AN INVESTIGATION OF MUTAGENIC ACTIVITIES OF SOME 9- SUBSTITUEDPHENANTHRENE DERIVATIVES WITH AMES / SALMONELLA / MICROSOME TEST

Year 2011, Volume: 1 Issue: 1, 83 - 94, 09.02.2011

Mehtap Kutlu , Esin Öztaş Gözde Aydoğan Kılıç , İlhan Işıkdağ Yusuf Özkay

Abstract

In this study, three different 9-Substituted phenantrene derivatives that were synthesized to be
used as basic matter for drugs were tested for their mutagenic potency in strains TA 98 and TA 100 of
Salmonella typhimurium by using Ames test. Therefore, both strains were tested in the absence or
presence of S9 metabolic activation, for five different doses of each test substances in two paralel independent
experiments. In the absence of metabolic activation, while only one of the compounds were
causing frame-shift mutations, two of them were determined to induce base pair substitution mutations.
In the presence of metabolic activation, while two of the compounds were mutagenic for TA 98,
base pair substitution mutations were not detected for any of the substances. Each of the compounds
tested were found to be mutagenic for at least one strain in the presence or absence of S9.

Keywords

Ames test, Phenanthrene derivatives, Mutagenecity

Yaşam Bilimleri ve Biyoteknoloji

Abstract

Bu çalışmada, ilaç ön maddesi olarak sentezlenmiş olan üç farklı 9-Sübstitüye fenantren türevi, Ames test yöntemi kullanılarak, Salmonella typhimurium TA 98 ve TA 100 suşları yardımı ile mutajenik potansiyelleri açısından test edilmiştir. Bunun için, her iki suş, mikrozomal enzimler içeren metabolik aktivasyon (S9) varlığında ve yokluğunda, her bir test maddesinin beş ayrı dozu ile iki bağımsız deneyle araştırılmıştır. Metabolik aktivasyon yokluğunda, sentezlenen bileşiklerden yalnızca bir tanesi çerçeve kayması mutasyonlarına neden olurken, ikisinin baz çifti değişimi mutasyonlarını indüklediği belirlenmiştir. Metabolik aktivasyonlu ortamda ise iki madde TA 98 suşu için mutajen özellik gösterirken, baz çifti değişimi mutasyonu hiçbir madde için tespit edilmemiştir. Test edilen bileşiklerin her birinin, S9 varlığında veya yokluğunda, en az bir şuş için mutajenik özellikte olduğu tespit edilmiştir

Keywords

Ames test, Fenantren türevleri, Mutajenite

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