Glutamat Eksitotoksisitesi Oluşturulan Primer Kortikal Nöron Kültürlerinde Parietinin Nöroprotektif Etkisinin İncelenmesi

Year 2018, Volume: 13 Issue: 2, 165 - 173, 25.10.2018

Gülşah Gündoğdu , Ali Taghizadehghalehjoughi Betül Çiçek Onur Şenol , Kemal Alp Nalcı Alper Kürşat Demirkaya , Ahmet Hacımüftüoğlu

https://doi.org/10.17094/ataunivbd.363858

Cited By: 2

Abstract

Glutamat merkezi sinir sisteminin en önemli nörotransmitter maddesidir. Aşırı glutamat salınımı, glutamat reseptörlerinin

uzun süreli aktivasyonuna neden olarak eksitotoksisteye yol açar. Parietin, Rheum ribes L.’den izole edilen çeşitli farmakolojik

özelliklere sahip bir antrakinondur. Bu çalışmada glutamat eksitotoksisitesine maruz bırakılan primer kortikal nöron

kültürlerinde parietinin nöroprotektif etkisinin araştırılması amaçlanmıştır. Primer kortikal nöronlar, yeni doğan Sprague

Dawley cinsi sıçanlardan elde edildi. Glutamat eksitotoksisitesini stimüle etmek için, kültür medyumuna 10-5 M

konsantrasyonda glutamat uygulandı. Daha sonra hücrelere 2.5-500 μM konsantrasyonda parietin uygulanarak 24 saat

inkübasyona bırakıldı. Hücre canlılık oranı MTT yöntemi ile belirlendi. Aynı zamanda hücrelerde oluşan reaktif oksijen türleri

total antioksidan seviyesi (TAS)- total oksidan seviyesi (TOS) yöntemi ile değerlendirildi. MTT analiz sonuçlarına göre 10 μM

parietinin glutamat eksitotoksisitesine karşı nöronlarda anlamlı düzeyde koruyucu etkiye sahip olduğu tespit edildi. TAS-TOS

analiz sonuçlarına göre 10 μM parietinin hücrelerde antioksidan seviyesini anlamlı ölçüde artırırken, parietinin yüksek

konsantrasyonlarının hücrelerdeki oksidan seviyesini anlamlı ölçüde arttırdığı gözlendi. Bu çalışma sonucuna göre parietinin

primer kortikal nöron hücrelerinde glutamat eksitotoksisitesine karşı koruyucu etkiye sahip olduğu ve glutamat

eksitotoksisitesine karşı teropatik bir ajan olarak kullanılabileceği düşünülmektedir.

Keywords

Glutamat, Parietin, Primer nöron kültürü

Investigation of Protective Effect of Parietin Against Glutamate Excitotoxicity in Primary Cortical Neuron Culture

Abstract

Glutamate is the most important neurotransmitter in the central nervous system. Excitotoxicity is induced by

excessive release of glutamate followed by overstimulation of glutamate receptor. Parietin is an anthraquinone from Rheum

ribes L. and has been reported to have a variety of pharmacological properties. The present study investigated the

neuroprotective effects of parietin in primary cortical neuron cultures against glutamate excitotoxicity. Primary rat cortical

neuronal cultures were obtained from new born Sprague Dawley rats. Cultures were subject to 10-5 M glutamate to stimulate

glutamate excitotoxicity. After that, cells were treated with 2.5-500 μM concentrations of parietin during 24 h in dose

dependent manner. Cell viability was determined using MTT assay. Reactive oxygen species generation was assessed using

the total antioxidant status (TAS)-total antioxidant status (TOS) assays. The results of MTT analysis showed that 10 μM parietin

effectively protected neuron from glutamate toxicity. According to the results of TAS-TOS analysis, it showed that 10 μM

parietin significantly increased the antioxidant level in the cells, whereas high concentrations of parietin significantly

increased the oxidant level in the cells. The results of this study suggest that parietin had neuroprotective effect against

glutamate excitotoxicity in primary rat cortical neuron cultures and it may be conceive that parietin can be used as a

therapeutic agent for glutamate excitotoxicity.

Keywords

Glutamate, Parietin, Primary neuron culture

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