Lead Biosorption by a Moderately Halophile Penicillium sp. Isolated from Çamalti Saltern in Turkey

Year 2016, Volume: 5 Issue: 1, 13 - 22, 13.05.2016

Mine Aydın Kurç , Kiymet Güven , Elif Korcan , Alaettin Güven , Semra Malkoc

https://doi.org/10.18036/btdc.01000

Abstract

Owing the importance of biosorption of heavy metals by different organisms, a moderately halophilic fungus isolated from Çamalti saltern was first time investigated for its potential for biosorption. Different heavy metals namely, lead [(Pb(NO₃)₂], nickel (NiCl₂), chromium (K₂CrO₄), zinc (ZnCl₂), cadmium (CdCl₂.H₂O), copper (CuSO₄) and cobalt (CoCl₂.6H₂O) were screened for resistance and the most tolerated heavy metal by Penicillium sp. was chosen in biosorption assay.

The heavy metal tolerance of Penicillium sp. was observed in order of lead>cadmium >chromium>copper>nickel>zinc>cobalt. Different concentrations (145 mg/l, 644 mg/l and 1388 mg/l) of lead biosorption was investigated and increasing the metal ion concentration resulted in decreased uptake for lead. Freundlich isotherm was more effective than Langmuir isotherm for lead biosorption by Penicillium sp. The binding sites for lead attributed to the amine groups on the biomass surface were verified by Fourier Transform Infra Red (FTIR) analysis.

A halotolerant Penicillium sp. having high resistance to lead, could be suggested for use as an agent for abatement of lead pollution in hypersaline conditions or in waters of fluctuating salinity, as well as in non-saline environments after further studies of optimization.

LEAD BIOSORPTION BY A MODERATELY HALOPHILE PENICILLIUM SP. ISOLATED FROM ÇAMALTI SALTERN IN TURKEY

Abstract

Ağır metallerin farklı organizmalarla biyosorbsiyonunun önemi sebebiyle Çamaltı tuzlasından izole edilen tuza toleranslı (halotolerant) fungusun biyosorbsiyon potansiyeli bu çalışma ile ilk kez araştırılmıştır. Penicillium sp. izolatının kurşun [(Pb(NO3)2], nikel (NiCl2), krom (K2CrO4), çinko (ZnCl2), kadmiyum (CdCl2.H2O), bakır (CuSO4) ve kobalt (CoCl2.6H2O) ağır metallerine dirençlilikleri araştırılmış ve Penicillium sp. nin en yüksek tolerans gösterdiği ağır metal biyosorpsiyon çalışmaları için seçilmiştir

Keywords

Biyosopsiyon, Kurşun, Penicillum sp., Ilımlı halofil

References

• Melgar MJ, Alonso J, Garcia MA. Removal of toxic metals from aqueous solutions by fungal biomass of Agaricus macrosporus. Sci Total Environ 2007; 385: 12–19.
• Amini M, Younesi H, Bahramifar N, Lorestani AAZ, Ghorbani F, Daneshi A, Sharifzadeh M. Application of response surface methodology for optimization of lead biosorption in an aqueous solution by Aspergillus niger. J Hazard Mater 2008; 154: 694–702.
• Tunali S, Çabuk A, Akar T. Removal of lead and copper ions from aqueous solutions by bacterial strain isolated from soil. Chem Eng J 2006; 115: 203–211.
• Sheng PX, Ting Y-P, Chen JP, Hong L. Sorption of lead, copper, cadmium, zinc, and nickel by marine algal biomass: characterization of biosorptive capacity and investigation of mechanisms. J Colloid Interf Sci 2004; 275: 131–141.
• Hussein H, Farag S, Kandil K, Moavad H. Tolerance and uptake of heavy metals by Pseudomonads. Process Biochem 2005; 40: 955–961.
• Liu H-S, Chen B-Y, Lan Y-W, Cheng Y-C. Biosorption of Zn (II) and Cu (II) by the indigenous Thiobacillus thiooxidans. Chem Eng J 2004; 97: 195–201.
• Dönmez G, Aksu Z. Removal of chromium (VI) from saline wastewaters by Dunaliella species. Process Biochem 2002; 38: 751–762.
• Çabuk A, Akar T, Tunali S, Gedikli S. Biosorption of Pb (II) by industrial strain of Saccharomyces cerevisiae immobilized on the biomatrix of cone biomass of Pinus nigra: equilibrium and mechanism analysis. Chem Eng J 2007; 131: 293–300.
• Nuhoglu Y, Oguz E. Removal of copper (II) from aqueous solutions by biosorption on the cone biomass of Thuja orientalis. Process Biochem. 2003; 38: 1627–1631.
• Ansari, MI, Malik A. Biosorption of nickel and cadmium by metal resistant bacterial isolates from agricultural soil irrigated with industrial wastewater. Bioresource Technol 2007; 98: 3149–3153. [11] Malik A. Metal bioremediation through growing cells. Environ Int 2004; 30: 261– 278.
• Oren A. Diversity of halophilic microorganisms: environments, phylogeny, physiology and applications. J Ind Microbiol Biot 2002; 28: 56–63.
• Abdel-Hafez SII. Halophilic fungi of desert soils in Saudi Arabia. Mycopathologia 1981; 75: 75–80. [14] Buchalo AS, Nevo E, Wasser SP, Oren A, Molitoris HP. Fungal life in the extremely hypersaline water of the Dead Sea: First Records. Proc Royal Soc London Biol Sci 1998; 265: 1461–1465.
• Kis-Papo T, Grishkan I, Ren A, Wasser SP, Nevo E. Spatiotemporal diversity of filamentous fungi in the hypersaline Dead Sea. Mycol Res 2001; 105: 749–756.
• Grishkan I, Nevo E, Wasser, PS. Soil micromycete diversity in the hypersaline Dead Sea coastal area, Israel, Mycol Prog 2003; 2: 19–28.
• Cantrell SA, Casillaz-Martinez L, Molina M. Characterization of fungi from hypersaline environments of solar salterns using morphological and molecular techniques. Mycol Res 2006; 110: 962–970.
• Marbaniang T, Nazareth S. Isolation of halotolerant Penicilium species from mangroves and salterns and their resistance to heavy metals. Curr Sci India 2007; 92: 895–897.
• Zafar S, Aqil F, Ahmad L. Metal tolerance and biosorption potential of filamentous fungi isolated from metal contaminated agricultural soil. Bioresource Technol 2007; 98: 2557-2561.
• Moore BA, Duncan JR, Burgess JE. Fungal bioaccumulation of copper, nickel, gold and platinum. Miner Eng 2008; 21: 55–60.
• Dakiky M, Khamis M, Manassra A, Mer’eb M. Selective adsorption of chromium (VI) in industrial wastewater using low-cost abundantly available adsorbents. Adv Environ Res 2002; 6: 533–540.
• Plaza G, Lukasik W, Ulfig K. Sorption of cadmium by filamentous soil fungi. Acta Microbiol Pol 1996; 45: 193–201.
• Kapoor A, Viraraghavan T. Heavy metal biosorption sites in Aspergillus niger. Bioresource Technol 1997; 61: 221–227.
• Kapoor A, Viraraghavan T. Biosorption of heavy metals on Aspergillus niger: effect of pretreatment. Bioresource Technol 1998; 63: 109–113.
• Park D, Yun YS, Park JM. Use of dead fungal biomass for the detoxification of hexavalent chromium: screening and kinetics. Process Biochem 2005; 40: 2559–2565.
• Barros LM Jr, Macedo GR,.Duarte MML, Silva EP, Lobato AKCL. Biosorption of cadmium using the fungus Aspergillus niger Braz. J Chem Eng 2003; 20: 1-17.
• Kumar R, Bishnoi, NR, Bishnoi, GK. Biosorption of chromium (VI) from aqueous solution and electroplating wastewater using fungal biomass. Chem Eng J 2008; 135: 202–208.
• Niu H, Xu XS, Wang JH. Removal of lead from aqueous solutions by Penicillin biomass. Biotechnol Bioeng 1993; 42: 785–787.
• Holan ZR, Volesky B. Accumulation of cadmium, lead, and nickel by fungal and wood biosorbents. Appl Biochem Biotech. 1995; 53: 133–146.
• Lo W, Chua H, Lam KH, Bi SP. A comparative investigation on the biosorption of lead by filamentous fungal biomass. Chemosphere 1999; 39: 2723–2736.
• Tan T, Cheng P. Biosorption of metal ions with Penicillium chrysogenum. Appl Biochem Biotech 2003; 104: 119–128.
• Fan T, Liu Y, Feng B, Zeng G, Yang C, Zhou M, Zhou H, Tan Z, Wang X. Biosorption of cadmium (II), zinc (II) and lead (II) by Penicillium simplicissimum: Isotherms, kinetics and thermodynamics. J Hazard Mater 2008; 160: 655–661.
• Rostami KH, Joodaki MR. Some studies of cadmium adsorpion using Aspergillus niger, Penicillium austurianum, employing an airlift fermenter. Chem Eng J 2002; 89: 239–252.
• Muraleedharan TR. Venkobachar LI. Further insight into the mechanism of biosorption of heavy metals by Ganoderma lucidum. Environ Technol 1994; 15: 1015–1027.