The Effect of Delaying the Administration of Second GnRH in the Ovsynch Protocol on Pregnancy Rate in Dairy Cows

Year 2014, Volume: 9 Issue: 3, 187 - 193, 23.12.2014

Birten Emre , Ömer Korkmaz Abuzer Kafar Zonturlu

https://doi.org/10.17094/avbd.46313

Cited By: 1

Abstract

The aim of this study was to evaluate the effect of delaying the second GnRH injection on pregnancy rates in the Ovsynch protocol (OVS) in cows. In the study, a total of 40 primiparous, Holstein-Friesian dairy cows treated with 10 μg GnRH on day zero and 500 μg PGF2α on day 7 and the cows were then randomly divided into two groups. The 2nd GnRH injection was performed at the 48 hours in the OVS 48 (n = 21) and at the 56 hours in the OVS 56 (n = 19) after the PGF2α injection. Artificial insemination (AI) was performed 16 hours after the protocols. Blood samples were taken at day 7 and at the time of insemination to evaluate progesterone (P4). Pregnancy diagnosis was determined at 40-45th day after the AI by transrectal ultrasonography. No statistically significant differences were determined between groups for the mean serum P4 values on day 7 and at the time of insemination (OVS 48: 9.11 ± 7.60 and 0.89 ± 1.31 ng/ml vs. OVS 56: 6.00 ± 5.98 and 1.10 ± 1.79 ng/ml, respectively) (P>0.05). Pregnancy rates were determined as 66.66 % in group OVS 48 and 47.36 % in group OVS 56. However, no significant difference was observed between the groups (P>0.05). In conclusion, the findings suggest that delaying the second GnRH injection for 8 hours (56th hour instead of 48th hour) in conventional Ovsynch protocol had no effect on pregnancy rate in dairy cows

Keywords

Dairy Cow, Gonadotropin-releasing hormone, Ovsynch, Pregnancy rate

Sütçü İneklerde Ovsynch Protokolünde İkinci GnRH Uygulamasının Geciktirilmesinin Gebelik Oranı Üzerine Etkisi

Abstract

Bu çalışmada, ineklerde Ovsynch (OVS) protokolünde ikinci GnRH enjeksiyonunun geciktirilmesinin gebelik oranı üzerine etkisinin araştırılması amaçlandı. Çalışmada, toplam 40 adet primipar, Holstein-Friesian ırkı sağmal ineğe 0. günde 10 μg GnRH ve 7. günde 500 μg PGF2α uygulanarak rastgele iki gruba ayrıldı. Prostaglandin uygulamasını takiben hayvanlara (n=21), 48. saatte (OVS 48), diğer gruptaki hayvanlara (n=19) ise 56. saatte 2. GnRH enjeksiyonu yapıldı (OVS 56). Protokolleri takiben 16. saatte suni tohumlama uygulandı. Serum progesteron (P4) değerlendirilmesi amacıyla, PGF2α’nın uygulandığı 7. günde ve tohumlamalar sırasında kan örnekleri alındı. Gebelik tanısı, suni tohumlamadan sonraki 40-45. günde transrektal ultrasonografi ile tespit edildi. Gruplar arasındaki 7. gün ve tohumlama anı ortalama serum P4 değerlerinin değişimi incelendiğinde, (OVS 48: sırasıyla 11.09 ± 7.60 ve 0.89 ± 1.31 ng/ml olmasına karşın, OVS 56: 6.00 ± 5.98 ve 1.10 ± 1.79 ng/ml) istatistiksel olarak önemli bir farklılık belirlenmedi (P>0.05). Çalışmada, farklı Ovsynch programları sonucunda elde edilen gebelik oranı OVS 48 grubunda % 66.66, OVS 56 grubunda ise % 47.36 olarak belirlendi. Ancak, gruplar arasındaki farklılığın istatistiksel olarak önemli olmadığı gözlendi (P>0.05). Sonuç olarak, sütçü ineklerde klasik Ovsynch protokolünde ikinci GnRH enjeksiyonunun 8 saat geciktirilmesinin (48 yerine 56. saatte) gebelik oranı üzerine herhangi bir etkisinin olmadığı kanısına varıldı

Keywords

Gebelik oranı, Gonadotropin-releasing hormon, Ovsynch, Sütçü İnek

References

• Aydın İ., Aköz M., Dinç DA., 2008. Post partum dönemdeki süt ineklerinde modifiye edilmiş Ovsynch protokolünün ovulatör follikül gelişimi ve gebelik oranı üzerine etkisi. III Veteriner Jinekoloji Kongresi, 23-26 Ekim, Antalya.
• Bahrami A., Mosaferi S., Hamali H. and Ostadi Z., 2012. Comparative study of resynchronization conception rate based on ovsynch 48 and 56 hour in dairy cow. Res. J. of Biol. Sci., 7(4), 175-180.
• Baumann LE., 1988. Monitoring and predicting reproductive performance in dairy herds. Vol I and II. Dissertation Abstracts International, B-Sciences and Engineering, 49(4), 1049.
• Bisinotto RS., Ribeiro ES., Martins LT., Marsola RS., Greco LF., Favoreto MG., Risco CA., Thatcher WW., Santos JEP., 2010. Effect of interval between induction of ovulation and artificial insemination (AI) and supplemental progesterone for resynchronization on fertility of dairy cows subjected to a 5-d timed AI program. J. Dairy Sci., 93: 5798-5808.
• Brusveen DJ., Cunha AP., Silva CD., Cunha PM., Sterry RA., Silva EP., Guenther JN., Wiltbank MC., 2008. Altering the time of the second gonadotropin-releasing hormone injection and artificial insemination (AI) during Ovsynch affects pregnancies per AI in lactating dairy cows. J. Dairy Sci., 91(3), 1044-1052.
• Brusveen DJ., Souza AH., Wiltbank MC., 2009. Effects of additional prostaglandin F2α and estradiol-17β during Ovsynch in lactating dairy cows. J. Dairy Sci., 92, 1412–1422.
• Carvalho NAT., Gimenes LU., Soares JG., Souza DC., Vannucci FS., Maio JRG., Baruselli PS., 2010. Delay of the Last GnRH Administration at the Ovsynch Protocol in Buffaloes. Revista Veterinaria., 21(1), 179.
• Çevik M., Selçuk M., Doğan S., 2010. Comparison of pregnancy rates after timed artificial insemination in Ovsynch, Heatsynch and CIDR-Based synchronization protocol in dairy cows. Kafkas Univ. Vet. Fak. Derg., 16(1), 85-89.
• Dogruer G., Saribay MK., Karaca F., Ergun Y., 2010. The comparison of the pregnancy rates obtained after the Ovsynch and double dose PGF2α+GnRH applications in lactating dairy cows. J. Anim. Vet. Adv., 9(4), 809-813.
• Erdem H., Güzeloğlu A., 2008. Holstein ırkı düvelerde sabit zamanlı tohumlama amacıyla iki farklı östrus ve senkronizasyon yönteminin değerlendirilmesi. Vet. Bil. Derg., 24(1), 7-13.
• Grant E., 2005: Estrous synchronization programs that help rebreed anestrous cows. Wahoo Productions Inc, 905 E. Brittany Way, Highlands Ranch, CO 80126. http://www.naab-css.org/education/timing.html. [Erişim: 17.02.1014].
• Heersche G., Nebel RL., 1994. Measuring efficiency and accuracy of detection of estrus. J. Dairy Sci., 77, 2754–2761.
• Jemmeson A., 2000. Synchronising ovulation in dairy cows with either two treatments of gonadotropin-releasing hormone and one of prostaglandin, or two treatments of prostaglandin. A.V.J., 78(2), 108–111.
• Kara U., Ayaşan T., Hızlı H., Gok K., 2011. Effect of Ovsynch Protocol on Pregnancy Rate in Heifers and Cows. Erciyes Üniv. Vet. Fak. Derg., 8(1), 1-8.
• McDougall S., Cullum AA., Anniss FM., FM Rhodes., 2001. Treatment of anovulatory anoestrous postpartum dairy cows with a gonadotropin-releasing hormone (GnRH), prostaglandin F2α, GnRH regimen or with progesterone and oestradiol benzoate. N.Z.V.J., 49(5), 168-172.
• Moreira F., Risco CA., Pires MFA., Ambrose JD., Drost M., and Thatcher WW., 2000. Use of bovine somatotropin in lactating diary cows receiving timed artificial insemination. J. Dairy Sci., 83, 1237–1247.
• Navanukraw C., Redmer DA., Reynolds LP., Kirsch JD., Grazul-Bilska AT., Fricke PM., 2004. A modified presynchronization protocol improves fertility to timed artificial insemination in lactating dairy cows. J. Dairy Sci., 87, 1551-1557.
• Nebel RL., Jobst SM., 1998. Evalution of systematic breeding programs for lactating dairy cows: A review, J, Dairy Sci., 81, 1169–1174.
• O’Connor ML., 2001. Estrus synchronization programs for the dairy herd. Dairy and Anim. Sci., DAS 01-35.
• O’Connor ML., 2005. Systematic breeding program for dairy cows. Dairy and Anim. Sci., DAS 2005-99.
• Peeler ID., Nebel RL., Pearson RE., Swecker WS., Garcia A., 2004. Pregnancy rates after timed AI of heifers following removal of intravaginal progesterone inserts. J. Dairy Sci., 87, 2868–2873.
• Peters AR., Mawhinney I., Drew SB., Ward SJ., Warren MJ. and Gordon PJ., 1999. Development of a gonadotrophin-releasing hormone and prostaglandin regimen for the planned breeding of dairy cows. Vet. Rec. 145: 516–521.
• Peters MW., Pursley JR., 2002. Fertility of lactating dairy cows treated with ovsynch after presynchronization injections of PGF2α and GnRH. J. Dairy Sci., 85, 2403-2406.
• Pursley JR., Mee MO., Wiltbank MC., 1995. Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology., 44, 915-923.
• Pursley JR., Silcox RW., Wiltbank MC., 1998. Effect of time of artificial insemination on pregnancy rates, calving rates, pregnancy loss, and gender ratio after synchronization of ovulation in lactating dairy cows. J. Dairy Sci., 81, 2139–2144.
• Pursley JR., Wiltbank MC., Stevenson JS., Ottobre JS., Garverick HA., Anderson LL.. 1997. Pregnancy rates per artificial insemination for cows and heifers inseminated at a synchronized ovulation or synchronized estrus. J. Dairy Sci., 80, 295-300.
• Quaife T., 1995. Targeted breeding. Dairy Herd Management., 24–25.
• Radostits OM., Leslieand KE., Fetrow J., 1994. Herd Health-Food Animal Production Medicine. 2nd ed. W.B. Saunders Co., Philadelphia, PA.
• Semacan A., Pancarcı ŞM., 2013. Üremenin denetlenmesi. In: Çiftlik Hayvanlarında Doğum ve Jinekoloji. Ed: Semacan A., Kaymaz M., Fındık M., Rişvanlı A., Köker A. Medipres Matbaacılık Ltd Şti, Malatya, pp: 99-124.
• Senger PL., 1994. The estrus detection problem: New Concepts, Technologies and Possibilities. J. Dairy Sci., 77, 2745–2753.
• Souza, AH., Gumen A., Silva EPB., Cunha AP., Guenther JN., Peto CM., Caraviello DZ., Wiltbank MC., 2007. Supplementation with estradiol-17β before the last GnRH of the Ovsynch protocol in lactating dairy cows. J. Dairy Sci., 90, 4623–4634.
• Stevenson, JS., Kobayashi Y., Shipa MP., Rauchholz KC., 1996. Altering conception of dairy cattle by gonadotropin-releasing hormone preceding luteolysis induced by prostaglandin F2α. J. Dairy Sci., 79, 402–410.
• Tenhagen BA., Surholt R., Wittke M., Vogel C., Drillich M., Heuwieser W., 2004. Use of Ovsynch in dairy herds-differences between primiparous and multiparous cows. Anim. Reprod. Sci., 81, 1–11.
• Yılmaz C., Yılmaz O., Ucar M., 2011. Effect of PGF2α and GnRH ınjections applied before ovsynch on pregnancy rates in cows and heifers. Kafkas Univ. Vet. Fak. Derg., 17(4), 641-644.
• Yilmazbas-mecitoglu G., Karakaya E., Keskin A., Alkan A., Gumen A., 2013. Reducing the duration between gonadotropin-releasing hormone (GnrH) and prostaglandin F2α treatment in the Ovsynch protocol to 6 days improved ovulation to second GnrH treatment, but inclined to reduce fertility. J. Dairy Sci., 96, 3817–3824.