Abstract
Bu çalışmanın amacı restoratif dental materyallerin, sıçan böbrek ve karaciğer dokuları üzerindeki olası etkilerinin incelenmesidir. 32 adet, yetişkin, dişi Wistar albino cinsi ratlar Kontrol grubu, Dental amalgam grubu, Rezin kompozit grubu, Cam iyonomer grubu olmak üzere 4 gruba ayrılmış (n=8) ve ense kısımlarına yapılan kesilerle dental materyaller yerleştirilip 8 hafta beklenmiştir. Ötenaziden sonra yapılan nekropsi ile karaciğer ve böbrek dokuları alınmıştır. Kullanılan boyama yöntemleri, NF-κB p65 ve Hematoksilen&Eozin boyamadır. Biyokimyasal analiz olarak ise glutatyon, süper oksit dismutaz, lipit peroksidasyon ve katalaz seviyeleri ölçülmüştür. İmmün-pozitif hücreler, kontrol grubunda deney gruplarına göre daha az yoğunluk göstermiştir. Bununla birlikte, tüm deney gruplarında renal ve hepatik dejenerasyonlar görülmüştür. Tüm biyokimyasal analiz sonuçları, kontrol grubu hariç deney gruplarında oksidatif stres oluştuğunu göstermektedir. Çalışmamızda elde ettiğimiz sonuçlar, deney gruplarında kullanılan tüm restoratif dental materyallerin böbrek ve karaciğer dokusunda sitotoksik etki yarattığını göstermektedir. Çalışmamızda kullanılan restoratif dental materyaller, tüm deney gruplarında böbrek ve karaciğer dokularında oksidatif stres oluşmasıyla ilişkilendirilebilir
Supporting Institution
TÜBİTAK
Project Number
114S881
References
- 1. Yang L., Zhang Y., Wang F., Luo Z., Guo S., Strahle U., 2020. Toxicity of mercury: Molecular evidence. Chemosphere, 245, 125586. 2. Bayne SC., Ferracane JL., Marshall GW., Marshall SJ., van Noort R., 2019. The evolution of dental materials over the past century: Silver and gold to tooth color and beyond. J Dent Res, 98, 257-265. 3. Celik N., Binnetoglu D., Ozakar Ilday N., Hacimuftuoglu A., Seven N., 2019. The cytotoxic and oxidative effects of restorative materials in cultured human gingival fibroblasts. Drug Chem Toxicol, 31, 1-6. 4. Bandarra S., Mascarenhas P., Luis AR., Catrau M., Bekman E., Ribeiro AC., Felix S., Caldeira J., Barahona I., 2020. In vitro and in silico evaluations of resin-based dental restorative material toxicity. Clin Oral Investig, 24, 2691-2700. 5. Yi J., Weir MD., Melo MAS., Li T., Lynch CD., Oates TW., Dai Q., Zhao Z., Xu HHK., 2019. Novel rechargeable nano-CaF2 orthodontic cement with high levels of long-term fluoride release. J Dent, 90, 103214. 6. Lingappan K., 2018. NF-kappaB in oxidative stress. Curr Opin Toxicol, 7, 81-86. 7. Opdam NJM., Collares K., Hickel R., Bayne SC., Loomans BA., Cenci MS., Lynch CD., Correa MB., Demarco F., Schwendicke F., Wilson NHF., 2018. Clinical studies in restorative dentistry: New directions and new demands. Dent Mater, 34, 1-12. 8. Cosgun A., Bolgul B., Duran N., 2019. In vitro investigation of antimicrobial effects, nanohardness, and cytotoxicity of different glass ionomer restorative materials in dentistry. Niger J Clin Pract, 22, 422-431. 9. Samir AM., Aref WM., 2011. Impact of occupational exposure to elemental mercury on some antioxidative enzymes among dental staff. Toxicol Ind Health, 27, 779-786. 10. Can S., Cigsar G., Gur Ozabacigil F., Aksak Karamese S., Selli J., Bacak G., Gedikli S., Sahin GZ., Yigit S., Can İ., Gul M., 2015. Hepatoprotective effect of 17beta-estradiol as antioxidant modulators against stress damage. Hepat Mon, 5, e22633. 11. Unal D., Aksak S., Halici Z., Sengul O., Polat B., Unal B., Halici M., 2011. Effects of diabetes mellitus on the rat liver during the postmenopausal period. J Mol Histol, 42, 273-87. 12. Brownawell AM., Berent S., Brent RL., Bruckner JV., Doull J., Gershwin EM., Hood RD., Matanoski GM., Rubin R., Weiss B., Karol MH., 2005. The potential adverse health effects of dental amalgam. Toxicol Rev, 24, 1-10. 13. Horsted-Bindslev P., 2004. Amalgam toxicity-environmental and occupational hazards. J Dent, 32, 359-365. 14. Schwengberg S., Bohlen H., Kleinsasser N., Kehe K., Seiss M., Walther UI., Hickel R., Reichl FX., 2005. In vitro embryotoxicity assessment with dental restorative materials. J Dent, 33, 49-55. 15. Urcan E., Scherthan H., Styllou M., Haertel U., Hickel R., Reichl FX., 2010. Induction of DNA double-strand breaks in primary gingival fibroblasts by exposure to dental resin composites. Biomaterials, 31, 2010-2014. 16. Nakamura K., Itoh K., Sugimoto T., Fushiki S., 2007. Prenatal exposure to bisphenol A affects adult murine neocortical structure. Neurosci Lett, 420, 100-105. 17. Ramireddy S., Mahendra J., Rajaram V., Ari G., Kanakamedala AK., Krishnakumar D., 2018. Treatment of gingival recession by coronally advanced flap in conjunction with platelet-rich fibrin or resin-modified glass-ionomer restoration: A clinical study. J Indian Soc Periodontol, 22, 45-49. 18. Al-Gerny YA., Ghorab SM., Soliman TA., 2019. Bond strength and elemental analysis of oxidized dentin bonded to resin modified glass ionomer based restorative material. J Clin Exp Dent, 11, e250-e256. 19. Sasanaluckit P., Albustany KR., Doherty PJ., Williams DF., 1993. Biocompatibility of glass ionomer cements. Biomaterials, 14, 906-916. 20. Bradley PP., Priebat DA., Christensen RD., Rothstein G., 1982. Measurement of cutaneous inflammation: Estimation of neutrophil content with an enzyme marker. J Invest Dermatol, 78, 206-209. 21. Circu ML., Aw TY., 2012. Glutathione and modulation of cell apoptosis. Biochim Biophys Acta, 1823, 1767-1777. 22. Jagadeesan G., Sankarsami Pillai S., 2007. Hepatoprotective effects of taurine against mercury induced toxicity in rats. J Environ Biol, 28, 753-756. 23. Chang HH., Guo MK., Kasten FH., Chang MC., Huang GF., Wang YL., Wang RS., Jeng JH., 2005. Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials, 26, 745-753. 24. Mantellini MG., Botero TM., Yaman P., Dennison JB., Hanks CT., Nor JE., 2003. Adhesive resin induces apoptosis and cell-cycle arrest of pulp cells. J Dent Res, 82, 592-596. 25. Sharma MK., Kumar M., Kumar A., 2005. Protection against mercury-induced renal damage in Swiss albino mice by Ocimum sanctum. Environ Toxicol Pharmacol, 19, 161-167
Abstract
The purpose of this study was to examine the potential effects of restorative dental materials on the kidneys and liver tissues of rats. The histological, immunohistochemical and biochemical analyzes were conducted using 32 adult female Wistar albino rats classified into 4 groups (n=8): Control group, Dental amalgam group, Resin composite group, and Glass-ionomer group. The dental materials mentioned were put on the backs of the rat heads and kept there for 8 weeks. After humanely euthanizing rats, kidney and liver tissue was removed by necropsy, and Hematoxylin&Eosin and NF-κB p65 staining methods were used. In addition, glutathione, superoxide dismutase, lipid peroxidation and catalase levels have been identified. Immune-positive cells were higher in all sample groups than in the control group. Renal and hepatic degeneration has also been found in dental filling content groups. All biochemical findings revealed oxidative stress in all groups with the exception for control group. The three dental filling products have significant cytotoxic effects on the kidney and liver tissues, and may have been linked with oxidative stress in the tissues of the kidney and liver.
Project Number
114S881
References
- 1. Yang L., Zhang Y., Wang F., Luo Z., Guo S., Strahle U., 2020. Toxicity of mercury: Molecular evidence. Chemosphere, 245, 125586. 2. Bayne SC., Ferracane JL., Marshall GW., Marshall SJ., van Noort R., 2019. The evolution of dental materials over the past century: Silver and gold to tooth color and beyond. J Dent Res, 98, 257-265. 3. Celik N., Binnetoglu D., Ozakar Ilday N., Hacimuftuoglu A., Seven N., 2019. The cytotoxic and oxidative effects of restorative materials in cultured human gingival fibroblasts. Drug Chem Toxicol, 31, 1-6. 4. Bandarra S., Mascarenhas P., Luis AR., Catrau M., Bekman E., Ribeiro AC., Felix S., Caldeira J., Barahona I., 2020. In vitro and in silico evaluations of resin-based dental restorative material toxicity. Clin Oral Investig, 24, 2691-2700. 5. Yi J., Weir MD., Melo MAS., Li T., Lynch CD., Oates TW., Dai Q., Zhao Z., Xu HHK., 2019. Novel rechargeable nano-CaF2 orthodontic cement with high levels of long-term fluoride release. J Dent, 90, 103214. 6. Lingappan K., 2018. NF-kappaB in oxidative stress. Curr Opin Toxicol, 7, 81-86. 7. Opdam NJM., Collares K., Hickel R., Bayne SC., Loomans BA., Cenci MS., Lynch CD., Correa MB., Demarco F., Schwendicke F., Wilson NHF., 2018. Clinical studies in restorative dentistry: New directions and new demands. Dent Mater, 34, 1-12. 8. Cosgun A., Bolgul B., Duran N., 2019. In vitro investigation of antimicrobial effects, nanohardness, and cytotoxicity of different glass ionomer restorative materials in dentistry. Niger J Clin Pract, 22, 422-431. 9. Samir AM., Aref WM., 2011. Impact of occupational exposure to elemental mercury on some antioxidative enzymes among dental staff. Toxicol Ind Health, 27, 779-786. 10. Can S., Cigsar G., Gur Ozabacigil F., Aksak Karamese S., Selli J., Bacak G., Gedikli S., Sahin GZ., Yigit S., Can İ., Gul M., 2015. Hepatoprotective effect of 17beta-estradiol as antioxidant modulators against stress damage. Hepat Mon, 5, e22633. 11. Unal D., Aksak S., Halici Z., Sengul O., Polat B., Unal B., Halici M., 2011. Effects of diabetes mellitus on the rat liver during the postmenopausal period. J Mol Histol, 42, 273-87. 12. Brownawell AM., Berent S., Brent RL., Bruckner JV., Doull J., Gershwin EM., Hood RD., Matanoski GM., Rubin R., Weiss B., Karol MH., 2005. The potential adverse health effects of dental amalgam. Toxicol Rev, 24, 1-10. 13. Horsted-Bindslev P., 2004. Amalgam toxicity-environmental and occupational hazards. J Dent, 32, 359-365. 14. Schwengberg S., Bohlen H., Kleinsasser N., Kehe K., Seiss M., Walther UI., Hickel R., Reichl FX., 2005. In vitro embryotoxicity assessment with dental restorative materials. J Dent, 33, 49-55. 15. Urcan E., Scherthan H., Styllou M., Haertel U., Hickel R., Reichl FX., 2010. Induction of DNA double-strand breaks in primary gingival fibroblasts by exposure to dental resin composites. Biomaterials, 31, 2010-2014. 16. Nakamura K., Itoh K., Sugimoto T., Fushiki S., 2007. Prenatal exposure to bisphenol A affects adult murine neocortical structure. Neurosci Lett, 420, 100-105. 17. Ramireddy S., Mahendra J., Rajaram V., Ari G., Kanakamedala AK., Krishnakumar D., 2018. Treatment of gingival recession by coronally advanced flap in conjunction with platelet-rich fibrin or resin-modified glass-ionomer restoration: A clinical study. J Indian Soc Periodontol, 22, 45-49. 18. Al-Gerny YA., Ghorab SM., Soliman TA., 2019. Bond strength and elemental analysis of oxidized dentin bonded to resin modified glass ionomer based restorative material. J Clin Exp Dent, 11, e250-e256. 19. Sasanaluckit P., Albustany KR., Doherty PJ., Williams DF., 1993. Biocompatibility of glass ionomer cements. Biomaterials, 14, 906-916. 20. Bradley PP., Priebat DA., Christensen RD., Rothstein G., 1982. Measurement of cutaneous inflammation: Estimation of neutrophil content with an enzyme marker. J Invest Dermatol, 78, 206-209. 21. Circu ML., Aw TY., 2012. Glutathione and modulation of cell apoptosis. Biochim Biophys Acta, 1823, 1767-1777. 22. Jagadeesan G., Sankarsami Pillai S., 2007. Hepatoprotective effects of taurine against mercury induced toxicity in rats. J Environ Biol, 28, 753-756. 23. Chang HH., Guo MK., Kasten FH., Chang MC., Huang GF., Wang YL., Wang RS., Jeng JH., 2005. Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials, 26, 745-753. 24. Mantellini MG., Botero TM., Yaman P., Dennison JB., Hanks CT., Nor JE., 2003. Adhesive resin induces apoptosis and cell-cycle arrest of pulp cells. J Dent Res, 82, 592-596. 25. Sharma MK., Kumar M., Kumar A., 2005. Protection against mercury-induced renal damage in Swiss albino mice by Ocimum sanctum. Environ Toxicol Pharmacol, 19, 161-167
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Araştırma Makaleleri |
| Authors | |
| Project Number | 114S881 |
| Publication Date | December 31, 2020 |
| Published in Issue | Year 2020 Volume: 15 Issue: 3 |