Abstract
An experiment was carried out to determine the effects of using DL-methionine hydroxy analog 2-Hydroxy-4-
(Methylthio) Butanoic acid (HMB) in broiler diets instead of methionine on broiler performance. Totally 256 female and male
equal number of broiler chicks were used. A total of 4 groups, 1 control and 3 experimental group, were formed which
contains 64 chicks in each of them. Each group divided into 4 subgroups which contains 16 equal numbers of female and male
chicks. Rations were corn-soybean meal based. The 0.20% DL-methionine was added to control diet and HMB added to other
three treatment groups 0.15, 0.20 and 0.25%, respectively. At the end of experiment there were no statistically differences
observed in live weight, feed consumption, live weight gain and feed efficiency (P>0.05). However, although the live weight
of the group that consumed 0.20% HMB and the control group were very close to each other, the live weight was lower about
55 g at the experimental groups consumed 0.15% and 0.25% HMB. These data shows that HMB which is DL-methionine
hydroxy analog can be used as an alternative diet supplement to DL-methionine in broiler diets.
References
- 1. AOAC., 1990. Official Methods of Analysis, 15. Ed., Association of Official Analytical Chemists, Arlington, VA. 2. Benevenga NJ., Fuller MF., Lall SP., McCracken KJ., Axford RFE., Omed HM., Phillips CJC., 2004. The Encyclopedia of Farm Animal Nutrition. CABI Publishing, UK. 3. Dibner JJ., 1983. Utilization of Supplemental Methionine Sources by Primary Cultures of Chick Hepatocytes. J Nutr, 113, 2216 – 2223. 4. Dibner JJ., Durley RC., Kostelc JG., Ivey EJ., 1990. 2-Hydroxy-4-(Methylthio) Butanoic Acid is a Naturally Occurring Methionine Precursor in the Chick. J Nutr, 120, 553 – 560. 5. Dibner JJ., Knight CD., 1984. Conversion of 2-Hydroxy-4-(Methylthio) Butanoic Acid to L-Methionine in the Chick: A Stereospecific Pathway. J Nutr, 114, 1716 – 1723. 6. Dibner JJ., Vazquez-Anon M., Parker D., Gonzalez-Esquerra R., Yi IG., Knight, CD., 2004. Use of Alimet Feed Supplement (2-Hydroxy-4(Methylthio) Butanoic Acid, HMTBA) for Broiler Production. J Poultry Sci, 41, 213 – 222. 7. Dikicioğlu T., Ergün A., Saçaklı P., 1997. Broyler Rasyonlarında Sıvı Metiyonin Kullanımı. Ankara Üniv Vet Fak Derg, 44, 237 – 248. 8. Drew MD., Van Kesel AG., Maenz DD., 2003. Absorption of Methionine and 2-Hydroxy-4-Methylthiobutoanic Acid in Conventional and Germ-Free Chickens. J Poultry Sci, 82, 1149 –1153. 9. Dupuis L., Saunderson CL., Puigserver A., Brachet, P., 1989. Oxidation of methionine and 2-hydroxy 4-methylthio butanoic acid stereoisomers in chicken tissues. Brit J Nutr, 62(1), 63-75 10. Garlich JD., 1985. Response of broilers to DL-methionine hydroxy analog free acid, DL-methionine, and L-methionine. J Poultry Sci, 64, 1541 – 1548. 11. Jankowski J., Ognik K., Kubinska M., Czech A., Juskiewicz J., Zdunczyk Z., 2017. The effect of DL-, L-isomers and DL-hydroxy analog administered at 2 levels as dietary sources of methionine on the metabolic and antioxidant parameters and growth performance of turkeys. Poultry Sci, 96, 3229-3238. 12. Kluge H., Gessner DK., Herzog E., Eder K., 2016. Efficacy of DL-methionine hydroxy analogue-free acid in comparison to DL-methionine in growing male white Pekin ducks. Poultry Sci, 95, 590-594. 13. Kratzer DD., Littell RC., 2006. Appropriate Statistical Methods to Compare Dose Responses of Methionine Sources. J Poultry Sci, 85, 947–954. 14. Lemme A., Hoehler D., Brennan JJ., Mannion PF., 2002. Relative Effectiveness of Methionine Hydroxy Analog Compared to DL-Methionine in Broiler Chickens. J Poultry Sci, 81, 838 – 845. 15. Martin-Venegas R., Geraert PA., Ferrer R., 2006. Conversion of the Methionine Hydroxy Analogue DL-2-Hydroxy-(4-Methylthio) Butanoic Acid to Sulfur-Containing Amino Acids in the Chicken Small Intestine. J Poultry Sci, 85, 1932 – 1938. 16. Ribeiro AML., Penz AM., 2001. Effects of 2-Hydroxy-4-(Methylthio)Butanoic Acid and DL-methionine on Broiler Performance and Compensatory Growth After Exposure To Two Different Environmental Temperatures. J Appl Poultry Res, 10, 419 – 426. 17. Richards JD., Atwell CA., Vazquez-Anon M., Dipner JJ., 2005. Comparative In Vitro and In Vivo Absorption of 2-Hydroxy-4(Methylthio) Butanoic Acid and Methionine in the Broiler Chicken. J Poultry Sci, 84, 1397 – 1405. 18. Salary J., Kalantar M., Dashtbin F., Hemati Matin HR., 2015. ALIMET (liquid methionine hydroxy analogue) in broiler chicken diets : immunity system, microflora population, and performance. Arch Zootec, 64(245), 57 – 62. 19. Sangali CP., Bruno LDG., Nunes RV., Neto ARO., Pozza PC., Oliveira MM.,Frank R., Schöne RA., 2014. Bioavailability of different methionine sources for growing broilers. R Bras Zootec, 43(3), 140-145 20. SAS, 2002. SAS user’s guide: Statistics, Version 11.5, Sas Institute, Cary. 21. Saunderson CL., 1985. Comparative metabolism of L-methionine, DL-methionine and DL-2-hydrox4y-methylthio butanoic acid by broiler chicks. Brit J Nutr, 54, 621 – 633. 22. Tang X., Zhao Y., Le G., Shi Y., Sun J., 2016. Effects of methionine hydroxy analogue on intestinal function and oxidative status in broiler chickens. The Experimental Biology 2016 Meeting, San Diego, Syf. 232. 23. TSE, 1991. Hayvan Yemleri. Metabolik (Cevrilebilir) Enerji Tayini (Kimyasal Metot). Türk Standartları Enstitüsü, TSE No: 9610, Aralık, Ankara. 24. Wang S., Bottje WG., Song Z., Beers K., Vazques-Anon M., Dipner JJ., 2001. Uptake of DL-2-Hydroxy-4-methylthio-butanoic Acid (DL-HMB) in the Broiler Liver In Vivo. J Poultry Sci, 80, 1619 – 1624. 25. Zhang S., Saremi B., Gilbert ER., Wong EA., 2017. Physiological and biochemical aspects of methionine isomers and a methionine analogue in broilers. Poultry Sci, 96, 425-439.
Abstract
Araştırma, etlik piliç yemlerinde metiyonin yerine metiyonin hidroksi analoğu olan 2-hidroksi-4-(metiltiyo) butanoik asidin
(HMB) kullanımının etlik piliç performansı üzerine etkilerini belirlemek amacıyla yapılmıştır. Toplam 256 adet eşit sayıda dişi
ve erkek etlik civciv kullanılmıştır. Her birinde 64 adet civciv bulunan 1 kontrol ve 3 deneme grubu olmak üzere toplam 4 grup
oluşturulmuştur. Her bir grup, dişi erkek sayısı eşit olan 16’ şar civcivden oluşan 4 alt gruba bölünmüştür. Yemler mısır ve soya
fasulyesi küspesi bazlı hazırlanmıştır. Kontrol grubunun yemine % 0.20 düzeylerinde DL-metiyonin, diğer 3 deneme grubuna
sırasıyla %0.15, 0.20 ve 0.25 düzeyinde HMB ilavesi yapılmıştır. Çalışma sonunda canlı ağırlık, yem tüketimi, canlı ağırlık artışı,
yemden yararlanma bakımından istatistikî farklılık olmadığı saptanmıştır (P>0.05). Ancak % 0.20 düzeyinde HMB içeren yemi
tüketen grup ile kontrol grubunun canlı ağırlıkları birbirine çok yakın olmasına karşın % 0.15 ve 0.25 HMB içeren yemi tüketen
deneme gruplarında çalışma sonu canlı ağırlık 55 g kadar daha düşük olmuştur. Bu veriler metiyonin hidroksi analoğu olan
HMB’in etlik piliç yemlerinde DL-metiyonine alternatif yem katkı maddesi olarak yerine kullanılabileceğini göstermektedir.
References
- 1. AOAC., 1990. Official Methods of Analysis, 15. Ed., Association of Official Analytical Chemists, Arlington, VA. 2. Benevenga NJ., Fuller MF., Lall SP., McCracken KJ., Axford RFE., Omed HM., Phillips CJC., 2004. The Encyclopedia of Farm Animal Nutrition. CABI Publishing, UK. 3. Dibner JJ., 1983. Utilization of Supplemental Methionine Sources by Primary Cultures of Chick Hepatocytes. J Nutr, 113, 2216 – 2223. 4. Dibner JJ., Durley RC., Kostelc JG., Ivey EJ., 1990. 2-Hydroxy-4-(Methylthio) Butanoic Acid is a Naturally Occurring Methionine Precursor in the Chick. J Nutr, 120, 553 – 560. 5. Dibner JJ., Knight CD., 1984. Conversion of 2-Hydroxy-4-(Methylthio) Butanoic Acid to L-Methionine in the Chick: A Stereospecific Pathway. J Nutr, 114, 1716 – 1723. 6. Dibner JJ., Vazquez-Anon M., Parker D., Gonzalez-Esquerra R., Yi IG., Knight, CD., 2004. Use of Alimet Feed Supplement (2-Hydroxy-4(Methylthio) Butanoic Acid, HMTBA) for Broiler Production. J Poultry Sci, 41, 213 – 222. 7. Dikicioğlu T., Ergün A., Saçaklı P., 1997. Broyler Rasyonlarında Sıvı Metiyonin Kullanımı. Ankara Üniv Vet Fak Derg, 44, 237 – 248. 8. Drew MD., Van Kesel AG., Maenz DD., 2003. Absorption of Methionine and 2-Hydroxy-4-Methylthiobutoanic Acid in Conventional and Germ-Free Chickens. J Poultry Sci, 82, 1149 –1153. 9. Dupuis L., Saunderson CL., Puigserver A., Brachet, P., 1989. Oxidation of methionine and 2-hydroxy 4-methylthio butanoic acid stereoisomers in chicken tissues. Brit J Nutr, 62(1), 63-75 10. Garlich JD., 1985. Response of broilers to DL-methionine hydroxy analog free acid, DL-methionine, and L-methionine. J Poultry Sci, 64, 1541 – 1548. 11. Jankowski J., Ognik K., Kubinska M., Czech A., Juskiewicz J., Zdunczyk Z., 2017. The effect of DL-, L-isomers and DL-hydroxy analog administered at 2 levels as dietary sources of methionine on the metabolic and antioxidant parameters and growth performance of turkeys. Poultry Sci, 96, 3229-3238. 12. Kluge H., Gessner DK., Herzog E., Eder K., 2016. Efficacy of DL-methionine hydroxy analogue-free acid in comparison to DL-methionine in growing male white Pekin ducks. Poultry Sci, 95, 590-594. 13. Kratzer DD., Littell RC., 2006. Appropriate Statistical Methods to Compare Dose Responses of Methionine Sources. J Poultry Sci, 85, 947–954. 14. Lemme A., Hoehler D., Brennan JJ., Mannion PF., 2002. Relative Effectiveness of Methionine Hydroxy Analog Compared to DL-Methionine in Broiler Chickens. J Poultry Sci, 81, 838 – 845. 15. Martin-Venegas R., Geraert PA., Ferrer R., 2006. Conversion of the Methionine Hydroxy Analogue DL-2-Hydroxy-(4-Methylthio) Butanoic Acid to Sulfur-Containing Amino Acids in the Chicken Small Intestine. J Poultry Sci, 85, 1932 – 1938. 16. Ribeiro AML., Penz AM., 2001. Effects of 2-Hydroxy-4-(Methylthio)Butanoic Acid and DL-methionine on Broiler Performance and Compensatory Growth After Exposure To Two Different Environmental Temperatures. J Appl Poultry Res, 10, 419 – 426. 17. Richards JD., Atwell CA., Vazquez-Anon M., Dipner JJ., 2005. Comparative In Vitro and In Vivo Absorption of 2-Hydroxy-4(Methylthio) Butanoic Acid and Methionine in the Broiler Chicken. J Poultry Sci, 84, 1397 – 1405. 18. Salary J., Kalantar M., Dashtbin F., Hemati Matin HR., 2015. ALIMET (liquid methionine hydroxy analogue) in broiler chicken diets : immunity system, microflora population, and performance. Arch Zootec, 64(245), 57 – 62. 19. Sangali CP., Bruno LDG., Nunes RV., Neto ARO., Pozza PC., Oliveira MM.,Frank R., Schöne RA., 2014. Bioavailability of different methionine sources for growing broilers. R Bras Zootec, 43(3), 140-145 20. SAS, 2002. SAS user’s guide: Statistics, Version 11.5, Sas Institute, Cary. 21. Saunderson CL., 1985. Comparative metabolism of L-methionine, DL-methionine and DL-2-hydrox4y-methylthio butanoic acid by broiler chicks. Brit J Nutr, 54, 621 – 633. 22. Tang X., Zhao Y., Le G., Shi Y., Sun J., 2016. Effects of methionine hydroxy analogue on intestinal function and oxidative status in broiler chickens. The Experimental Biology 2016 Meeting, San Diego, Syf. 232. 23. TSE, 1991. Hayvan Yemleri. Metabolik (Cevrilebilir) Enerji Tayini (Kimyasal Metot). Türk Standartları Enstitüsü, TSE No: 9610, Aralık, Ankara. 24. Wang S., Bottje WG., Song Z., Beers K., Vazques-Anon M., Dipner JJ., 2001. Uptake of DL-2-Hydroxy-4-methylthio-butanoic Acid (DL-HMB) in the Broiler Liver In Vivo. J Poultry Sci, 80, 1619 – 1624. 25. Zhang S., Saremi B., Gilbert ER., Wong EA., 2017. Physiological and biochemical aspects of methionine isomers and a methionine analogue in broilers. Poultry Sci, 96, 425-439.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Araştırma Makaleleri |
| Authors | |
| Publication Date | December 25, 2018 |
| Published in Issue | Year 2018 Volume: 13 Issue: 3 |