NİSTATİN VE TİTANYUM DİOKSİT İLAVESİNİN YUMUŞAK ASTAR MATERYALİNDEKİ CANDIDA KOLONİZASYONU ÜZERİNE ETKİSİNİN KARŞILAŞTIRILMASI

Year 2018, Volume: 28 Issue: 1, 48 - 54, 28.03.2018

Funda Bayındır Esra Kul , Ayşe Esin Aktaş Ayşe :baştopçu

https://doi.org/10.17567/ataunidfd.410379

Abstract

Amaç: Tam protez
kullanan hastalarda, yumuşak astar materyalleri, mukozayı irrite eden lezyon
oluşumları ile sonuçlanan, Candida
albicans’ ların adezyonunu
COMPARISON OF THE EFFECT OF NYSTATIN AND TITANIUM DIOXIDE ADDITION ON CANDIDA COLONIZATION IN SOFT LINING MATERIAL






ABSTRACT
ve kolonizasyonunu destekleyebilir. Bu
çalışmanın amacı, nistatin ve titanium dioksit [TiO₂]
antifungal ajanlarının, yumuşak astar maddelerindeki
Candida albicans kolonizasyonunu
önlemedeki etkisini değerlendirmektir.

Gereç ve Yöntem: Ufi Gel P
ve Mollosil yumuşak astar materyallerinden 5mm çapında ve 2mm kalınlığında, 5%, 10%, and 15% konsantrasyonda
nistatin ve titanyum dioksit içeren diskler
hazırlandı. Kontrol grubu için ise nistatin ve TiO₂
içermeyen, sadece yumuşak astar maddesinden oluşan diskler hazırlandı. C.
albicans’ lar Sabouraud
Dekstroz Agar (SDA) kaplı besiyerine inoküle edildi. Hazırlanan diskler
besiyerlerine yerleştirildi. 37° C’de inkübe
edildi.

Çalışmanın
ikinci basamağı için yeniden hazırlanan 10% w/v nistatin içeren diksler  zamanla antifungal etkinin değerlendirilmesi
için farklı süreler (1, 7, 14, and 16 gün) boyunca su içinde bekletildiler.
Daha sonra sudan çıkarılıp, C. albicans
inoküle edilmiş SDA plağına yerleştirildiler. Her gruba nistatin veya TiO₂
içermeyen bir kontrol diski yerleştirildi.

Bulgular: Uygulanan varyans analizi sonucu
kontrol disklerinde istatistiksel olarak anlamlı bir C. albicans inhibisyonu yoktu (p
<0.001). İn vitro olarak nistatin’in toz formunun yumuşak astarlara
eklenmesi, C. albicans kolonizasyonunu
önledi. Disklerin suda bekleme süresi, inhibisyon derecesini etkiledi. Fakat,
yumuşak astarlara TiO₂
ilavesi, C. albicans kolonizasyonunu
önlemedi.

Sonuç: Bu
çalışmanın sonucuna göre, in vitro olarak, nistatinin toz formunun yumuşak
astarlara ilave edilmesi, C. albicans kolonizasyonunu önledi fakat aynı miktardaki TiO₂
tozu eklenmesi
etki göstermedi.

Anahtar Kelimeler: Nistatin,
titanyum dioksit, Candida albicans

COMPARISON OF THE EFFECT OF NYSTATIN AND
TITANIUM DIOXIDE ADDITION ON CANDIDA COLONIZATION IN SOFT LINING MATERIAL

ABSTRACT

Aim: Soft liners can support the adhesion and colonization
of Candida albicans, which can then
irritate the mucosa of denture wearers, resulting in the formation of lesions.The
goal of this study was to evaluate the efficacy of fungicidal agents, nystatin
and titanium dioxide [TiO₂], in preventing the colonization by Candida albicans on soft lining
materials.

Material and methods: Disks of Ufi Gel P and Mollosil cold-curing, soft
relining materials with a diameter of 5 mm and thickness of 2 mm were prepared
with nystatin and TiO₂ powders in a concentration of 5%, 10%, and
15% (w/v). For the control group, only discs not containing nystatin and TiO₂
were prepared, which consisted only of soft lining material. C. albicans was inoculated on Sabouraud
dextrose agar (SDA)-coated plates. The disks were placed on these plates and
incubated aerobically at 37° C.

The Ufi Gel P and Mollosil disks, including 10% w/v
nystatin, (re-prepared for the second step of the study) were immersed in water
for separate intervals (1, 7, 14, and 16 days) to determine the fungicidal action
over time. The disks were then removed and placed on SDA plates inoculated with
C. albicans. In each group, one
control disk without nystatin or TiO₂ was included.

Results: A univariate analysis of variance test revealed that the activity of C. albicans was not significantly
inhibited on the control disks (p <
0.001). The addition of nystatin in powder form to the soft liners prevented
the colonization of C. albicans in vitro. The immersion time of the
disks plunged in water appeared to affect the degree of inhibition. However, the addition of TiO₂ did
not prevent colonization by C. albicans
of the soft liners.

Conclusion: This study showed that the addition of nystatin in powder form to soft
liners prevented the colonization of C.
albicans in vitro but that the
addition of the same amount of TiO₂ powder did not.

Keywords: Nystatin,
titanium dioxide, Candida albicans

Keywords

Nistatin, titanyum dioksit

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