Research Article
The Effect of Lycopene Application on the Antioxidant Activity in Liver and Kidney Tissues of Diabetic Rats
Abstract
The scope of the present study was to determine the effect of lycopene on the activity of malondialdehyde (MDA), glutathione, vitamin A, vitamin E and catalase in liver and kidney tissues of diabetic rats. Wistar albino male rats were randomly allocated into four groups: control, diabetic, diabetic+lycopene and lycopene group (n=7). Rats in the respective groups were treated with intraperitoneal streptozotocin (45 mg/kg) to induce diabetes. Rats in the group lycopene and diabetic+lycopene were given orally 1 ml lycopene every day for 1 month. Catalase levels in the liver tissues of lycopene group was significantly lower than other groups (P<0.05). A significant decrease was observed at Vitamin A levels in the kidney tissues of the groups diabetic, diabetic+lycopene and lycopene compared to the control group (P<0.05). Other non-significant differences between the groups except catalase levels in liver tissue and vitamin A levels in kidney tissues may be result of a short period of diabetes mellitus. The results of the present study supported the positive correlation among metabolic control and oxidative stress in diabetes mellitus.
References
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Selçuk Üniv Tıp Fak Biyokimya Anabilim Dalı, Uzmanlık Tezi, Konya. 9. Blokhina O., Virolainen E., Fagerstedt KV., 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot Fennici, 91, 179-194. 10. Martinez A., Rodriguez-Girones MA., Barbosa A., Costas M., 2008. Donator acceptor map for carotenoids, melatonin and vitamins. J Phys Chem A, 112, 9037-9042. 11. Lauretani F., Semba RD., Dayhoff-Brannigan M., Corsi AM., Di Iorio A., Buiatti E., Bandinelli S., Guralnik JM., Ferrucci L., 2008. Low total plasma carotenoids are independent predictors of mortality among older persons: The In CHIANTI study. Eur J Nutr, 47, 335-340. 12. Omoni AO., Aluko RE., 2005. The anti-carcinogenic and anti-athero- genic effects of lycopene: a review. Trends Food Sci Tech, 16, 344-350. 13. Amin AR., Kucuk O., Khuri FR., Shin DM., 2009. Perspectives for cancer prevention with natural compounds. J Clin Oncol, 27, 2712-2725. 14. Ellinger S., Ellinger J., Müller SC., Stehle P., 2009. Tomatoes and lycopene in prevention and therapy-is there an evidence for prostate diseases? Aktuelle Urol, 40, 37-43. 15. Gupta SK., Trivedi D., Srivastava S., Joshi S., Halder N., Verma SD., 2003. Lycopene attenuates oxidative stress induced experimental cataract development: an in vitro and in vivo study. Nutrition, 19, 794-799. 16. Karabay G., Zagyapan R., Take G., 2006. Streptozotosinle oluşturulan diabetin sıçan periferik sinirleri üzerine etkisinin elektron mikroskobik incelenmesi. Uludag Uni Tıp Fak Derg, 32, 77-81. 17. Rencuzogullari N., Erdogan S., 2007. Oral administration of lycopene reverses cadmium-suppressed body weight loss and lipid peroxidation in rats. Biol Trace Elem Res, 118, 175-183. 18. Beutler E., Duran O., Kelly B., 1963. Improved method for the determination of blood glutathione. J Lab Clin Med, 61, 882-888. 19. Martinek RG., 1964. Method for the determination of Vitamin E (total tocopherols) in serum. Clin Chem, 10, 1078-1086. 20. Suzuki I., Katoh N., 1990. A simple and cheap methods for measuring serum vitamin A in cattle using only a spectrophotometer. Jpn J Vet Sci, 52, 1281-1283. 21. Gutteridge JM., 1995. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem, 41, 1819-1828. 22. Aebi H., 1984. Catalase in Vitro. Methods Enzymol, 105, 121-126. 23. Memisogullari R., Bakan E., 2004. Levels of ceruloplasmin, transferrin, and lipid peroxidation in the serum of patients with Type 2 diabetes mellitus. J Diabetes Complicat, 18, 193-197. 24. Ramachandran V., Saravanan R., 2013. Asiatic acid prevents lipid peroxidation and improves antioxidant status in rats with streptozotocin- induced diabetes. J Funct Food, 5, 1077-1087. 25. Saravanan G., Ponmurugan P., 2011. Ameliorative potential of S-allyl cysteine on oxidative stress in STZ induced diabetic rats. Chem-Biol Interact, 189, 100-106. 26. Singh S., Melkani GC., Rani C., Gaur SPS., Agrawal V., Agrawal CG., 1997. Oxidative stres and metabolic control in non-insulin dependent diabetes mellitus. Indian J Biochem Bio, 34, 512-517. 27. Wierusz-Wysocka B., Wysocki H., Byks H., Zozulinska D., Wykretowicz A., 1995. Metabolic control quality and free radical activity in diabetic patients. Diabetes Res Clin Pract, 27, 193-197. 28. Yılmaz N., Vural H., Eren Z., Ceylan C., Nazlıgül Y., 2000. Tip 2 diyabetik hastalarda diyabet süresinin oksidatif stres üzerine etkisi. Türk Tıp Derg, 7, 37-39. 29. Ciftci Yegin S., Mert N., 2013. Deneysel Olarak Diyabet Oluşturulmuş Sıçanlarda Hba1c, Mda, Gsh-Px ve Sod Miktarlarının Tayini. Van Vet J, 24, 51-54. 30. Maritim AC., Sanders RA., Watkins JB., 2003. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxic, 17, 24-38. 31. Demir E., Yılmaz Ö., 2014. Streptozotosinin neden olduğu tip-1 diyabette cam yağının karaciğer ve böbrek dokusundaki bazı biyokimyasal parametrelere etkisi. Karaelmas Fen Müh Derg, 4, 43-51. 32. Memisogullari R., Taysi S., Bakan E., Capoglu I., 2003. Antioxidant Status and Lipid Peroxidation Type II Diabetes Mellitus. Cell Biochem Funct, 21, 291-296. 33. Komosinska-Vassev K., Olczyk K., Olczyk P., Winsz-Szczotka K., 2005. Effects of metabolic control and vascular complications on indices of oxidative stress in type 2 diabetic patients. Diabetes Res Clin Pr, 68, 207-216. 34. Salvi J., Matabosch C., Fofi D., Forest J., 2007. A review of recent range image registration methods with accuracy evaluation. Image Comput, 25, 578-596. 35. Memisogulları R., 2005. Diyabette serbest radikallerin rolü ve antioksidanların etkisi. Düzce Tıp Fak Derg, 3, 30-39. 36. Cao U., Dc E., In U., Ac N., 2006. Effect of glycaemic control on serum retinol and beta carotene levels in Type 2 diabetics in Calabar, Nigeria. Malays J Nutr, 12, 55-65. 37. Tsin ATC., Griffin BW., Mata NL., Yu HS., Williams GW., Crider JY., Chandler ML., 1993. Vitamin A homeostasis in the diabetic rat. J Clin Biochem Nutr, 15, 23-31. 38. Cemek M., Kaga S., Simsek N., Buyukokuroglu ME., Konuk M., 2008. Antihyperglycemic and antioxidative potential of Matricaria chamomilla L. in streptozotocin induced diabetic rats. J Nat Med, 62, 284-293. 39. Miyazaki H., Takitani K., Koh M., Takaya R., Yoden A., Tamai H., 2013. α-tocopherol status and expression of α-tocopherol transfer protein in type 2 diabetic Goto-Kakizaki rats. J Nutr Sci Vitaminol, 59, 64-68. 40. Hozumi M., Murata T., Morinobu T., Manago M., Kuno T., Tokuda M., Konishi K., Mingci Z., Tamai H., 1998. Plasma beta-carotene, retinol, and alpha-tocopherol levels in relation to glycemic control of children with insulindependent diabetes mellitus. J Nutr Sci Vitaminol, 44, 1-9. 41. Arulselvan P., Subramanian SP., 2007. Beneficial effects of Murraya koenigii leaves on antioxidant defense system and ultra structural changes of pancreatic β-cells in experimental diabetes in rats. Chem.-Biol. Interact, 165, 155-164. 42. Noyan T., Balahoroglu R., Komuroglu U., 2004. Diyabetik sıçanlarda ünsülinle kombine edilmiş A, E ve C vitamini tedavisinin antioksidan enzimler üzerine Etkileri. Turkiye Klinikleri J Med Sci, 2, 113-119. 43. Moreira EAM., Fagundes RLM., Filho DW., Neves D., Sell F., Bellisle F., Kupek E., 2005. Effects of diet energy level and tomato powder comsumption on antioxidant status in rats. Clin Nutr, 24, 1038-1046. 44. Nix WA., Zirwes R., Bangert V., Kaiser RP., Schilling M., Hostalek U., Obeid R., 2015. Vitamin B status in patients with type 2 diabetes mellitus with and without incipient nephropathy. Diabetes Res Clin Pract, 107, 157-165. 45. Yan MKW., Khalil H., 2017. Vitamin supplements in type 2 diabetes mellitus management: A review. Diabetes Metab Syndr, 11, 589-595 46. Adedara IA., Awogbindin IO., Anamelechi JP., Farombi EO., 2015. Garcinia kola seed ameliorates renal, hepatic, and testicular oxidative damage in streptozotocin-induced diabetic rats. Pharma Biol, 53,695-704. 47. Mushtaq N., Schmatz R., Ahmed M., Pereira LB., Costa P., Reichert KP., Dalenogare D., Pelinson LP., Vieira JM., Stefanello N., Oliveira LS., Mulinacci N., Bellumori M., Morsch VM., Maria Rosa Schetinger MR., 2015. Protective effect of rosmarinic acid against oxidative stress biomarkers in liver and kidney of strepotozotocin-induced diabetic rats. J Physiol Biochem, 71, 743-751.
Diyabetik Ratların Karaciğer ve Böbrek Dokularındaki Antioksidan Aktivite Üzerine Likopen Uygulamasının Etkileri
Abstract
Bu çalışmanın amacı, diyabetik ratlarda likopen uygulamasının karaciğer ve böbrek dokusundaki malondialdehit, glutatyon, vitamin A, vitamin E ve katalaz üzerine etkilerini araştırmaktır. Çalışmada kullanılan Wistar cinsi albino erkek ratlar, içlerinden rastgele seçilerek kontrol grubu, diyabet grubu, likopen grubu ve diyabet+likopen grubu olmak üzere 4 farklı gruba ayrıldı. Her grupta 7 adet rat bulunmaktadır. Deneysel diyabet oluşturmak için diyabet grubu ve diyabet+likopen grubundaki ratlara intraperitonel yoldan streptozotosin (45 mg/kg) uygulandı. Diyabet+likopen grubu ile likopen grubundaki ratlara 1 ay boyunca her gün oral yoldan 1 ml likopen (ayçiçeği yağında eritildi) uygulandı. Likopen grubunun karaciğer dokularındaki katalaz düzeyi diğer gruplardan önemli derecede düşük bulundu (P<0.05). Diyabet, diyabet+likopen ve likopen gruplarının böbrek dokularındaki vitamin A düzeyleri diğer gruplarla kıyaslandığında istatistiksel olarak önemli bir azalış göstermiştir (P<0.05). Böbrek dokularındaki vitamin A düzeyleri ve karaciğer dokularındaki katalaz düzeyleri hariç diğer gruplar arasındaki istatistiksel olarak önemli olmayan farklılıkların olması Diabetes Mellitusun kısa periyodunun sonuçları olabileceği kanaatindeyiz. Çalışmanın sonucu olarak, yaptığımız araştırma Diabetes Mellitusta oksidatif stres ile metabolik kontrol arasındaki pozitif korelasyonu destekler niteliktedir.
References
- 1. Onat T., Emerk K., Sözmen YE., 2006. İnsan Biyokimyası. In “Diabetes Mellitus”, Ed. T Onat, K Emerk, EY Sözmen, 2nd ed., 280-281, Palme Yayıncılık, Ankara. 2. Di Mascio P., Murphy ME., Sies H., 1991. Antioxidant defense systems: the role of carotenoids, tocopherols, and thiols. Am J Clin Nutr, 53, 194-200. 3. Larson RA., 1988. The antioxidants of higher plants. Phytochemistry, 27, 969-978. 4. Kalaycıoğlu L., Serpek B., Nizamlıoğlu M., Başpınar N., Tiftik AM., 2000. Biyokimya, 265-305, 2nd ed., Nobel Yayın Dağıtım Ltd Şti, Ankara. 5. Murray RK., Granner DK., Mayes PA., Rodwell VW., 2004. Harper Biyokimya, 642-653, 25nd ed., Nobel Matbaacılık, Ankara. 6. Stringer MD., Gorog PG., Freeman A., Kaskar VV., 1989. Lipid peroxides and atherosclerosis. Br Med J, 298, 281-284. 7. Karabulut H., Gulay MŞ., 2016. Serbest radikaller. MAKÜ Sag Bil Enst Der, 4, 50-59. 8. Dikici İ., 1999. Akut viral hepatitlerle interferon tedavisi görmüş kronik viral hepatitlerde oksidatif stresin araştırılması. Selçuk Üniv Tıp Fak Biyokimya Anabilim Dalı, Uzmanlık Tezi, Konya. 9. Blokhina O., Virolainen E., Fagerstedt KV., 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot Fennici, 91, 179-194. 10. Martinez A., Rodriguez-Girones MA., Barbosa A., Costas M., 2008. Donator acceptor map for carotenoids, melatonin and vitamins. J Phys Chem A, 112, 9037-9042. 11. Lauretani F., Semba RD., Dayhoff-Brannigan M., Corsi AM., Di Iorio A., Buiatti E., Bandinelli S., Guralnik JM., Ferrucci L., 2008. Low total plasma carotenoids are independent predictors of mortality among older persons: The In CHIANTI study. Eur J Nutr, 47, 335-340. 12. Omoni AO., Aluko RE., 2005. The anti-carcinogenic and anti-athero- genic effects of lycopene: a review. Trends Food Sci Tech, 16, 344-350. 13. Amin AR., Kucuk O., Khuri FR., Shin DM., 2009. Perspectives for cancer prevention with natural compounds. J Clin Oncol, 27, 2712-2725. 14. Ellinger S., Ellinger J., Müller SC., Stehle P., 2009. Tomatoes and lycopene in prevention and therapy-is there an evidence for prostate diseases? Aktuelle Urol, 40, 37-43. 15. Gupta SK., Trivedi D., Srivastava S., Joshi S., Halder N., Verma SD., 2003. Lycopene attenuates oxidative stress induced experimental cataract development: an in vitro and in vivo study. Nutrition, 19, 794-799. 16. Karabay G., Zagyapan R., Take G., 2006. Streptozotosinle oluşturulan diabetin sıçan periferik sinirleri üzerine etkisinin elektron mikroskobik incelenmesi. Uludag Uni Tıp Fak Derg, 32, 77-81. 17. Rencuzogullari N., Erdogan S., 2007. Oral administration of lycopene reverses cadmium-suppressed body weight loss and lipid peroxidation in rats. Biol Trace Elem Res, 118, 175-183. 18. Beutler E., Duran O., Kelly B., 1963. Improved method for the determination of blood glutathione. J Lab Clin Med, 61, 882-888. 19. Martinek RG., 1964. Method for the determination of Vitamin E (total tocopherols) in serum. Clin Chem, 10, 1078-1086. 20. Suzuki I., Katoh N., 1990. A simple and cheap methods for measuring serum vitamin A in cattle using only a spectrophotometer. Jpn J Vet Sci, 52, 1281-1283. 21. Gutteridge JM., 1995. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem, 41, 1819-1828. 22. Aebi H., 1984. Catalase in Vitro. Methods Enzymol, 105, 121-126. 23. Memisogullari R., Bakan E., 2004. Levels of ceruloplasmin, transferrin, and lipid peroxidation in the serum of patients with Type 2 diabetes mellitus. J Diabetes Complicat, 18, 193-197. 24. Ramachandran V., Saravanan R., 2013. Asiatic acid prevents lipid peroxidation and improves antioxidant status in rats with streptozotocin- induced diabetes. J Funct Food, 5, 1077-1087. 25. Saravanan G., Ponmurugan P., 2011. Ameliorative potential of S-allyl cysteine on oxidative stress in STZ induced diabetic rats. Chem-Biol Interact, 189, 100-106. 26. Singh S., Melkani GC., Rani C., Gaur SPS., Agrawal V., Agrawal CG., 1997. Oxidative stres and metabolic control in non-insulin dependent diabetes mellitus. Indian J Biochem Bio, 34, 512-517. 27. Wierusz-Wysocka B., Wysocki H., Byks H., Zozulinska D., Wykretowicz A., 1995. Metabolic control quality and free radical activity in diabetic patients. Diabetes Res Clin Pract, 27, 193-197. 28. Yılmaz N., Vural H., Eren Z., Ceylan C., Nazlıgül Y., 2000. Tip 2 diyabetik hastalarda diyabet süresinin oksidatif stres üzerine etkisi. Türk Tıp Derg, 7, 37-39. 29. Ciftci Yegin S., Mert N., 2013. Deneysel Olarak Diyabet Oluşturulmuş Sıçanlarda Hba1c, Mda, Gsh-Px ve Sod Miktarlarının Tayini. Van Vet J, 24, 51-54. 30. Maritim AC., Sanders RA., Watkins JB., 2003. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxic, 17, 24-38. 31. Demir E., Yılmaz Ö., 2014. Streptozotosinin neden olduğu tip-1 diyabette cam yağının karaciğer ve böbrek dokusundaki bazı biyokimyasal parametrelere etkisi. Karaelmas Fen Müh Derg, 4, 43-51. 32. Memisogullari R., Taysi S., Bakan E., Capoglu I., 2003. Antioxidant Status and Lipid Peroxidation Type II Diabetes Mellitus. Cell Biochem Funct, 21, 291-296. 33. Komosinska-Vassev K., Olczyk K., Olczyk P., Winsz-Szczotka K., 2005. Effects of metabolic control and vascular complications on indices of oxidative stress in type 2 diabetic patients. Diabetes Res Clin Pr, 68, 207-216. 34. Salvi J., Matabosch C., Fofi D., Forest J., 2007. A review of recent range image registration methods with accuracy evaluation. Image Comput, 25, 578-596. 35. Memisogulları R., 2005. Diyabette serbest radikallerin rolü ve antioksidanların etkisi. Düzce Tıp Fak Derg, 3, 30-39. 36. Cao U., Dc E., In U., Ac N., 2006. Effect of glycaemic control on serum retinol and beta carotene levels in Type 2 diabetics in Calabar, Nigeria. Malays J Nutr, 12, 55-65. 37. Tsin ATC., Griffin BW., Mata NL., Yu HS., Williams GW., Crider JY., Chandler ML., 1993. Vitamin A homeostasis in the diabetic rat. J Clin Biochem Nutr, 15, 23-31. 38. Cemek M., Kaga S., Simsek N., Buyukokuroglu ME., Konuk M., 2008. Antihyperglycemic and antioxidative potential of Matricaria chamomilla L. in streptozotocin induced diabetic rats. J Nat Med, 62, 284-293. 39. Miyazaki H., Takitani K., Koh M., Takaya R., Yoden A., Tamai H., 2013. α-tocopherol status and expression of α-tocopherol transfer protein in type 2 diabetic Goto-Kakizaki rats. J Nutr Sci Vitaminol, 59, 64-68. 40. Hozumi M., Murata T., Morinobu T., Manago M., Kuno T., Tokuda M., Konishi K., Mingci Z., Tamai H., 1998. Plasma beta-carotene, retinol, and alpha-tocopherol levels in relation to glycemic control of children with insulindependent diabetes mellitus. J Nutr Sci Vitaminol, 44, 1-9. 41. Arulselvan P., Subramanian SP., 2007. Beneficial effects of Murraya koenigii leaves on antioxidant defense system and ultra structural changes of pancreatic β-cells in experimental diabetes in rats. Chem.-Biol. Interact, 165, 155-164. 42. Noyan T., Balahoroglu R., Komuroglu U., 2004. Diyabetik sıçanlarda ünsülinle kombine edilmiş A, E ve C vitamini tedavisinin antioksidan enzimler üzerine Etkileri. Turkiye Klinikleri J Med Sci, 2, 113-119. 43. Moreira EAM., Fagundes RLM., Filho DW., Neves D., Sell F., Bellisle F., Kupek E., 2005. Effects of diet energy level and tomato powder comsumption on antioxidant status in rats. Clin Nutr, 24, 1038-1046. 44. Nix WA., Zirwes R., Bangert V., Kaiser RP., Schilling M., Hostalek U., Obeid R., 2015. Vitamin B status in patients with type 2 diabetes mellitus with and without incipient nephropathy. Diabetes Res Clin Pract, 107, 157-165. 45. Yan MKW., Khalil H., 2017. Vitamin supplements in type 2 diabetes mellitus management: A review. Diabetes Metab Syndr, 11, 589-595 46. Adedara IA., Awogbindin IO., Anamelechi JP., Farombi EO., 2015. Garcinia kola seed ameliorates renal, hepatic, and testicular oxidative damage in streptozotocin-induced diabetic rats. Pharma Biol, 53,695-704. 47. Mushtaq N., Schmatz R., Ahmed M., Pereira LB., Costa P., Reichert KP., Dalenogare D., Pelinson LP., Vieira JM., Stefanello N., Oliveira LS., Mulinacci N., Bellumori M., Morsch VM., Maria Rosa Schetinger MR., 2015. Protective effect of rosmarinic acid against oxidative stress biomarkers in liver and kidney of strepotozotocin-induced diabetic rats. J Physiol Biochem, 71, 743-751.
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Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Araştırma Makaleleri |
| Authors | |
| Publication Date | October 25, 2019 |
| Published in Issue | Year 2019 Volume: 14 Issue: 2 |