Abstract
It is essential to demonstrate organs and their structures practically in anatomy education. Plastination is an anatomical technique that has been used to prepare biological specimens for educational purposes. it is a gradual process by replacement of fluids in biological tissues with reactive polymers such as silicone, epoxy or polyester resin. This study was aimed to plastinate the internal genital organs of adult cows by S10B silicone plastination method. Ten internal genital organs of cows were plastinated to be used in veterinary undergraduate teaching/practices. After dissection and fixation, the specimens were plastinated with S10B plastination method and colour differentiation of the organ parts were measured. The plastination process was completed with dehydration, defatting, impregnation, and gas curing stages, respectively. It was observed that the plastination of specimens with S10B plastination method preserve normal anatomy of genital organs. It was estimated that all parts of uterus became greenish and bluish colour and the value of the brightness of uterus was increased. The tissue shrinkage was reduced considerably when dehydration was carried out in a cold environment. It was concluded that S10B plastination method can be easily used to prepare the specimens of genital organs to be used in veterinary teaching.
References
- 1. Thibier M., Wagner HG., 2002. World statistics for artificial insemination in cattle. Livest Prod Sci, 74, 203-212. 2. Oto C., Bakici C., Insal B., Yilmaz B., Ozen D., 2018. Evaluation of the acceptability of fresh dog cadavers in anatomy education. Indian J Anim Res, 808, 1-5. 3. Ramos ML., De Paula TAR., Zerlotini MF., Silva VHD., Carazo LB., De Paula MF., Silva FFR., Santana ML., Silva LC., Ferreira LBC., 2018. A comparison of different de-plastination methodologies for preparing histological sections of material plastinated with Biodur® S10/S3. J Int Soc Plastination, 30, 10-15. 4. Sivagnanam S., Balasundaram K., Selvaraju M., Ravikumar K., Kumaravel A., Paramasivan S., 2014. The polyester resin plastination as a tool for making permanent dry specimens for teaching gynaecology. AJST, 5, 185-186. 5. Riederer BM., 2014. Plastination and its importance in teaching anatomy. Critical points for long-term preservation of human tissue. J Anat, 224, 309-315. 6. Estai M., Bunt S., 2016. Best teaching practices in anatomy education: A critical review. Ann Anat, 208, 151-157. 7. Ottone NE., Cirigliano V., Bianchi HF., Medan CD., Algieri RD., Brum GB., Fuentes R., 2015. New contributions to the development of a plastination technique at room temperature with silicone. Anat Sci Int, 90, 126-135. 8. Ottone NE., Del Sol M., Fuentes R., 2016. Report on a sheet plastination technique using commercial epoxy resin. Int J Morphol, 34, 1039-1043. 9. Ottone NE., Vargas CA., Veuthey C., Del Sol M., Fuentes, R., 2018. Epoxy sheet plastination on a rabbit head–new faster protocol with Biodur® E12/E1. Int J Morphol, 36, 441-446. 10. Steinke H., Rabi S., Saito T., Sawutti A., Miyaki T., Itoh M., Spanel-Borowski K., 2008. Light-weight plastination. Ann Anat, 190, 428-431. 11. Pashaei S., 2010. A brief review on the history, methods and applications of plastination. Int J Morphol, 28, 1075-1079. 12. Suganthy J., Francis DV., 2012. Plastination using standard S10 technique-Our experience in Christian Medical Collage, Vellore. J Anat Soc India, 61, 44-47. 13. Kumro SL., Crocker AV., Powell RL., 2013. Injection plastination: A low-tech, inexpensive method for silicone preservation of small vertebrates. J Int Soc Plastination, 25, 12-17. 14. Gonzalvez M., Ortiz J., Latorre R., 2017. S10 Plastination technique for preservation of parasites: the case of Oestrus ovis larvae. J Int Soc Plastination, 29, 5-10. 15. Latorre RM., Garcia-Sanz MP., Moreno M., Hernandez F., Gil F., Lopez O., Ayala MD., Ramirez G., Vazquez JM., Arencibia A., Henry RW., 2011. How useful is plastination in learning anatomy? JVME, 34, 172-176. 16. Chapman SJ., Hakeem AR., Marangoni G., Prasad KR., 2013. Anatomy in medical education: Perceptions of undergraduate medical students. Ann Anat, 195, 409-414. 17. Sivagnanam S., Balasundaram K., Selvaraju M., Ravikumar K., Kumaravel A., Paramasivan S., 2014. The epoxy resin plastination of reproductive organs of animals. AJST, 5, 181-182. 18. Brown MA., Reed RB., Henry RW., 2002. Effects of dehydration mediums and temperature on total dehydration time and tissue shrinkage. J Int Soc Plastination, 17, 28-33. 19. Ekim O., İnsal B., Bakıcı C., Hazıroğlu RM., Akgün RO., 2014a. Yılanlarda Soğuk Ortam Tekniği ile Tüm Vücut Silikon Plastinasyonu. Dicle Üniv Vet Fak Derg, 1, 1-14. 20. Ekim O., Tunalı S., Hazıroğlu RM., Ayvalı M., 2014. Evcil memeli hayvanlarda böbreklerin soğuk ortam tekniği ile silikon plastinasyonu. Vet Hekim Der Derg, 85, 1-11.
Abstract
Anatomi eğitimi sırasında, organları ve bu organların yapılarını uygulamalı olarak göstermek oldukça önemlidir. Plastinasyon, biyolojik örneklerin eğitim amacı ile hazırlanılarak kullanılmakta olan anatomik bir tekniktir. Biyolojik dokulardaki sıvıların silikon, epoksi veya polyester reçine gibi reaktif polimerler ile değiştirilmesi sonucunda gerçekleşen aşamalı bir işlemdir. Bu çalışmada, yetişkin ineklerin iç genital organlarının S10B silikon plastinasyon yöntemi ile plastine edilmesi amaçlanmıştır. Veteriner hekimlik lisans öğretimi ve uygulamalarında kullanılmak üzere 10 adet ineğe ait iç genital organlar plastine edildi. Diseksiyon ve tespit işleminden sonra, örneklere S10B plastinasyon yönteminin diğer önemli aşamaları uygulandı ve organ bölümlerine ait renk değişimleri kantitatif olarak hesaplandı. Plastinasyon işlemleri sırasıyla, dehidrasyon, yağdan arındırma, zorla impregnasyon ve gazla kürleme - sertleştirme aşamaları ile tamamlandı. S10B plastinasyon yöntemi ile plastine edilen örneklerin normal anatomik yapısını koruduğu gözlendi. Uterus'a ait tüm bölümlerin yeşilimsi ve mavimsi bir renk haline geldiği ve uterus'un parlaklık değerinin arttığı belirlendi. Soğuk ortamda dehidrasyon gerçekleştirilmesi ile doku büzüşmesi önemli ölçüde azaldığı belirlendi. Veteriner hekimlik eğitiminde kullanılacak olan genital organ örneklerini hazırlamak için S10B plastinasyon yönteminin kolaylıkla kullanılabileceği sonucuna varıldı.
References
- 1. Thibier M., Wagner HG., 2002. World statistics for artificial insemination in cattle. Livest Prod Sci, 74, 203-212. 2. Oto C., Bakici C., Insal B., Yilmaz B., Ozen D., 2018. Evaluation of the acceptability of fresh dog cadavers in anatomy education. Indian J Anim Res, 808, 1-5. 3. Ramos ML., De Paula TAR., Zerlotini MF., Silva VHD., Carazo LB., De Paula MF., Silva FFR., Santana ML., Silva LC., Ferreira LBC., 2018. A comparison of different de-plastination methodologies for preparing histological sections of material plastinated with Biodur® S10/S3. J Int Soc Plastination, 30, 10-15. 4. Sivagnanam S., Balasundaram K., Selvaraju M., Ravikumar K., Kumaravel A., Paramasivan S., 2014. The polyester resin plastination as a tool for making permanent dry specimens for teaching gynaecology. AJST, 5, 185-186. 5. Riederer BM., 2014. Plastination and its importance in teaching anatomy. Critical points for long-term preservation of human tissue. J Anat, 224, 309-315. 6. Estai M., Bunt S., 2016. Best teaching practices in anatomy education: A critical review. Ann Anat, 208, 151-157. 7. Ottone NE., Cirigliano V., Bianchi HF., Medan CD., Algieri RD., Brum GB., Fuentes R., 2015. New contributions to the development of a plastination technique at room temperature with silicone. Anat Sci Int, 90, 126-135. 8. Ottone NE., Del Sol M., Fuentes R., 2016. Report on a sheet plastination technique using commercial epoxy resin. Int J Morphol, 34, 1039-1043. 9. Ottone NE., Vargas CA., Veuthey C., Del Sol M., Fuentes, R., 2018. Epoxy sheet plastination on a rabbit head–new faster protocol with Biodur® E12/E1. Int J Morphol, 36, 441-446. 10. Steinke H., Rabi S., Saito T., Sawutti A., Miyaki T., Itoh M., Spanel-Borowski K., 2008. Light-weight plastination. Ann Anat, 190, 428-431. 11. Pashaei S., 2010. A brief review on the history, methods and applications of plastination. Int J Morphol, 28, 1075-1079. 12. Suganthy J., Francis DV., 2012. Plastination using standard S10 technique-Our experience in Christian Medical Collage, Vellore. J Anat Soc India, 61, 44-47. 13. Kumro SL., Crocker AV., Powell RL., 2013. Injection plastination: A low-tech, inexpensive method for silicone preservation of small vertebrates. J Int Soc Plastination, 25, 12-17. 14. Gonzalvez M., Ortiz J., Latorre R., 2017. S10 Plastination technique for preservation of parasites: the case of Oestrus ovis larvae. J Int Soc Plastination, 29, 5-10. 15. Latorre RM., Garcia-Sanz MP., Moreno M., Hernandez F., Gil F., Lopez O., Ayala MD., Ramirez G., Vazquez JM., Arencibia A., Henry RW., 2011. How useful is plastination in learning anatomy? JVME, 34, 172-176. 16. Chapman SJ., Hakeem AR., Marangoni G., Prasad KR., 2013. Anatomy in medical education: Perceptions of undergraduate medical students. Ann Anat, 195, 409-414. 17. Sivagnanam S., Balasundaram K., Selvaraju M., Ravikumar K., Kumaravel A., Paramasivan S., 2014. The epoxy resin plastination of reproductive organs of animals. AJST, 5, 181-182. 18. Brown MA., Reed RB., Henry RW., 2002. Effects of dehydration mediums and temperature on total dehydration time and tissue shrinkage. J Int Soc Plastination, 17, 28-33. 19. Ekim O., İnsal B., Bakıcı C., Hazıroğlu RM., Akgün RO., 2014a. Yılanlarda Soğuk Ortam Tekniği ile Tüm Vücut Silikon Plastinasyonu. Dicle Üniv Vet Fak Derg, 1, 1-14. 20. Ekim O., Tunalı S., Hazıroğlu RM., Ayvalı M., 2014. Evcil memeli hayvanlarda böbreklerin soğuk ortam tekniği ile silikon plastinasyonu. Vet Hekim Der Derg, 85, 1-11.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Araştırma Makaleleri |
| Authors | |
| Publication Date | October 25, 2019 |
| Published in Issue | Year 2019 Volume: 14 Issue: 2 |