.3390/ijms22010057
  • Duan YG, Chen S, Haidl G, Allam JP. Detection of invariant natural killer T cells in ejaculates from infertile patients with chronic inflammation of genital tract. Am. J. Reprod. Immunol. 2017;78(2);e12671. doi: 10.1111/aji.12671

Normozoospermik Fertil Bireyler ile Oligozoospermik, Şiddetli-Oligozoospermik, Oligoastenozoospermik, Azoospermik ve İdiyopatik Bireylerde Natural Killer Hücre Aktivitesinin Araştırılması

Year 2023, Volume: 3 Issue: 2, 218 - 221, 11.08.2023
https://doi.org/10.58252/artukluder.1260015

Abstract

Giriş: İnfertilite çiftleri ve toplumu birçok açıdan olumsuz etkileyen ve gittikçe yaygınlaşan bir sağlık problemidir. Bu problemlerin %30-40’ının erkek kaynaklı olduğu tahmin edilmektedir. Farklı derecelerde infertilite problemi olan erkek bireyler ile normal sperm sayısı ve konsantrasyonuna sahip bireylerde (normozoospermi) doğal öldürücü (Natural Killer: NK) hücre aktivitesinin değişip değişmediğinin belirlenmesi amaçlanmıştır.
Gereç ve Yöntem: Sperma analizleri sonucu oligozoospermi, şiddetli-oligozoospermi, oligoastenoozospermi, azoospermi ve idiopatik olduğu belirlenen bireyler ile normozoospermi teşhisi konan bireylerden (n:120) alınan kan numunelerinden NK hücre aktiviteleri ölçüldü.
Bulgular: Yapılan ölçümler sonucunda normozoospermi grubu en düşük değer (544.46 pg/ml) alırken şiddetli oligozoospermi grubundan en yüksek değer (1005.90 pg/ml) alınmıştır. NK hücre aktivitesi ise oligozoospermi, oligoastenozoospermi, azoospermi ve idiyopatik gruplarda sırasıyla 797.60 ± 428.55 pg/ml, 905.34 ± 430.60 pg/ml, 757.66 ± 541.16 pg/ml ve 639.44 ± 385.50 pg/ml olarak ölçüldü. Şiddetli oligozoospermi grubu ile diğer gruplar arasında NK aktivitesi farkı önemli (p<0.05) bulunurken diğer gruplar arasındaki farklar önemli bulunmamıştır (p >0.05).
Sonuç: İnfertilite derecesi şiddetli oligozoospermi olan bireylerde NK hücre aktivitesinin normozoospermi, oligozoospermi, oligoastenozoospermi, azoospermi ve idiopatik gruplarından daha yüksek olduğu belirlenmiştir.

References

  • 1Vayena E, Rowe P, Griffin P. Current practices and controversies in assisted reproduction: report of a meeting on medical, ethical and social aspects of assisted reproduction, held at WHO Headquarters in Geneva, Switzerland. World Health Organization, 2002.
  • Practice Committee of American Society for Reproductive Medicine. Definitions of infertility and recurrent pregnancy loss. Fertil Steril 2008;89(6):1603.
  • Garenne ML, Frish RE. Natural fertility. Infertility Reproductive Med. Clin. North America. 1994;5:159-282.
  • Gomel V, Urman B, Yarali H. Investigation of the infertile couple. Vaksel S, Beksac S, editors. Reproductive endocrinology and infertility medical network. Ankara:1993;143-55.
  • Shirshov VN. Current state of the male infertility problem: The review of European Association of Urology clinical guidelines. J.Clin. Pract. 2016;7:39–50. doi:10.17816/clinpract7139-50
  • Epanchintseva EA, Selyatitskaya VG, Mitrofanov IM, Pinkhasov BB, Sviridova MA. Antisperm antibodies under infertility in males, connection with abdominal obesity. Adv. Curr. Nat. Sci. 2015;4:24–27.
  • Dorofeev SD, Efremov EA, Simakov VV. Management of idiopathic pathospermia. Effective pharmacotherapy. Urol. Nefrol. 2015;2:24–30.
  • Xu F, Ye L, Hu Y, Cai C, Wang Z, Fan L, et al. A novel protein biochip screening serum anti-sperm antibody expression and natural pregnancy rate in a follow-up study in Chinese infertility. Biosci. Rep. 2020;40(2). doi:10.1042/BSR20191769
  • Silva CA, Cocuzza M, Carvalho JF, Bonfá E. Diagnosis and classification of autoimmune orchitis. Autoimmun. Rev. 2014;13:431–434. doi: 10.1016/j.autrev.2014.01.024
  • Wald M. Male infertility: Causes and cures. Sex. Reprod. Menopause. 2005;3:83–87. doi: 10.1016/j.sram.2005.09.006
  • Schmidt, AA, Zamyatnin SA, Gonchar IS, Korovin AE. Risk factors of the development of male infertility. Clin. Pathophysiol. 2019;25:56–60.
  • Avadieva NE. The use of DNA semen fragmentation in andrological practice. Vestn. Urol. 2019;7:7–11.
  • Likhonosov, NP, Ayub AK, Babenko A, Borovets S. The role of inhibin B in the regulation of spermatogenesis and its clinical significance in male infertility. Urol. Vedom. 2019;9:39–45. doi: 10.17816/uroved9139-45
  • Apolikhin OI, Moskaleva NG, Komarova VA. Contemporary demographic situation and problems of improving the reproductive health of Russian population. Exp. Clin. Urol. 2015;4:4–14.
  • Lutov YV, Selyatitskaya VG, Epanchintseva EA, Ryabinchenko TI. The relationship between the andrological status of adolescents with anthropometric and hormonal parameters. Hum. Physiol. 2014;40:124–131. doi: 10.1134/S0362119714040100
  • Jiang Y, Cui D, Du Y, Lu J, Yang L, Li J. et al. Association of anti-sperm antibodies with chronic prostatitis: A systematic review and meta-analysis. J. Reprod. Immunol. 2016;118:85–91. doi: 10.1016/j.jri.2016.09.004
  • Paoli D, Gilio B, Piroli E, Gallo M, Lombardo F, Dondero F. et al. Testicular tumors as a possible cause of antisperm autoimmune response. Fertil. Steril. 2009;91:414–419. doi: 10.1016/j.fertnstert.2007.11.084
  • Joki-Korpela P, Sahrakorpi N, Halttunen M, Surcel HM, Paavonen J, Tiitinen A. The role of Chlamydia trachomatisinfection in male infertility. Fertil Steril. 2009;91:1448– 1450. doi: 10.1016/j.fertnstert.2008.06.051
  • Hulusi BZ, Hakan Y. Antisperm antibodies: Fact or fiction? Immunol. Allergy Clin. N. Am. 2002;22:471–501. doi: 10.1016/S0889-8561(02)00010-3
  • Kurpisz M, Havryluk A, Nakonechnyj A, Chopyak V, Kamieniczna, M. Cryptorchidism and long-term consequences. Reprod. Biol. 2010;10:19–35. doi: 10.1016/S1642-431X(12)60035-7
  • Bozkırlı İ. Erkek infertilitesi. Bozkırlı, İ. (eds), Yeni Üroloji. Ankara: Türk Hava Kurumu Basımevi; 1999.
  • Yang C, Zhou ZH, Zheng DN, Xu XF, Haung J, Lian Y, Qiao J. Sperm origins and concentration do not impact the clinical outcomes in intracytoplasmic sperm injection cycles. Asian J of Androl 2018;20:1-5. doi: 10.4103/aja.aja_27_18
  • Usta A, Erdem E, Karacan M, Cebi Z, Uluğ M, Arvas A, et al. The effect of the sperm source on the outcome of intracytoplasmic sperm injection-embryo cycles in normal responder women. Acta Med. Anatol. 2016;4;2.
  • Mazzilli R, Cimadomo D, Vaiarelli A, Capalbo A, Dovere L, Alviggi E, et al. Effect of the male factor on the clinical outcome of intracytoplasmic sperm injection combined with preimplantation aneuploidy testing: observational longitudinal cohort study of 1,219 consecutive cycles. Fertil Steril. 2017;108(6):961-972. doi: 10.1016/j.fertnstert.2017.08.033
  • Lydyard P, Grossi C. Cells involved in the immune response, In: Roitt IM, Brossotoff J, Male DK. (eds) Immunology. Forth edition. Spain. Mosby Com, 1996;2(2);2-14.
  • Lotzova E. Natural killer cells: İmmonobiology and clinical prospects. Cancer İnvest. 1991;9:173-84. doi: 10.3109/07357909109044228
  • Tekeli A, Hatice Özenci H. Doğal öldürücü hücreler. Flora. 1997;2;138-145.
  • Öztürk ÖG. Doğal öldürücü hücre ımmunglobulin-benzeri reseptör genleri. Arşiv Kaynak Tarama Dergisi 2011;20(3):131-144.
  • Pennarrubia G, Koster F T. Antibacterial activity of human natural killer cells. J Exp Med 1989;169:99-113. doi: 10.1084/jem.169.1.99
  • Duan YG, Gong J, Yeung WS, Haidl G, Allam JP. Natural killer and NKT cells in the male reproductive tract. J. Reprod. Immunol. 2020;103178. doi: 10.1016/j.jri.2020.103178
  • Narayanan P, Ashalatha P. (2021). Prevalence of Abnormal Karyotypes among Males with Non-obstructive Azoospermia and Severe Oligozoospermia: A Retrospective Study. J. Clin. Diagn. Res. 2021;15(2):9-14. doi: 10.7860/JCDR/2021/47876.14568
  • Gong J, Zeng Q, Yu D, Duan YG. T lymphocytes and testicular immunity: a new insight into immune regulation in testes. Int. J. Mol. Sci. 2021;22(1);57. doi: 10.3390/ijms22010057
  • Duan YG, Chen S, Haidl G, Allam JP. Detection of invariant natural killer T cells in ejaculates from infertile patients with chronic inflammation of genital tract. Am. J. Reprod. Immunol. 2017;78(2);e12671. doi: 10.1111/aji.12671
There are 33 citations in total.

Details

Primary Language Turkish
Subjects Clinical Sciences
Journal Section Research Articles
Authors

M. Ferit Özmen 0000-0002-5531-220X

Muhamet Afşin 0000-0002-7625-3982

Uğur Şeker 0000-0002-1693-6378

Dilek Yavuz 0000-0002-2877-533X

Muhammed Hanifi Bademkıran 0000-0002-9350-582X

Ümüt Cirit 0000-0002-0187-2615

İbrahim Yıldırım 0000-0001-5518-5004

Early Pub Date July 13, 2023
Publication Date August 11, 2023
Submission Date March 6, 2023
Published in Issue Year 2023 Volume: 3 Issue: 2

Cite

APA Özmen, M. F., Afşin, M., Şeker, U., Yavuz, D., et al. (2023). Normozoospermik Fertil Bireyler ile Oligozoospermik, Şiddetli-Oligozoospermik, Oligoastenozoospermik, Azoospermik ve İdiyopatik Bireylerde Natural Killer Hücre Aktivitesinin Araştırılması. Artuklu International Journal of Health Sciences, 3(2), 218-221. https://doi.org/10.58252/artukluder.1260015
AMA Özmen MF, Afşin M, Şeker U, Yavuz D, Bademkıran MH, Cirit Ü, Yıldırım İ. Normozoospermik Fertil Bireyler ile Oligozoospermik, Şiddetli-Oligozoospermik, Oligoastenozoospermik, Azoospermik ve İdiyopatik Bireylerde Natural Killer Hücre Aktivitesinin Araştırılması. Artuklu International Journal of Health Sciences. August 2023;3(2):218-221. doi:10.58252/artukluder.1260015
Chicago Özmen, M. Ferit, Muhamet Afşin, Uğur Şeker, Dilek Yavuz, Muhammed Hanifi Bademkıran, Ümüt Cirit, and İbrahim Yıldırım. “Normozoospermik Fertil Bireyler Ile Oligozoospermik, Şiddetli-Oligozoospermik, Oligoastenozoospermik, Azoospermik Ve İdiyopatik Bireylerde Natural Killer Hücre Aktivitesinin Araştırılması”. Artuklu International Journal of Health Sciences 3, no. 2 (August 2023): 218-21. https://doi.org/10.58252/artukluder.1260015.
EndNote Özmen MF, Afşin M, Şeker U, Yavuz D, Bademkıran MH, Cirit Ü, Yıldırım İ (August 1, 2023) Normozoospermik Fertil Bireyler ile Oligozoospermik, Şiddetli-Oligozoospermik, Oligoastenozoospermik, Azoospermik ve İdiyopatik Bireylerde Natural Killer Hücre Aktivitesinin Araştırılması. Artuklu International Journal of Health Sciences 3 2 218–221.
IEEE M. F. Özmen, M. Afşin, U. Şeker, D. Yavuz, M. H. Bademkıran, Ü. Cirit, and İ. Yıldırım, “Normozoospermik Fertil Bireyler ile Oligozoospermik, Şiddetli-Oligozoospermik, Oligoastenozoospermik, Azoospermik ve İdiyopatik Bireylerde Natural Killer Hücre Aktivitesinin Araştırılması”, Artuklu International Journal of Health Sciences, vol. 3, no. 2, pp. 218–221, 2023, doi: 10.58252/artukluder.1260015.
ISNAD Özmen, M. Ferit et al. “Normozoospermik Fertil Bireyler Ile Oligozoospermik, Şiddetli-Oligozoospermik, Oligoastenozoospermik, Azoospermik Ve İdiyopatik Bireylerde Natural Killer Hücre Aktivitesinin Araştırılması”. Artuklu International Journal of Health Sciences 3/2 (August 2023), 218-221. https://doi.org/10.58252/artukluder.1260015.
JAMA Özmen MF, Afşin M, Şeker U, Yavuz D, Bademkıran MH, Cirit Ü, Yıldırım İ. Normozoospermik Fertil Bireyler ile Oligozoospermik, Şiddetli-Oligozoospermik, Oligoastenozoospermik, Azoospermik ve İdiyopatik Bireylerde Natural Killer Hücre Aktivitesinin Araştırılması. Artuklu International Journal of Health Sciences. 2023;3:218–221.
MLA Özmen, M. Ferit et al. “Normozoospermik Fertil Bireyler Ile Oligozoospermik, Şiddetli-Oligozoospermik, Oligoastenozoospermik, Azoospermik Ve İdiyopatik Bireylerde Natural Killer Hücre Aktivitesinin Araştırılması”. Artuklu International Journal of Health Sciences, vol. 3, no. 2, 2023, pp. 218-21, doi:10.58252/artukluder.1260015.
Vancouver Özmen MF, Afşin M, Şeker U, Yavuz D, Bademkıran MH, Cirit Ü, Yıldırım İ. Normozoospermik Fertil Bireyler ile Oligozoospermik, Şiddetli-Oligozoospermik, Oligoastenozoospermik, Azoospermik ve İdiyopatik Bireylerde Natural Killer Hücre Aktivitesinin Araştırılması. Artuklu International Journal of Health Sciences. 2023;3(2):218-21.

by-nc.svg AIJHS journal and all articles published in AIJHS are licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.