Investigation of the Impact of Can-filling Medium on the DNA Quality of Canned Tuna Sold in Supermarkets

Year 2022, Volume: 37 Issue: 4, 183 - 187, 20.10.2022

Elif Tugce Aksun Tumerkan

https://doi.org/10.26650/ASE202221031790

Abstract

Canned tuna is one of the most commonly consumed food products globally. Due to its high profit-ability and the increasing demand for it, fraudulent canned tuna products have become a serious problem. The traceability of fish species in packaged material and, in the case of highly processed forms, in canned products, has become impossible; therefore, canned tuna is on the list of the top ten food items affected by fraud. These fraudulent actions cause not only unfair trade in the commer-cial market and fishing industry, but also cause health damage (such as allergies and poisoning) to the public. Complex food matrices also affect the extracted DNA quality when the main food products are served with another medium. Brine solutions, different kind of oil, and several types of sauce are used as filling medium in the canned tuna production process. These filling medium can cause con-tamination depending on whether they include oil, salt or other ingredients during DNA extraction from main products. DNA-based protocols have become popular due to their higher reliability rate compared to other protocols. This research investigates the potential impact of can-filling medium on DNA quality, which is a key factor for food traceability research. With this aim, canned tuna from various brands in different can-filling medium such as olive oil, sunflower oil and different kinds of sauces, were obtained from a Turkish supermarket. The quality properties, such as yield and purity, affected the traceability analyses. This study was designed to investigate the potential effect of the filling medium on DNA quality. The results revealed that different kinds of sauce utilization as a can-filling medium cause a reduction in the DNA quality of canned tuna compared to other canned tuna samples that contain olive oil and sunflower oil. The purity of extracted DNA in canned tuna where olive oil was used was found to be relatively higher than other tuna groups with different can-filling medium. Melting curve analyses revealed that sunflower oil causes relatively lower degra-dation than olive oil and different types of sauce used as filling medium. These results could be beneficial for further seafood traceability research, especially in complex matrices.

Keywords

Canned tuna, filling medium, DNA quality, DNA yield, traceability

References

• Abdullah, A. & Rehbein, H. (2015). The differentiation of tuna (family: Scombridae) products through the PCR-based analysis of the cytochrome b gene and parvalbumin introns. Journal of the Science of Food and Agriculture, 96(2), 456-464. doi:10.1002/jsfa.7111 google scholar
• Asche, F. Bellemare, M.F. Roheim, C. Smith, M.D. & Tveteras, S. (2015). Fair enough? Food security and the international trade of seafood. World Development, 67, 151-160. google scholar
• Ballari, R. V. & Martin, A. (2013). Assessment of DNA degradation induced by thermal and UV radiation processing: Implications for quantification of genetically modified organisms. Food chemistry, 141(3), 2130-2136. google scholar
• Barbosa, C. Nogueira, S. Gadanho, M. & Chaves, S. (2016). DNA extraction: finding the most suitable method. Molecular Microbial Diagnostic Methods, 135-154. doi:10.1016/b978-0-12-416999-9.00007-1 google scholar
• Barendse, J. Roel, A. Longo, C. Andriessen, L. Webster, L. M. I. & Ogden, R., (2019). DNA barcoding validates species labelling of certified seafood. Current Biology. 29(6), R198-R199 https://doi.org/10.1016/j. cub.2019.02.014. google scholar
• Boughattas, F. Le Fur, B. & Karoui, R. (2019). Identification and quantification oftuna species in canned tunas with sunflower medium by means of a technique based on front face fluorescence spectroscopy (FFFS). Food Control, 101, 17-23. https://doi. org/10.1016/j.foodcont.2019.02.003 google scholar
• Chapela, M. J. Sotelo, C. G. Perez-Martın, R. I. Pardo, M.Â. Perez-Villareal, B. & Gilardi, P., (2007). Comparison of DNA extraction methods from muscle of canned tuna for species identification. Food Control, 18, 1211e1215. google scholar
• Elsanhoty, R. Ramadan, M.F. & Jany, K.D. (2011). DNA extraction methods for detecting genetically modified foods: a comparative study. Food chemistry, 126, 1883-1889 google scholar
• Europe, O. (2015). Too cheap to be true: Seafood fraud in Brussels. Fact Sheet google scholar
• FAO, (2018). State of World Fisheries and Aquaculture. Food & Agriculture Organization. google scholar
• Fox, M. Mitchell, M. Dean, M. Elliott, C. & Campbell, K. (2018). The seafood supply chain from a fraudulent perspective. Food Security, 10(4), 939-963. google scholar
• Gordoa, A. Carreras, G. Sanz, N. & Vinas, J. (2017). Tuna species substitution in the Spanish commercial chain: A knock-on effect. PloS One., 12(1), e0170809 google scholar
• Julien-David, D. & Marcic, C. (2020). Food, nutrition and health in France. In S. Braun, C. Zübert, D. Argyropoulos, & F. J. C. Hebrard (Eds.), Nutritional & Health Aspect— Traditional & Ethnic Food, Nutritional and Health Aspects of Food in Western Europe (s. 109-131). Academic Press. ISBN 978-0-12-813171 google scholar
• Lucena-Aguilar, G. Sânchez-Lopez, A. M. Barberân-Aceituno, C. Carrillo-Avila, J. A. Lopez-Guerrero, J. A. & Aguilar-Quesada, R. (2016). DNA source selection for downstream applications based on DNA quality indicators analysis. Biopreservation and Biobanking, 14(4), 264-270. google scholar
• Pardo, M. Â. & Jimenez, E. (2020). DNA barcoding revealing seafood mislabelling in food services from Spain. Journal of Food Composition and Analysis, 103521. doi:10.1016/j.jfca.2020.103521 google scholar
• Piskata, Z. Pospisilova, E. & Borilova, G. (2017). Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue. International Journal of Food Properties, 20, p 430443. google scholar
• Reilly, A. (2018). Overview of food fraud in the fisheries sector. FAO Fisheries and Aquaculture Circular, (C1165), I-21. google scholar
• Sotelo, C. G. Velasco, A. Perez-Martin, R. I. Kappel, K. Schröder, U. Verrez-Bagnis, V. ... & Griffiths, A. (2018). Tuna labels matter in Europe: Mislabelling rates in different tuna products. PLOS ONE, 13(5), e0196641. doi:10.1371/journal.pone.0196641 google scholar
• Sajali, N. Wong, S. C. Hanapi, U. K. Abu Bakar Jamaluddin, S. Tasrip, N. A. & Mohd Desa, M. N. (2018). The Challenges of DNA Extraction in Different Assorted Food Matrices: A Review. Journal of Food Science, 83(10), 2409-2414. doi:10.1111/1750-3841.14338 google scholar
• Tamm, E. E. Schiller, L. & Hanner, R. H. (2016). Seafood traceability and consumer choice. In A. M. Naaum, & R. H. Hanner (Eds.), Seafood authenticity and traceability, a DNA based perspective. (1st ed., pp. 27e45). London, UK: Elsevier. Academic Press. ISBN 978-0-12801592-6. google scholar
• Ward, R. D. Zemlak, T. S. Innes, B. H. Last, P. R. & Hebert, P. D. (2005). DNA barcoding Australia’sfish species. Philosophical Transactions of the Royal Society B: Biological Sciences, 360(1462), 1847-1857. google scholar