Research Article
Year 2020,
Volume: 15 Issue: 3, 294 - 300, 31.12.2020
Abstract
The antioxidant and protective effect of resveratrol against irradiation induced oxidative damage were researched. Twenty-one rats were seperated into 3 groups; First group was the control group. Second group was the irradiation group; received physiological saline intraperitoneally (i.p.) for three days, then 9 Gray (Gy) irradiation was applied. Third group was irradiation+Resveratrol group; received 10 mg/kg resveratrol i.p. for three days and then the same dose of radiation was applied. As a result of irradiation, a significant increase was found in the level of malondialdehyde (MDA) in the liver, kidney and brain tissues of rats (P˂0.001). Glutathione peroxidase (GSH-Px) activity in all the tissues and superoxide dismutase (SOD) activity, Glutathione (GSH) levels in liver decreased (P˂0.01), GSH levels in kidney, spleen and ovarium tissues significantly increased (P˂0.05). The giving of resveratrol significantly reduced the MDA levels increasing in liver, kidney, and brain as a result of irradiation (P˂0.001) and the liver MDA levels reached to the control levels. By giving resveratrol, it was determined that all the antioxidants studied reduced significantly compared to the control group and/or the irradiation group (P˂0.05). According to the data obtained as a result; It was concluded that resveratrol shows a corrective effect by changing the oxidant/antioxidant balance against oxidative stress caused by irradiation, therefore resveratrol is a powerful antioxidant.
Keywords
References
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Identification of mammalian proteins cross-linked to DNA by ionizing gradiation. J Biol Chem, 280, 33826-33838. 7. Sener TE., Tavukcu HH., Atasoy BM., Cevik O., Kaya OT., Cetinel S., Dagli Degerli A., Tinay I., Simsek F., Akbal C., Buttice S., Sener G., 2018. Resveratrol treatment may preserve the erectile function after radiotherapy by restoring antioxidant defence mechanisms, SIRT1 and NOS protein expressions. Int J Impot Res, 30, 179-188. 8. Bradamante S., Barenghi L., Villa A., 2004. Cardiovascular protective effects of resveratrol. Cardiovasc Drug Rev, 22, 169-188. 9. Velioğlu-Oğünç AI., Sehirli O., Toklu HZ., Ozyurt H., Mayadağli A., Ekşioğlu-Demiralp E., Erzik C., Cetinel S., Yeğen BC., Sener G., 2009. Resveratrol protects against irradiation-induced hepatic and ileal damage via its anti-oxidative activity. Free Radic Res, 43, 1060-1071. 10. Khattab HAH., Abdallah IZA., Yousef FM., Huwait EA., 2017. Efficiency of borage seeds oil against gamma irradiation-induced hepatotoxicity in male rats: possible antioxidant activity. Afr J Tradit Complement Altern Med, 14, 169-179. 11. Radwan RR., Mohamed HA., 2018. Nigella sativa oil modulates the therapeutic efficacy of mesenchymal stem cells against liver injury in irradiated rats. J Photochem Photobiol B, 178, 447-456. 12. Benkovica V., Knezevica AH., Dikica D., Lisicic D., Orsolic N., Basic I., kosalec I., Kopjar N., 2008. Radioprotective effects of propolis and quercetin in gamma-irradiated mice evaluated by the alkaline comet assay. Phytomedicine, 15, 851-858. 13. Placer ZA., Cushman LL., Johnson BC., 1966. Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical systems. Anal Biochem, 16, 359-364. 14. Sedlak J., Lindsay RH., 1968. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem, 25, 192-205. 15. Lawrence RA., Burk RF., 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun, 71, 952-958. 16. Sun Y., Oberley WL., Li Y., 1988. A simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500. 17. Lowry OH., Rosebrough NJ., Farr AL., Randall RJ., 1951. Protein measurement with folin phenol reagent. J Biol Chem, 193, 265-275. 18. Cuzzocrea S., Reiter RJ., 2001. Pharmacological action of melatonin in shock, inflammation and ischemia/reperfusion injury. Europ J Pharmacol, 426, 1-10. 19. Li J., Feng L., Xing Y., Wang Y., Du L., Xu C., Cao J., Wang Q., Fan S., Liu Q., Fan F., 2014. Radioprotective and antioxidant effect of resveratrol in hippocampus by activating Sirt1. Int J Mol Sci, 15, 5928-5939. 20. de Freitas RB., Boligon AA., Rovani BT., Piana M., de Brum TF., da Silva Jesus R., Rother FC., Alves NM., Teixeira da Rocha JB., Athayde ML., Barrio JP., de Andrade ER., de Freitas Bauerman L., 2013. Effect of black grape juice against heart damage from acute gamma TBI in rats. Molecules, 18, 12154-12167. 21. Xie Y., Zhao QY., Li HY., Zhou X., Liu Y., Zhang H., 2014. Curcumin ameliorates cognitive deficits heavy ion irradiation-induced learning and memory deficits through enhancing of Nrf2 antioxidant signaling pathways. Pharmacol Biochem Behav, 126, 181-186. 22. Karami M., Asri-Rezaei S., Dormanesh B., Nazarizadeh A., 2018. Comparative study of radioprotective effects of selenium nanoparticles and sodium selenite in irradiation-induced nephropathy of mice model. Int J Radiat Biol, 94, 17-27. 23. Sener G., Kabasakal L., Atasoy BM., Erzik C., Velioğlu-Oğünç A., Cetinel S., Gedik N., Yeğen BC., 2006. Ginkgo biloba extract protects against ionizing radiation-induced oxidative organ damage in rats. Pharmacol Res, 53, 241-252. 24. Ekici K., Temelli O., Parlakpinar H., Samdanci E., Polat A., Beytur A., Tanbek K., Ekici C., Dursun IH., 2016. Beneficial effects of aminoguanidine on radiotherapy-induced kidney and testis injury. Andrologia, 48, 683-692. 25. Cheng C., Yi J., Wang R., Cheng L., Wang Z., Lu W., 2018. Protection of Spleen Tissue of γ-ray Irradiated Mice against Immunosuppressive and Oxidative Effects of Radiation by Adenosine 5'-Monophosphate. Int J Mol Sci, 19, E1273. 26. Said RSI., Nada AS., El-Demerdash E., 2012. Sodium selenite improves folliculogenesis in radiation-induced ovarian failure: a mechanistic approach. PLoS One, 7, e50928. 27. Simsek Y., Gurocak S., Turkoz Y., Akpolat N., Celik O., Ozer A., Yılmaz E., Turhan U., Ozyalın F., 2012. Ameliorative effects of resveratrol on acute ovarian toxicity induced by total body irradiation in young adult rats. J Pediatr Adolesc Gynecol, 25, 262-266. 28. Hermes-Lima M., Storey JM., Storey KB., 1998. Antioxidant defenses and metabolic depression. The hypothesis of preparation for oxidative stress in land snails. Comp Biochem Physiol B-Biochem Mol Biol, 120, 437-448. 29. Black HS., 2004. Reassessment of a free radical theory of cancer with emphasis on ultraviolet carcinogenesis. Integr Cancer Ther, 3, 279-293. 30. Kucukoglu K., Nadaroglu H., 2014. Assessment of the antioxidant and antiradicalic capacities in vitro of different phenolic derivatives. Free Rad Antiox, 4, 24-31. 31. Simsek GI., Gurocak S., Karadag N., Karabulut AB., Demirtas E., Karataş E., Pepele E., 2012. Protective effects of resveratrol on salivary gland damage induced by total body irradiation in rats. Laryngoscope, 122, 2743-2748. 32. Xu L., Yang X., Cai J., Ma J., Cheng H., Zhao K., Yang L., Cao Y., Qin Q., Zhang C., Zhang Q., Sun X., 2013. Resveratrol attenuates radiation-induced salivary gland dysfunction in mice. Laryngoscope,123, E23-29. 33. Saada HN., Said UZ., Meky NH., Abd EI., Azime AS., 2009. Grape seed extract Vitis vinifera protects against radiation-induced oxidative damage and metabolic disorders in rats. Phytother Res, 23, 434-438. 34. Cho BO., Che DN., Shin JY., Kang HJ., Jang SI., 2018. Ameliorative effects of fruit stem extract from Muscat Bailey A against chronic UV-induced skin damage in BALB/c mice. Biomed Pharmacother, 97,1680-1688.
Year 2020,
Volume: 15 Issue: 3, 294 - 300, 31.12.2020
Abstract
Radyasyonun yarattığı oksidatif hasar üzerine resveratrolün antioksidan ve koruyucu etkisi araştırıldı. Yirmi bir rat 3 gruba ayrıldı; Birinci grup, Kontrol grubu. İkinci grup, Radyasyon grubu; Üç gün fizyolojik tuz solüsyonu intraperitoneal (i.p.) olarak verildi ve sonra 9 Gray (Gy) radyasyon uygulandı. Üçüncü grup, Radyasyon + Resveratrol grubu; Üç gün 10 mg/kg olacak şekilde resveratrol i.p. verildi ve sonra aynı dozda radyasyon uygulandı. Radyasyon sonucu ratların karaciğer, böbrek ve beyin dokularında malondialdehit (MDA) seviyesinde önemli artış saptandı (P˂0.001). Çalışılan tüm dokularda glutatyon peroksidaz (GSH-Px) aktivitesinde (P˂0.01), yine karaciğer süperoksit dismutaz (SOD) aktivitesi ve glutatyon (GSH) seviyesinde azalış (P˂0.01), böbrek, dalak ve ovarium dokularında GSH düzeyinde ise önemli artış tespit edildi (P˂0.05). Resveratrol verilmesi radyasyon sonucu karaciğer, böbrek ve beyinde artan MDA düzeylerini önemli azalttı (P˂0.001) ve karaciğer MDA düzeyleri kontrol düzeylerine ulaştı. Resveratrol verilmesiyle çalışılan tüm antioksidanların kontrol ve/veya radyasyon uygulanan gruba göre önemli azalma gösterdiği tespit edildi (P˂0.05). Sonuç olarak elde edilen verilere göre; resveratrolün radyasyona bağlı oluşan oksidatif strese karşı oksidan/antioksidan dengeyi değiştirerek düzeltici bir etki gösterdiği, bu nedenle resveratrolün güçlü bir antioksidan olduğu kanaatine varılmıştır.
Keywords
References
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Identification of mammalian proteins cross-linked to DNA by ionizing gradiation. J Biol Chem, 280, 33826-33838. 7. Sener TE., Tavukcu HH., Atasoy BM., Cevik O., Kaya OT., Cetinel S., Dagli Degerli A., Tinay I., Simsek F., Akbal C., Buttice S., Sener G., 2018. Resveratrol treatment may preserve the erectile function after radiotherapy by restoring antioxidant defence mechanisms, SIRT1 and NOS protein expressions. Int J Impot Res, 30, 179-188. 8. Bradamante S., Barenghi L., Villa A., 2004. Cardiovascular protective effects of resveratrol. Cardiovasc Drug Rev, 22, 169-188. 9. Velioğlu-Oğünç AI., Sehirli O., Toklu HZ., Ozyurt H., Mayadağli A., Ekşioğlu-Demiralp E., Erzik C., Cetinel S., Yeğen BC., Sener G., 2009. Resveratrol protects against irradiation-induced hepatic and ileal damage via its anti-oxidative activity. Free Radic Res, 43, 1060-1071. 10. Khattab HAH., Abdallah IZA., Yousef FM., Huwait EA., 2017. Efficiency of borage seeds oil against gamma irradiation-induced hepatotoxicity in male rats: possible antioxidant activity. Afr J Tradit Complement Altern Med, 14, 169-179. 11. Radwan RR., Mohamed HA., 2018. Nigella sativa oil modulates the therapeutic efficacy of mesenchymal stem cells against liver injury in irradiated rats. J Photochem Photobiol B, 178, 447-456. 12. Benkovica V., Knezevica AH., Dikica D., Lisicic D., Orsolic N., Basic I., kosalec I., Kopjar N., 2008. Radioprotective effects of propolis and quercetin in gamma-irradiated mice evaluated by the alkaline comet assay. Phytomedicine, 15, 851-858. 13. Placer ZA., Cushman LL., Johnson BC., 1966. Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical systems. Anal Biochem, 16, 359-364. 14. Sedlak J., Lindsay RH., 1968. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem, 25, 192-205. 15. Lawrence RA., Burk RF., 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun, 71, 952-958. 16. Sun Y., Oberley WL., Li Y., 1988. A simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500. 17. Lowry OH., Rosebrough NJ., Farr AL., Randall RJ., 1951. Protein measurement with folin phenol reagent. J Biol Chem, 193, 265-275. 18. Cuzzocrea S., Reiter RJ., 2001. Pharmacological action of melatonin in shock, inflammation and ischemia/reperfusion injury. Europ J Pharmacol, 426, 1-10. 19. Li J., Feng L., Xing Y., Wang Y., Du L., Xu C., Cao J., Wang Q., Fan S., Liu Q., Fan F., 2014. Radioprotective and antioxidant effect of resveratrol in hippocampus by activating Sirt1. Int J Mol Sci, 15, 5928-5939. 20. de Freitas RB., Boligon AA., Rovani BT., Piana M., de Brum TF., da Silva Jesus R., Rother FC., Alves NM., Teixeira da Rocha JB., Athayde ML., Barrio JP., de Andrade ER., de Freitas Bauerman L., 2013. Effect of black grape juice against heart damage from acute gamma TBI in rats. Molecules, 18, 12154-12167. 21. Xie Y., Zhao QY., Li HY., Zhou X., Liu Y., Zhang H., 2014. Curcumin ameliorates cognitive deficits heavy ion irradiation-induced learning and memory deficits through enhancing of Nrf2 antioxidant signaling pathways. Pharmacol Biochem Behav, 126, 181-186. 22. Karami M., Asri-Rezaei S., Dormanesh B., Nazarizadeh A., 2018. Comparative study of radioprotective effects of selenium nanoparticles and sodium selenite in irradiation-induced nephropathy of mice model. Int J Radiat Biol, 94, 17-27. 23. Sener G., Kabasakal L., Atasoy BM., Erzik C., Velioğlu-Oğünç A., Cetinel S., Gedik N., Yeğen BC., 2006. Ginkgo biloba extract protects against ionizing radiation-induced oxidative organ damage in rats. Pharmacol Res, 53, 241-252. 24. Ekici K., Temelli O., Parlakpinar H., Samdanci E., Polat A., Beytur A., Tanbek K., Ekici C., Dursun IH., 2016. Beneficial effects of aminoguanidine on radiotherapy-induced kidney and testis injury. Andrologia, 48, 683-692. 25. Cheng C., Yi J., Wang R., Cheng L., Wang Z., Lu W., 2018. Protection of Spleen Tissue of γ-ray Irradiated Mice against Immunosuppressive and Oxidative Effects of Radiation by Adenosine 5'-Monophosphate. Int J Mol Sci, 19, E1273. 26. Said RSI., Nada AS., El-Demerdash E., 2012. Sodium selenite improves folliculogenesis in radiation-induced ovarian failure: a mechanistic approach. PLoS One, 7, e50928. 27. Simsek Y., Gurocak S., Turkoz Y., Akpolat N., Celik O., Ozer A., Yılmaz E., Turhan U., Ozyalın F., 2012. Ameliorative effects of resveratrol on acute ovarian toxicity induced by total body irradiation in young adult rats. J Pediatr Adolesc Gynecol, 25, 262-266. 28. Hermes-Lima M., Storey JM., Storey KB., 1998. Antioxidant defenses and metabolic depression. The hypothesis of preparation for oxidative stress in land snails. Comp Biochem Physiol B-Biochem Mol Biol, 120, 437-448. 29. Black HS., 2004. Reassessment of a free radical theory of cancer with emphasis on ultraviolet carcinogenesis. Integr Cancer Ther, 3, 279-293. 30. Kucukoglu K., Nadaroglu H., 2014. Assessment of the antioxidant and antiradicalic capacities in vitro of different phenolic derivatives. Free Rad Antiox, 4, 24-31. 31. Simsek GI., Gurocak S., Karadag N., Karabulut AB., Demirtas E., Karataş E., Pepele E., 2012. Protective effects of resveratrol on salivary gland damage induced by total body irradiation in rats. Laryngoscope, 122, 2743-2748. 32. Xu L., Yang X., Cai J., Ma J., Cheng H., Zhao K., Yang L., Cao Y., Qin Q., Zhang C., Zhang Q., Sun X., 2013. Resveratrol attenuates radiation-induced salivary gland dysfunction in mice. Laryngoscope,123, E23-29. 33. Saada HN., Said UZ., Meky NH., Abd EI., Azime AS., 2009. Grape seed extract Vitis vinifera protects against radiation-induced oxidative damage and metabolic disorders in rats. Phytother Res, 23, 434-438. 34. Cho BO., Che DN., Shin JY., Kang HJ., Jang SI., 2018. Ameliorative effects of fruit stem extract from Muscat Bailey A against chronic UV-induced skin damage in BALB/c mice. Biomed Pharmacother, 97,1680-1688.
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Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Araştırma Makaleleri |
| Authors | |
| Publication Date | December 31, 2020 |
| Published in Issue | Year 2020 Volume: 15 Issue: 3 |