Sıçanlarda Nikel Genotoksikasyonuna Karşı Bromelainin Etkisi

Year 2022, Volume: 17 Issue: 1, 26 - 30, 29.04.2022

Füsun Temamoğulları , Pinar Aksu Kılıçle , Şükrü Gürler , Zozan Garip

Abstract

Bu çalışmanın amacı, nikel sülfat genotoksikasyonuna bromelainin karşı koruyucu rolünü araştırmaktır. Çalışmada, ortalama 200 ± 20 gr ağırlığında yirmi dört sağlıklı yetişkin erkek Sprague dawley sıçan kullanıldı. Rastgele olacak şekilde dört grup (n = 6) oluşturuldu. Sıçanlar bir kontrol ve üç deney grubu: Grup 1 (Kontrol), Grup 2 (Nikel sülfat 20 mg/kg, ip), Grup 3 (Bromelain 20 mg/kg, oral gavaj), Grup 4 (Nikel sülfat, 20 mg/kg, ip + bromelain, 20 mg/kg, oral gavaj). On günlük deney periyodundan sonra hayvanlar on birinci gün ötenazi edildi. 11. günde ötenazi edilen hayvanlardan femur kemikleri alındı ve mikronükleus protokolü uygulandı. Protokolün sonunda MNPCE (mikronükleuslu polikromatik eritrositler) ve PCE (polikromatik eritrositler) sayıldı. Sayılan değerlerin istatistiksel sonuçları belirlendi. Tüm gruplar sadece MNCPE parametresine göre karşılaştırıldı. MNCPE seviyesi, bromelainli grupta, sadece nikel sülfatlı grubuna kıyasla istatistiksel olarak anlamlıydı (P < ,05). Elde edilen veriler, bromelainin 20 mg/kg dozunda uygulanması, 20 mg/kg dozunda uygulanan nikelsülfatin oluşturduğu genotoksik etkilerini potansiyel olarak önleyici olmadığını göstermiştir.

Keywords

Bromelain, Genotoksisite, Nikel sülfat, Rat

Supporting Institution

HARRAN ÜNİVERSİTESİ - BİLİMSEL ARAŞTIRMA PROJELERİ BİRİMİ

Project Number

18029

Effect of Bromelain Against Nickel Genotoxication in Rats

Abstract

In this study, we aimed to investigate the protective role of bromelain against nickel sulfate genotoxication. Twenty-four healthy adult male Sprague Dawley rats with an average weight of 200 ± 20 g were used in the study. These were divided randomly into four groups (n = 6), including one control and three experimental groups: Group 1 (control), Group 2 (nickel sulfate 20 mg/kg, intraperitoneal [IP]), Group 3 (Bromelain 20 mg/kg, oral gavage ), Group 4 (nickel sulfate 20 mg/kg IP + bromelain 20 mg/kg oral gavage ). After the ten-day experimental period, the animals were euthanized on the 11th day. Femoral bones were taken from animals dissected on day 11, and micronucleus protocol was applied. At the end of the protocol, micronucleated polychromatic erythrocytes (MNPCE) and polychromatic erythrocytes (PCE) were counted. The statistical results of the counted values were determined. The groups were compared only according to MNCPE, the level of which was statistically significant in the group with bromelain than in the group with nickel sulfate alone (P < .05). The data obtained suggested that bromelain at the administered dose (20 mg/kg) is not potentially preventive to the genotoxic effects of nickel sulfate at the administered dose (20 mg/kg).

Keywords

Bromelain, Genotoxicity, Nickel sulphate, Rat

Project Number

18029

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