Serum Amiloid-A'nın Sıçanlarda Formaldehit Kaynaklı Kupffer Hücre Apoptozundaki Rolü ve Astaksantin'in Bu Süreçteki Olası Koruyucu Etkileri

Year 2020, Volume: 15 Issue: 1, 22 - 30, 30.04.2020

Aykut Ulucan , Hayati Yüksel , Emre Şahin , Seda Yakut

https://doi.org/10.17094/ataunivbd.675315

Abstract

Bu çalışmanın amacı Kupffer hücrelerinde formaldehit kaynaklı apoptozda Serum amiloid-A ile ilgili değişiklikleri araştırmak ve Astaksantin'in apoptoza karşı koruyucu bir etkisi olup olmadığını belirlemektir. Bu deneyde 32 sıçan 4 gruba ayrıldı (n = 8). Birinci gruba kontrol grubu adı verildi ve serum fizyolojik intraperitoneal olarak bu gruba enjekte edildi ve içme suyu oral yolla verildi. CH20 grubunda, sıçanlara günde 10 mg/kg dozda intraperitonal yoldan formaldehit enjekte edildi. CH2O+ATX16 ve CH2O+ATX32'deki gruplarındaki sıçanlara günde 10 mg/kg intraperitoneal dozda formaldehit enjekte edildi ve sırasıyla 16 mg/kg ve 32 mg/kg Astaksantin oral yolla verildi. Formaldehit uygulaması CH2O grubunda en yüksek seviyede ve istatistiksel olarakta anlamlı Serum amiloid-A boyama yoğunluğuna (P<0.0125) ve apoptotik indekse (P<0.05) neden olmuştur. Her iki dozdaki Astaksantin uygulaması Kupffer hücrelerinde apoptozu azalttı, ancak deney grupları arasında serum Serum amiloid-A düzeylerinde anlamlı bir fark yoktu (P>0.05). Sonuç olarak, oral yolla Astaksantin uygulamasının formaldehit maruziyetine bağlı olarak artan Serum Amiloid A'yı azalttığı ve muhtemelen bu şekilde Kupffer hücrelerinin formaldehit kaynaklı apoptoza karşı başarıyla koruduğu gösterilmiştir. Konu daha kapsamlı bir şekilde incelenmelidir

Keywords

: Apoptoz, Astaksantin, Formaldehit, Kupffer Hücresi, Serum Amiloid-A

Supporting Institution

Bingöl Üniversitesi

Project Number

BAP-VF.2017.00.001

Thanks

Bingöl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından kabul edilen ÇOK DİSİPLİNLİ ARAŞTIRMA PROJELERİ türündeki BAP-VF.2017.00.001 nolu ve "Deneysel Formaldehid Toksikasyonunda Astaksantinin Rat Testis Dokusu Üzerindeki Koruyucu Etkilerinin İncelenmesi" başlıklı bu proje, "Bingöl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir". Bu projeye katkı sunan Bingöl Üniversitesi Rektörlüğü ve Bilimsel Araştırma Projeleri Koordinasyon Birimi başta olmak üzere tüm proje ekibi çalışanlarına ve araştırmacılarına teşekkürlerimi sunarım.

The Role of Serum Amyloid-A in Formaldehyde-Induced Kupffer Cell Apoptosis in Rats and Possible Protective Effects of Astaxanthin in This Process

Abstract

The aim of this study is to investigate the alterations related to Serum amyloid-A in formaldehyde-induced apoptosis in Kupffer cells and to determine whether Astaxanthin has a protective effect against apoptosis. In this experiment, 32 rats were divided into 4 groups (n=8). The first group was named as control group, physiological saline was injected intraperitoneally to this group, and drinking water was given orally. In CH2O group, rats were injected with formaldehyde at a dose of 10 mg/kg daily intraperitoneally. The rats in CH2O+ATX16 and CH2O+ATX32 were injected with formaldehyde daily at a dose of 10 mg/kg intraperitoneally, and respectively 16 mg/kg and 32 mg/kg Astaxanthin were administered orally. Formaldehyde administration was caused by the highest and statistically significant Serum amyloid-A staining intensity (P<0.0125) and apoptotic index (P<0.05) in the CH2O group. Both doses of Astaxanthin administration reduced apoptosis in Kupffer cells but there were no significant differences in serum Serum amyloid-A levels between experimental groups (P>0.05). As a result, oral administration of Astaxanthin has been shown to reduce Serum Amyloid A, which increases due to exposure to formaldehyde, and possibly in this way, Kupffer cells successfully protect against formaldehyde-induced apoptosis. The subject should be examined more comprehensively

Keywords

Serum Amyloid-A, Apoptosis, Astaxanthin, Formaldehyde, Kupffer Cell, Serum Amyloid-A

Project Number

BAP-VF.2017.00.001

References

• 1. Hovda KE., McMartin K., Jacobsen D., 2017. Methanol and Formaldehyde. Critical Care Toxicology: Diagnosis and Management of the Critically Poisoned Patient, 1: 1769-86. Springer Publishing. 2. Zendehdel R., Jouni FJ., Hajipour B., Panjali Z., Kheiri H., Vahabi M., 2017. DNA damage in workers exposed to formaldehyde at concentrations below occupational exposure limits. Toxicol Environ Chem, 99, 1409-1417. 3. Li L., Hua L., He Y., Bao Y., 2017. Differential effects of formaldehyde exposure on airway inflammation and bronchial hyperresponsiveness in BALB/c and C57BL/6 mice. PloS one, 12, e0179231. 4. Songur A., Sarsilmaz M., Ozen O., Sahin S., Koken R., Zararsiz I., Ilhan N., 2008. The effects of inhaled formaldehyde on oxidant and antioxidant systems of rat cerebellum during the postnatal development process. Toxicol Mech Method, 18, 569-574. 5. Szende B., Tyihak E., 2010. Effect of formaldehyde on cell proliferation and death. Cell Biol Int, 34, 1273-1282. 6. Bakar E., Ulucam E., Cerkezkayabekir A., 2015. Investigation of the protective effects of proanthocyanidin and vitamin E against the toxic effect caused by formaldehyde on the liver tissue. Environ Toxicol, 30, 1406-1415. 7. Peng J., Yuan J-P., Wang J-H., 2012. Effect of diets supplemented with different sources of astaxanthin on the gonad of the sea urchin Anthocidaris crassispina. Nutrients, 4, 922-934. 8. Jyonouchi H., Sun S., Iijima K., Gross MD., 2000. Antitumor activity of astaxanthin and its mode of action. Nutr Cancer,36, 59-65. 9. Kessler SM., Hoppstadter J., Hosseini K., Laggai S., Haybaeck J., Kiemer AK., 2019. Lack of Kupffer cell depletion in diethylnitrosamine-induced hepatic inflammation. J Hepatol, 70, 813-815. 10. Bode JG., Albrecht U., Haussinger D., Heinrich PC., Schaper F., 2012. Hepatic acute phase proteins–regulation by IL-6-and IL-1-type cytokines involving STAT3 and its crosstalk with NF-κB-dependent signaling. Eur J Cell Biol, 91, 496-505. 11. Lin K-H., Lin K-C., Lu W-J., Thomas P-A., Jayakumar T., Sheu J-R., 2016. Astaxanthin, a carotenoid, stimulates immune responses by enhancing IFN-γ and IL-2 secretion in primary cultured lymphocytes in vitro and ex vivo. Int J Mol Sci, 17, 44. 12. Suvarna KS., Layton C., Bancroft JD., 2018. Bancroft's Theory and Practice of Histological Techniques E-Book: Elsevier Health Sciences. 13. Vickers J., 2019. Immunohistochemistry techniques applicable for use with human brain tissue. In Using CNS Autopsy Tissue in Psychiatric Research: A Practical Guide, pp. 117-36: CRC Press. 14. Aydin S., Ogeturk M., Kuloglu T., Kavakli A., Aydin S., 2015. Effect of carnosine supplementation on apoptosis and irisin, total oxidant and antioxidants levels in the serum, liver and lung tissues in rats exposed to formaldehyde inhalation. Peptides, 64, 14-23. 15. Mengshol JA., Golden-Mason L., Arikawa T., Smith M., Niki T., McWilliams R., Randall JA., McMahan R., Zimmerman MA., Rangachari M., Dobrinskikh E., Busson P., Polyak SP., Hirashima M., Rosen HR., 2010. A crucial role for kupffer cell-derived galectin-9 in regulation of t cell immunity in hepatitis c infection. PloS one, 5, 1-12. 16. Ekuni D., Tomofuji T., Sanbe T., Irie K., Azuma T., Maruyama T., Tamaki N., Murakami J., Kokeguchi S., Yamamoto T., 2009. Vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress. Arch Oral Biol, 54, 495-502. 17. Aydemir S., Akgun SG., Beceren A., Yuksel M., Kumas M., Erdogan N., Sardas S., Omurtag GZ., 2017. Melatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats. Int J Clin Exp Med, 10, 6250-6261. 18. Wei C., Wen H., Yuan L., McHale CM., Li H., Wang K., Yuan J., Yang X., Zhang L., 2017. Formaldehyde induces toxicity in mouse bone marrow and hematopoietic stem/progenitor cells and enhances benzene-induced adverse effects. Arch Toxicol, 91, 921-933. 19. Reilly SM., Goel R., Trushin N., Elias RJ., Foulds J., Muscat J., Liao J., Richie Jr JP., 2017. Brand variation in oxidant production in mainstream cigarette smoke: Carbonyls and free radicals. Food Chem Toxıcol, 106, 147-154. 20. Vina J., 2018. Free Radical Theory Of Frailty: Molecular Mechanisms of Frailty Resulting From Oxidatıve Stress. Innov Aging, 2, 219. 21. Cikmaz S., Kutoglu T., Kanter M., Mesut R., 2010. Effect of formaldehyde inhalation on rat livers: a light and electron microscopic study. Toxicol and Health, 26, 113-119. 22. Özen OA., Kus I., Bakirdere S., Sarsilmaz M., Yaman M., 2011. Effects of formaldehyde inhalation on zinc, copper and iron concentrations in liver and kidney of male rats. Biol Trace Elem Res, 140, 177-185. 23. Treesh S., Eljaafari H., Darmun E., Abu-Aisha A., Alwaer F., Eltubuly R., Elghedamsi M., Aburawi S., 2014. Histologıcal study on the effect of formaldehyde on mice liver and kidney and possible protective role of selenium. J Cell Tissue Res, 14, 4201-4209. 24. Cheng J., Zhang L., Tang Y., Li Z., 2016. The toxicity of continuous long-term low-dose formaldehyde inhalation in mice. Immunopharm Immunot, 38, 495-501. 25. Nasiri E., Naserirad S., Pasdaran Lashgari A.,Gazor R., Mohammadghasemi F., Atrkar Roushan Z., 2016. Hepatoprotective effect of Acantholimon bracteatum (Girard) Boiss. on formaldehyde-induced liver injury in adult male mice. RJP, 3, 55-61. 26. Nguyen-Lefebvre AT., Horuzsko A., 2015. Kupffer cell metabolism and function. J Enzymol Metab, 1, 101. 27. Liu H., Li Q., Wang Y., Hong H., Chen M., Wang Y., Hong F., Yang S., 2017. Elevated nitric oxide levels associated with hepatic cell apoptosis during liver injury. Hepatol Res, 47, 178-185. 28. Iorga A., Dara L., Kaplowitz N., 2017. Drug-induced liver injury: cascade of events leading to cell death, apoptosis or necrosis. Int J Mol Sci, 18, 1018. 29. Canbay A., Feldstein AE., Higuchi H., Werneburg N., Grambihler A., Bronk SF., Gores GJ., 2003. Kupffer cell engulfment of apoptotic bodies stimulates death ligand and cytokine expression. Hepatology, 38, 1188-1198. 30. Dhankhar J., Kadian SS., Sharma A., 2012. Astaxanthin: A potential carotenoid. IJPSR, 3, 1246. 31. Jang H., Ji S., Kim Y., Lee H., Shin J., Cheong H., Kim J., Park I., Kong H., Park C., 2010. Antioxidative effects of astaxanthin against nitric oxide‐induced oxidative stress on cell viability and gene expression in bovine oviduct epithelial cell and the developmental competence of bovine IVM/IVF embryos. Reprod Domest Anim, 45, 967-974. 32. Cameron RG., Feuer G., 2012. Apoptosis and its modulation by drugs: Springer Science & Business Media. 33. Kuret T., Lakota K., Mali P., Cucnik S., Praprotnik S., Tomšič M., Sodin-Semrl S., 2018. Naturally occurring antibodies against serum amyloid A reduce IL-6 release from peripheral blood mononuclear cells. PloS one, 13, e0195346.